Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LVB77_RS18245 Genome accession   NZ_CP089924
Coordinates   4050830..4052557 (+) Length   575 a.a.
NCBI ID   WP_232907487.1    Uniprot ID   -
Organism   Lysobacter sp. 5GHs7-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4045830..4057557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVB77_RS21315 - 4045961..4046389 (+) 429 WP_305068908.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  LVB77_RS18235 (LVB77_18235) - 4046579..4048540 (+) 1962 WP_232907483.1 hypothetical protein -
  LVB77_RS18240 (LVB77_18240) - 4048747..4050681 (+) 1935 WP_232907485.1 hypothetical protein -
  LVB77_RS18245 (LVB77_18245) pilB 4050830..4052557 (+) 1728 WP_232907487.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVB77_RS18250 (LVB77_18250) pilC 4052561..4053814 (+) 1254 WP_232907488.1 type II secretion system F family protein Machinery gene
  LVB77_RS18255 (LVB77_18255) pilD 4053838..4054701 (+) 864 WP_232907490.1 A24 family peptidase Machinery gene
  LVB77_RS18260 (LVB77_18260) coaE 4054707..4055318 (+) 612 WP_232907491.1 dephospho-CoA kinase -
  LVB77_RS18265 (LVB77_18265) - 4055466..4056425 (-) 960 WP_232907492.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62275.63 Da        Isoelectric Point: 6.1530

>NTDB_id=639116 LVB77_RS18245 WP_232907487.1 4050830..4052557(+) (pilB) [Lysobacter sp. 5GHs7-4]
MSSVATANLVGITGIARRLVLDGALEEAAAREAMAAASGERKPIATYLSEKRLVSAGQLAAANSIEFGVPLFDASVLDQA
QSAVKLVSEELVRKHAVLPLFKRGNKLFVGLADPTNTRALDEIKFQTNLVIEAILVDEDTIRRSLDKWLESADALGDGVN
EEGLETLDVGGGDDDLTAAGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPVK
LSPRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKIVLRILDGSAARLGVDKLGYEEDQKELF
VGAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDDMRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFSHDEIVGGLKVYEPAGCPDCTEGYKGRTGIYQVMPMTEQIQAIVLEGGNAMQIAAAAQKSGVRDLRQSALMKVR
NGVTSLSEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=639116 LVB77_RS18245 WP_232907487.1 4050830..4052557(+) (pilB) [Lysobacter sp. 5GHs7-4]
ATGTCTAGCGTCGCTACCGCCAACTTGGTGGGGATCACCGGCATTGCCCGGCGCTTGGTGCTGGACGGCGCCCTGGAAGA
AGCCGCGGCCCGCGAGGCCATGGCCGCCGCCAGCGGCGAGCGCAAGCCGATCGCGACCTATCTGTCCGAAAAGCGACTGG
TCTCGGCCGGTCAGCTCGCGGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACGCCTCGGTGCTCGACCAGGCC
CAGTCGGCGGTCAAGCTGGTCAGCGAAGAGCTGGTGCGCAAACACGCCGTGCTGCCGCTGTTCAAGCGCGGCAACAAGCT
GTTCGTGGGCCTGGCCGATCCGACCAACACCCGCGCGCTGGACGAGATCAAGTTTCAGACCAACCTGGTGATCGAGGCCA
TCCTCGTCGACGAGGACACGATCCGCCGCAGCCTGGACAAGTGGCTGGAGTCGGCCGACGCGCTGGGCGACGGCGTCAAC
GAGGAAGGGCTGGAAACGCTCGATGTCGGTGGTGGCGACGACGACCTGACCGCCGCCGGCGACAGCGGCGTCGACGCCAA
GGGCGACGACACCCCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCCGCGGCGCCTCGGACATCCACT
TCGAGCCCTACGAAACCGACTATCGCGTGCGCCTGCGCATCGACGGCCTACTCAAGCAGGTCGCCAAGGTGCCGGTCAAG
CTGAGCCCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTGGACATCGCCGAGAAGCGCGTCCCGCAGGACGGCCG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCCGCGCGCCTGGGCGTGGACAAGCTGGGTTACGAGGAAGACCAGAAGGAGCTGTTC
GTCGGCGCCGTGGTCAAGCCCTACGGCATGGTGCTGGTGACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGAACATCCTCAACGACGACATGCGCAACATCTCCACCGTCGAGGACCCGGTCGAAATCCGCGTGCCCGGCATCA
ACCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGACAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTGGAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTC
CACCCTGCACACCAACGACGCCCCGCAGACCATCGCGCGCCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGT
CGGTGACCCTGGTCATCGCCCAGCGACTGGCCCGCCGCCTGCACGACTGCAAGCGCGAAGTCCATCTGCCCGAGCACGCG
CTGCTGGCCGAGGGCTTCAGCCACGACGAGATCGTCGGTGGGCTCAAGGTTTACGAGCCCGCCGGCTGCCCGGACTGCAC
CGAGGGCTACAAGGGCCGTACCGGCATCTACCAGGTCATGCCCATGACCGAACAGATCCAGGCCATCGTGCTGGAGGGCG
GCAACGCGATGCAGATCGCGGCGGCCGCTCAGAAGTCCGGCGTCCGCGACCTGCGCCAATCGGCGTTGATGAAGGTCAGG
AACGGCGTGACCAGCCTCTCCGAAATCAACCGCGTTACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(199-466)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.75

100

0.551
  pilB Acinetobacter baylyi ADP1

54.497

98.609

0.537
  pilB Legionella pneumophila strain ERS1305867

50.615

98.957

0.501
  pilB Vibrio cholerae strain A1552

49.913

99.652

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

49.54

94.435

0.468
  pilF Neisseria gonorrhoeae MS11

47.584

93.565

0.445
  pilB Vibrio campbellii strain DS40M4

49.023

89.043

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.532

100

0.377