Detailed information NTG0636075

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LT107_RS14710
• Genome accession: NZ_CP089282
• Coordinates: 3282024..3283193 (-)
• Length: 389 a.a.
• NCBI ID: WP_054071721.1
• Uniprot ID: A0A9P3AB78
• Organism: Pseudomonas amygdali pv. loropetali strain AAC
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3277024..3288193

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LT107_RS14695 (LT107_14695)	-	3278308..3279372 (+)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-
LT107_RS14700 (LT107_14700)	-	3279372..3280394 (+)	1023	WP_005732025.1	ABC transporter permease	-
LT107_RS14705 (LT107_14705)	-	3280396..3281973 (+)	1578	WP_243787016.1	ABC transporter ATP-binding protein	-
LT107_RS14710 (LT107_14710)	pilU	3282024..3283193 (-)	1170	WP_054071721.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LT107_RS14715 (LT107_14715)	-	3283317..3283592 (+)	276	WP_002553334.1	peptidylprolyl isomerase	-
LT107_RS14720 (LT107_14720)	pgm	3283667..3285313 (-)	1647	WP_054071722.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
LT107_RS14725 (LT107_14725)	-	3285429..3287756 (-)	2328	WP_054071723.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42578.79 Da
• Isoelectric Point: 6.0641

>NTDB_id=636075 LT107_RS14710 WP_054071721.1 3282024..3283193(-) (pilU) [Pseudomonas amygdali pv. loropetali strain AAC]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPIGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=636075 LT107_RS14710 WP_054071721.1 3282024..3283193(-) (pilU) [Pseudomonas amygdali pv. loropetali strain AAC]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTAGGCAGTGAAACCCTCAAACCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCGATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTCGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGACCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAACCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGCTGCTGCATGACCTGGGCAACAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAAGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGATCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362