Detailed information NTG0635026

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LSP22_RS15115
• Genome accession: NZ_CP089064
• Coordinates: 3258286..3259434 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain UNC_PaerCF35
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3253286..3264434

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LSP22_RS15090 (LSP22_15095)	-	3254437..3254733 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
LSP22_RS15095 (LSP22_15100)	-	3254730..3255323 (-)	594	WP_003084544.1	YggT family protein	-
LSP22_RS15100 (LSP22_15105)	proC	3255334..3256155 (-)	822	WP_003084546.1	pyrroline-5-carboxylate reductase	-
LSP22_RS15105 (LSP22_15110)	-	3256167..3256859 (-)	693	WP_023464234.1	YggS family pyridoxal phosphate-dependent enzyme	-
LSP22_RS15110 (LSP22_15115)	pilT	3257074..3258108 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
LSP22_RS15115 (LSP22_15120)	pilU	3258286..3259434 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
LSP22_RS15120 (LSP22_15125)	czcD	3259442..3260341 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
LSP22_RS15125 (LSP22_15130)	-	3260495..3260899 (+)	405	WP_003084560.1	NINE protein	-
LSP22_RS15130 (LSP22_15135)	-	3261142..3262515 (+)	1374	WP_003100955.1	pyridoxal-phosphate dependent enzyme	-
LSP22_RS15135 (LSP22_15140)	-	3262512..3263696 (+)	1185	WP_003100953.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=635026 LSP22_RS15115 WP_003100959.1 3258286..3259434(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF35]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=635026 LSP22_RS15115 WP_003100959.1 3258286..3259434(+) (pilU) [Pseudomonas aeruginosa strain UNC_PaerCF35]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382