Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DYST_RS09715 Genome accession   NZ_CP089414
Coordinates   2264734..2266455 (+) Length   573 a.a.
NCBI ID   WP_239951581.1    Uniprot ID   -
Organism   Dyella terrae strain Ely Copper Mine     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2259734..2271455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DYST_RS09695 (DYST_01987) - 2260035..2261081 (-) 1047 WP_239951577.1 glycosyltransferase -
  DYST_RS09700 (DYST_01988) - 2261227..2262345 (-) 1119 WP_275666953.1 UDP-N-acetylglucosamine 2-epimerase -
  DYST_RS09705 (DYST_01989) - 2262348..2263202 (-) 855 WP_239951579.1 glycosyltransferase family 2 protein -
  DYST_RS09710 (DYST_01990) - 2263332..2264504 (-) 1173 WP_239951580.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  DYST_RS09715 (DYST_01991) pilB 2264734..2266455 (+) 1722 WP_239951581.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DYST_RS09720 (DYST_01992) pilC 2266614..2267876 (+) 1263 WP_239951582.1 type II secretion system F family protein Machinery gene
  DYST_RS09725 (DYST_01993) - 2267948..2268805 (+) 858 WP_239951583.1 A24 family peptidase -
  DYST_RS09730 (DYST_01994) - 2268817..2270538 (+) 1722 WP_239951584.1 sulfatase-like hydrolase/transferase -
  DYST_RS09735 (DYST_01995) coaE 2270535..2271167 (+) 633 WP_239951586.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62633.33 Da        Isoelectric Point: 6.5145

>NTDB_id=636858 DYST_RS09715 WP_239951581.1 2264734..2266455(+) (pilB) [Dyella terrae strain Ely Copper Mine]
MSTLMQPSLAGLTGLARRLVSEDVLPEADVRRAVAESTQSKTSLAAWLLDNSLVESARLTRIASDEFGMPMMDVSALAIP
SMPVNLISEALIKKHHALPLFKRGKRLFVGIADPMQSHALDEIKFHSNCMVEPILVERSQLLKVIDTALTALSNAVPDMG
NSEFDELSLEGGDEEAESTGIDANANDDAPVVKFVNKILVDAIKRGASDIHFEPFETQYRVRLRMDGMLRAVASPPMKLA
ARISSRIKVMSGLDIAERRVPQDGRIKLNLSKSRSVDFRVSTLPTLFGEKIVLRILDGSAAKLGIDVLGYEDAQKQLYVD
AIHKPYGMVLVTGPTGSGKTVSLYTALNILNTNERNISTVEDPVEIRVEGINQVQQNVKRGMTFAAALRSFLRQDPDVIM
VGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPYNITSSVTLVIAQRLARRLHDCKRPINLPPQVLL
DAGFTQEEIDAGLTVYEAVGCDNCNEGYKGRLGIYQVMPMLEEIQKIILHGGNTLQIGEAARRAGVNDLRASALLKVKKG
VTSLAEIDRVTKD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=636858 DYST_RS09715 WP_239951581.1 2264734..2266455(+) (pilB) [Dyella terrae strain Ely Copper Mine]
ATGTCAACGCTAATGCAACCCTCGCTCGCGGGCCTCACCGGCCTTGCGCGCAGGCTGGTATCGGAAGACGTACTGCCCGA
GGCGGACGTGCGCCGGGCGGTTGCCGAGTCAACCCAGAGCAAGACCTCGCTCGCGGCTTGGTTGCTGGACAACAGTCTGG
TGGAGAGCGCGCGACTCACCCGTATCGCATCCGACGAGTTCGGCATGCCGATGATGGACGTCAGCGCGCTGGCCATCCCC
AGCATGCCCGTGAACCTGATCAGCGAGGCGCTGATCAAGAAGCACCACGCCCTCCCCCTGTTCAAGCGCGGCAAGCGCCT
GTTCGTCGGCATCGCCGATCCCATGCAGTCGCATGCACTGGACGAGATCAAGTTCCACTCCAATTGCATGGTGGAACCGA
TCCTCGTCGAACGTAGCCAACTGCTGAAGGTTATCGACACTGCTCTCACAGCCCTGAGCAACGCTGTACCCGACATGGGC
AACAGCGAATTCGACGAGCTTTCGCTAGAAGGCGGCGACGAAGAAGCCGAATCCACCGGCATCGATGCCAACGCGAATGA
CGATGCACCCGTCGTCAAGTTCGTCAATAAAATCCTGGTCGATGCCATCAAGCGTGGCGCGTCCGATATCCACTTCGAAC
CGTTCGAAACGCAATACCGCGTGCGCCTGCGCATGGATGGCATGCTGCGCGCCGTCGCCAGCCCACCGATGAAGCTCGCC
GCACGCATCTCGTCGCGCATCAAGGTGATGTCCGGCCTCGATATCGCGGAACGTCGCGTACCGCAGGACGGCCGCATCAA
GCTCAACCTGTCCAAGAGCCGTTCGGTCGACTTCCGCGTGAGTACACTGCCCACGCTGTTCGGCGAGAAGATCGTGTTGC
GTATTCTCGACGGCTCGGCAGCCAAGCTCGGCATCGACGTCCTGGGCTACGAGGACGCGCAGAAGCAGTTGTACGTTGAC
GCCATCCACAAGCCCTACGGCATGGTGTTAGTCACCGGCCCTACGGGCTCGGGTAAAACGGTATCGCTGTATACAGCACT
CAACATCCTCAACACCAACGAGCGGAACATCTCGACGGTGGAGGACCCGGTGGAAATCCGCGTCGAGGGCATTAACCAGG
TCCAGCAAAACGTCAAGCGCGGTATGACCTTCGCCGCCGCCCTGCGCTCCTTCCTGCGTCAGGATCCGGACGTGATCATG
GTGGGCGAAATCCGTGACCTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACCGGCCATATGGTGCTGTCGACCCT
GCACACCAACGATGCACCGCAGACGATCTCCCGCCTGATGAACATGGGTATCGCGCCCTACAACATCACCTCGTCAGTGA
CCCTAGTCATCGCCCAGCGACTGGCGCGCCGCCTGCACGACTGCAAGCGGCCGATCAACCTGCCGCCGCAGGTGCTGCTG
GACGCCGGCTTTACCCAGGAAGAGATCGACGCCGGCCTAACCGTCTACGAGGCAGTAGGTTGCGACAACTGCAATGAGGG
CTACAAAGGCCGCCTGGGTATCTACCAAGTGATGCCCATGCTGGAGGAAATCCAGAAGATCATCCTGCACGGCGGCAACA
CACTGCAGATCGGGGAGGCTGCCCGCAGGGCCGGCGTGAACGATCTGCGTGCCTCCGCCTTGCTCAAGGTCAAGAAAGGC
GTGACCAGCCTGGCCGAGATCGACCGCGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(19-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.69

98.604

0.539
  pilB Acinetobacter baylyi ADP1

53.043

100

0.532
  pilB Legionella pneumophila strain ERS1305867

51.67

99.302

0.513
  pilB Vibrio cholerae strain A1552

48.41

98.778

0.478
  pilF Neisseria gonorrhoeae MS11

48.404

98.429

0.476
  pilB Vibrio campbellii strain DS40M4

47.173

98.778

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

48.513

93.892

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.321

95.637

0.366