Detailed information NTG0640910

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: LXH20_RS12345
• Genome accession: NZ_CP090150
• Coordinates: 2551263..2551877 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain BR-01
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2546263..2556877

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LXH20_RS12315 (LXH20_12315)	rpsT	2546566..2546832 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
LXH20_RS12320 (LXH20_12320)	holA	2546849..2547890 (-)	1042	Protein_2383	DNA polymerase III subunit delta	-
LXH20_RS12325 (LXH20_12325)	-	2547930..2548081 (-)	152	Protein_2384	hypothetical protein	-
LXH20_RS12330 (LXH20_12330)	-	2548122..2548256 (+)	135	WP_003152870.1	YqzM family protein	-
LXH20_RS12335 (LXH20_12335)	comEC	2548275..2550626 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
LXH20_RS12340 (LXH20_12340)	-	2550627..2551196 (-)	570	WP_003152868.1	ComE operon protein 2	-
LXH20_RS12345 (LXH20_12345)	comEA	2551263..2551877 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
LXH20_RS12350 (LXH20_12350)	comER	2551936..2552757 (+)	822	WP_014418420.1	late competence protein ComER	-
LXH20_RS12355 (LXH20_12355)	-	2552826..2553563 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
LXH20_RS12360 (LXH20_12360)	rsfS	2553560..2553916 (-)	357	WP_007408260.1	ribosome silencing factor	-
LXH20_RS12365 (LXH20_12365)	yqeK	2553934..2554494 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
LXH20_RS12370 (LXH20_12370)	-	2554484..2555053 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
LXH20_RS12375 (LXH20_12375)	yhbY	2555064..2555354 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
LXH20_RS12380 (LXH20_12380)	aroE	2555348..2556190 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=640910 LXH20_RS12345 WP_014418419.1 2551263..2551877(-) (comEA) [Bacillus velezensis strain BR-01]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=640910 LXH20_RS12345 WP_014418419.1 2551263..2551877(-) (comEA) [Bacillus velezensis strain BR-01]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363