Detailed information NTG0635669

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LTQ03_RS09660
• Genome accession: NZ_CP089201
• Coordinates: 2086502..2088187 (-)
• Length: 561 a.a.
• NCBI ID: WP_243583227.1
• Uniprot ID: -
• Organism: Vibrio splendidus strain 1_C04a
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2080604..2098034	2086502..2088187	within	0

Gene organization within MGE regions

Location: 2080604..2098034

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LTQ03_RS09620 (LTQ03_09620)	rimM	2080604..2081158 (+)	555	WP_004735510.1	ribosome maturation factor RimM	-
LTQ03_RS09625 (LTQ03_09625)	trmD	2081185..2081925 (+)	741	WP_004735512.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
LTQ03_RS09630 (LTQ03_09630)	rplS	2081985..2082338 (+)	354	WP_004735514.1	50S ribosomal protein L19	-
LTQ03_RS09635 (LTQ03_09635)	yacG	2082658..2082855 (-)	198	WP_004735515.1	DNA gyrase inhibitor YacG	-
LTQ03_RS09640 (LTQ03_09640)	zapD	2082937..2083677 (-)	741	WP_017088714.1	cell division protein ZapD	-
LTQ03_RS09645 (LTQ03_09645)	coaE	2083704..2084318 (-)	615	WP_243583225.1	dephospho-CoA kinase	-
LTQ03_RS09650 (LTQ03_09650)	pilD	2084321..2085190 (-)	870	WP_017088712.1	A24 family peptidase	Machinery gene
LTQ03_RS09655 (LTQ03_09655)	pilC	2085258..2086487 (-)	1230	WP_243583226.1	type II secretion system F family protein	Machinery gene
LTQ03_RS09660 (LTQ03_09660)	pilB	2086502..2088187 (-)	1686	WP_243583227.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LTQ03_RS09665 (LTQ03_09665)	pilA	2088190..2088648 (-)	459	WP_243583228.1	pilin	Machinery gene
LTQ03_RS09670 (LTQ03_09670)	nadC	2088904..2089794 (-)	891	WP_017079961.1	carboxylating nicotinate-nucleotide diphosphorylase	-
LTQ03_RS09675 (LTQ03_09675)	ampD	2089878..2090477 (+)	600	WP_243583229.1	1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD	-
LTQ03_RS09680 (LTQ03_09680)	pdhR	2090958..2091719 (+)	762	WP_009847596.1	pyruvate dehydrogenase complex transcriptional repressor PdhR	-
LTQ03_RS09685 (LTQ03_09685)	aceE	2091782..2094445 (+)	2664	WP_243583230.1	pyruvate dehydrogenase (acetyl-transferring), homodimeric type	-
LTQ03_RS09690 (LTQ03_09690)	aceF	2094472..2096364 (+)	1893	WP_108123831.1	pyruvate dehydrogenase complex dihydrolipoyllysine-residue acetyltransferase	-
LTQ03_RS09695 (LTQ03_09695)	lpdA	2096607..2098034 (+)	1428	WP_004735450.1	dihydrolipoyl dehydrogenase	-

Sequence

Protein

• Length: 561 a.a.
• Molecular weight: 62010.62 Da
• Isoelectric Point: 4.8705

>NTDB_id=635669 LTQ03_RS09660 WP_243583227.1 2086502..2088187(-) (pilB) [Vibrio splendidus strain 1_C04a]
MLTNLPTVLRQAGLLSLTQEQAVAEHVQASGVSTPEALLVLDFFTGDSLANNIKTIFGLPLVQLASTDYETLCEQLGLRE
LITKYRAIPISVSSSTLTLASADPTDLQAEDDFRFATGLQIELVVANYSELEGAIRKLYGRSISGQDSKRKEITQDELAN
LVEISDDEITSIEDLSQDDSPVSRFINQILVDAARKGASDIHFEPYEENYRVRLRCDGILVEIQQPASHLSRRLSARLKI
LAKLDIAERRLPQDGRIKLRLNDELAIDMRVSTLPTLWGEKIVLRLLDSSAANLDIDKLGYSEDQKALYLNALKRPQGMI
LMTGPTGSGKTVSLYTGLRVLNTTERNISTAEDPVEINLCGINQVQVTPKIGFGFAEALRSFLRQDPDVVMVGEIRDLET
AEIAIKASQTGHLVLSTLHTNSAAETVTRLAHMGIEPFNLASSLSLIIAQRLARRLCDHCKAADDSPDIFLRHSIPNSQI
IYKANSQGCNECNQGYSGRVGIYEVMPFSDQLKNSLIDKPNALAIENLARREGMRTLQESGLDKLLEGTTSYQELQRVLY
L

Nucleotide

• Length: 1686 bp

>NTDB_id=635669 LTQ03_RS09660 WP_243583227.1 2086502..2088187(-) (pilB) [Vibrio splendidus strain 1_C04a]
ATGCTAACCAACCTCCCAACCGTTCTTCGTCAGGCAGGATTACTTAGCCTGACTCAAGAACAAGCCGTTGCGGAACATGT
ACAAGCTTCAGGTGTTTCTACGCCTGAAGCTTTGCTCGTTCTTGATTTCTTCACCGGCGATTCCTTAGCAAATAACATCA
AAACTATTTTCGGTTTACCGTTAGTACAACTAGCCAGTACTGACTACGAGACCTTGTGTGAGCAACTAGGGCTTCGCGAA
CTGATCACCAAGTATCGCGCTATTCCAATTTCAGTTTCTAGCTCAACTCTCACACTTGCCTCAGCCGACCCAACCGACTT
ACAAGCTGAAGACGACTTTCGATTCGCGACCGGATTACAGATTGAATTGGTTGTCGCTAATTACTCTGAATTAGAAGGCG
CAATACGAAAGCTGTATGGCCGTTCTATTTCCGGACAAGACTCCAAGCGTAAAGAGATCACTCAAGATGAGCTCGCTAAC
CTTGTTGAGATATCTGATGACGAGATAACTTCGATTGAAGACCTCAGCCAAGATGACTCCCCTGTCAGTCGCTTTATCAA
TCAGATTCTAGTCGATGCAGCACGTAAAGGCGCATCGGATATCCACTTCGAACCTTATGAAGAAAACTACCGCGTACGTC
TACGTTGCGATGGCATCCTTGTCGAAATTCAACAGCCCGCCTCTCATTTAAGTCGTCGCCTATCGGCTCGCTTGAAGATT
CTCGCCAAACTCGATATCGCCGAACGTCGCTTACCTCAAGATGGTCGTATCAAGTTACGTTTAAATGACGAACTCGCTAT
TGATATGCGTGTATCGACGCTGCCAACATTATGGGGAGAAAAGATCGTTCTTCGTCTTCTAGACAGCAGCGCTGCCAATC
TCGATATCGATAAGCTCGGTTACAGCGAGGATCAAAAAGCACTCTACCTCAATGCATTGAAACGCCCACAAGGGATGATC
TTAATGACCGGGCCAACGGGCAGCGGTAAAACGGTTTCTCTTTATACTGGCCTTCGAGTACTCAACACCACTGAACGCAA
TATATCAACAGCCGAAGACCCTGTTGAAATTAACCTATGTGGTATCAATCAGGTACAAGTGACGCCTAAGATCGGCTTTG
GTTTCGCTGAAGCGTTGCGATCATTTTTACGACAAGATCCCGATGTGGTAATGGTCGGTGAGATCCGCGATTTAGAAACC
GCAGAGATCGCGATCAAAGCATCGCAAACCGGACACCTCGTTCTTTCGACACTGCATACCAACTCCGCTGCAGAAACCGT
GACTCGACTCGCTCACATGGGAATTGAACCTTTTAATCTTGCTTCATCATTAAGTCTGATTATCGCCCAACGACTGGCAA
GACGATTGTGCGACCACTGTAAAGCCGCTGACGACTCACCTGATATATTTCTACGCCACTCTATTCCCAATAGCCAAATC
ATCTACAAAGCCAACTCACAAGGGTGTAATGAGTGTAATCAGGGATACTCGGGGCGAGTCGGTATCTATGAAGTCATGCC
GTTTAGCGACCAACTGAAAAATAGCCTGATAGACAAACCGAACGCGTTAGCCATTGAAAACTTGGCACGTCGAGAAGGCA
TGAGAACTTTACAAGAATCTGGGCTAGATAAACTGCTTGAAGGCACCACCAGCTATCAAGAGCTACAACGTGTTCTATAC
CTATAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (188-455)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (11-141)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Vibrio cholerae strain A1552	73.582	100	0.74
pilB	Vibrio campbellii strain DS40M4	70.641	100	0.708
pilB	Vibrio parahaemolyticus RIMD 2210633	70.285	100	0.704
pilB	Acinetobacter baumannii D1279779	50.674	92.513	0.469
pilB	Legionella pneumophila strain ERS1305867	50.196	90.909	0.456
pilB	Acinetobacter baylyi ADP1	49.133	92.513	0.455
pilF	Neisseria gonorrhoeae MS11	43.929	99.822	0.439
pilB/pilB1	Synechocystis sp. PCC 6803	35.462	100	0.376