NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	N8153_RS20065	Genome accession	NZ_CP106756
Coordinates	4130329..4130988 (+)	Length	219 a.a.
NCBI ID	WP_024216571.1	Uniprot ID	-
Organism	Escherichia coli strain B-JRPT-4119
Function	repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 4125329..4135988

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N8153_RS20045 (N8153_20045)	plsC	4125475..4126212 (-)	738	WP_000965712.1	1-acylglycerol-3-phosphate O-acyltransferase	-
N8153_RS20050 (N8153_20050)	parC	4126446..4128704 (-)	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
N8153_RS20055 (N8153_20055)	ygiV	4129250..4129732 (-)	483	WP_000183490.1	GyrI-like domain-containing protein	-
N8153_RS20060 (N8153_20060)	ygiW	4129785..4130177 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
N8153_RS20065 (N8153_20065)	ciaR	4130329..4130988 (+)	660	WP_024216571.1	quorum sensing response regulator transcription factor QseB	Regulator
N8153_RS20070 (N8153_20070)	qseC	4130985..4132334 (+)	1350	WP_024216572.1	quorum sensing histidine kinase QseC	-
N8153_RS20075 (N8153_20075)	mdaB	4132444..4133025 (+)	582	WP_001392669.1	NADPH:quinone oxidoreductase MdaB	-
N8153_RS20080 (N8153_20080)	ygiN	4133056..4133370 (+)	315	WP_000633738.1	putative quinol monooxygenase	-
N8153_RS20085 (N8153_20085)	-	4133415..4134302 (-)	888	WP_263082305.1	MurR/RpiR family transcriptional regulator	-
N8153_RS20090 (N8153_20090)	-	4134299..4135246 (-)	948	WP_001305988.1	iron-siderophore ABC transporter substrate-binding protein	-

Sequence

Protein

Download Length: 219 a.a. Molecular weight: 24701.65 Da Isoelectric Point: 6.9850

                                >NTDB_id=636448 N8153_RS20065 WP_024216571.1 4130329..4130988(+) (ciaR) [Escherichia coli strain B-JRPT-4119]

MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPILILTA

RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL

LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

Download Length: 660 bp

                                >NTDB_id=636448 N8153_RS20065 WP_024216571.1 4130329..4130988(+) (ciaR) [Escherichia coli strain B-JRPT-4119]

ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT

CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT

TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGATACTGATCCTGACCGCG

CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA

AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC

TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGTGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA

CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG

TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT

ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus pneumoniae TIGR4	37.885	100	0.393
ciaR	Streptococcus pneumoniae D39	37.885	100	0.393
ciaR	Streptococcus pneumoniae R6	37.885	100	0.393
ciaR	Streptococcus pneumoniae Rx1	37.885	100	0.393
covR	Lactococcus lactis subsp. lactis strain DGCC12653	34.956	100	0.361
micA	Streptococcus pneumoniae Cp1015	34.498	100	0.361
ciaR	Streptococcus mutans UA159	35.426	100	0.361

Detailed information NTG0636448