Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   LXG94_RS25720 Genome accession   NZ_CP090179
Coordinates   3290067..3290531 (-) Length   154 a.a.
NCBI ID   WP_005446258.1    Uniprot ID   -
Organism   Vibrio harveyi strain XH2145     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3285067..3295531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LXG94_RS25700 (LXG94_25700) coaE 3285564..3286178 (-) 615 WP_038897988.1 dephospho-CoA kinase -
  LXG94_RS25705 (LXG94_25705) pilD 3286179..3287048 (-) 870 WP_005446255.1 A24 family peptidase Machinery gene
  LXG94_RS25710 (LXG94_25710) pilC 3287121..3288344 (-) 1224 WP_005446256.1 type II secretion system F family protein Machinery gene
  LXG94_RS25715 (LXG94_25715) pilB 3288378..3290063 (-) 1686 WP_005446257.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LXG94_RS25720 (LXG94_25720) pilA 3290067..3290531 (-) 465 WP_005446258.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  LXG94_RS25725 (LXG94_25725) nadC 3290796..3291683 (-) 888 WP_029790346.1 carboxylating nicotinate-nucleotide diphosphorylase -
  LXG94_RS25730 (LXG94_25730) ampD 3291776..3292327 (+) 552 WP_038897989.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  LXG94_RS25735 (LXG94_25735) pdhR 3292732..3293499 (+) 768 WP_009699648.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16024.27 Da        Isoelectric Point: 5.8347

>NTDB_id=640997 LXG94_RS25720 WP_005446258.1 3290067..3290531(-) (pilA) [Vibrio harveyi strain XH2145]
MKTNKQKKQQGFTLIELMIVVAIIGILAAFAVPAYQNYTKKATLAEFPKAAAAMKLAVELCAHENASDAATFKTNCLSGS
NGVPSKIADLNDISIEAVAGSSGVNVIAKAIAVKGPIAANEQYTMASNYTASGIEWTGSCQDSGGNAQTDYCPE

Nucleotide


Download         Length: 465 bp        

>NTDB_id=640997 LXG94_RS25720 WP_005446258.1 3290067..3290531(-) (pilA) [Vibrio harveyi strain XH2145]
ATGAAAACGAATAAACAGAAGAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATTGTGGTTGCAATTATTGGTATTTT
GGCTGCATTTGCAGTACCTGCATATCAAAACTATACAAAAAAAGCGACATTAGCGGAGTTCCCAAAAGCAGCAGCAGCTA
TGAAGTTAGCAGTTGAATTGTGCGCGCACGAAAATGCTTCAGATGCAGCGACCTTTAAAACAAACTGCCTTTCTGGTAGC
AATGGAGTGCCTTCAAAGATTGCTGATTTAAACGATATATCTATTGAAGCTGTCGCTGGTTCCTCTGGCGTAAATGTCAT
TGCAAAAGCAATTGCAGTGAAAGGACCTATCGCAGCTAATGAACAATACACAATGGCATCAAACTACACTGCAAGTGGTA
TTGAATGGACTGGCTCTTGTCAGGATTCTGGGGGGAACGCTCAGACTGATTACTGTCCTGAATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

56.774

100

0.571
  pilA Haemophilus influenzae 86-028NP

41.958

92.857

0.39
  pilA2 Legionella pneumophila strain ERS1305867

39.865

96.104

0.383
  pilA2 Legionella pneumophila str. Paris

39.865

96.104

0.383