Detailed information NTG0633984

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: LRK54_RS03945
• Genome accession: NZ_CP088922
• Coordinates: 831208..832932 (+)
• Length: 574 a.a.
• NCBI ID: WP_027490751.1
• Uniprot ID: -
• Organism: Rhodanobacter denitrificans strain FW104-10B01
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	817384..834893	831208..832932	within	0

Gene organization within MGE regions

Location: 817384..834893

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LRK54_RS03885 (LRK54_03885)	-	817384..819396 (+)	2013	WP_027490744.1	hypothetical protein	-
LRK54_RS03890 (LRK54_03890)	-	819396..821360 (+)	1965	WP_027490745.1	hypothetical protein	-
LRK54_RS03895 (LRK54_03895)	-	821760..823007 (+)	1248	WP_027490746.1	lipopolysaccharide biosynthesis protein	-
LRK54_RS03900 (LRK54_03900)	-	823504..824361 (+)	858	WP_196802027.1	class I SAM-dependent methyltransferase	-
LRK54_RS03905 (LRK54_03905)	-	824358..825365 (+)	1008	WP_196802028.1	glycosyltransferase	-
LRK54_RS03910 (LRK54_03910)	-	825425..826324 (-)	900	WP_196802029.1	glycosyltransferase	-
LRK54_RS03915 (LRK54_03915)	-	826308..827258 (-)	951	WP_027490747.1	glycosyltransferase family 2 protein	-
LRK54_RS03920 (LRK54_03920)	-	827255..827551 (-)	297	WP_027490748.1	hypothetical protein	-
LRK54_RS03925 (LRK54_03925)	-	827548..828801 (-)	1254	WP_027490749.1	glycosyltransferase family 4 protein	-
LRK54_RS03930 (LRK54_03930)	-	828794..829648 (-)	855	WP_027490750.1	glycosyltransferase family 2 protein	-
LRK54_RS03935 (LRK54_03935)	-	829940..830335 (-)	396	WP_007513922.1	type II toxin-antitoxin system VapC family toxin	-
LRK54_RS03940 (LRK54_03940)	-	830332..830574 (-)	243	WP_007513924.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
LRK54_RS03945 (LRK54_03945)	pilB	831208..832932 (+)	1725	WP_027490751.1	type IV-A pilus assembly ATPase PilB	Machinery gene
LRK54_RS03950 (LRK54_03950)	pilC	833075..834346 (+)	1272	WP_027490752.1	type II secretion system F family protein	Machinery gene
LRK54_RS03955 (LRK54_03955)	-	834495..834893 (+)	399	WP_008438584.1	endonuclease domain-containing protein	-

Sequence

Protein

• Length: 574 a.a.
• Molecular weight: 62713.47 Da
• Isoelectric Point: 6.3337

>NTDB_id=633984 LRK54_RS03945 WP_027490751.1 831208..832932(+) (pilB) [Rhodanobacter denitrificans strain FW104-10B01]
MSSQLQPSLAGLSGMARRLVSEGVMPEDVVRKAMADSSQQKATLGAWLLDRSLVDSAELTRIASAEFGMPMMDVAALNPA
TMPLDLISEALITKHQALPLFRRGKRLYVGIADPMQSHALDEIKFHSNCMVEPILVERGSLQRVIETLLNNMRNTMPEMG
GGELDELDIEGGDDEGESSSGVDANANDDAPVVKFVNKILVDAIRRGASDIHFEPFETQYRVRLRMDGMLKAVANPPMKM
ANRISSRIKVMAQLDIAEKRVPQDGRIKLNLSKTRAVDFRVSTLPTLFGEKIVLRILDGSSARIGIEKLGYEPNQQKLYV
DAIHKPYGMVLVTGPTGSGKTVSLYTGLNMLNTAERNISTVEDPVEIRVEGINQVQQNVKRGMTFASALRSFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAAQTISRLMNMGIAPYNITSSVTLIIAQRLARRLHDCKKPIELPPKVL
LDAGFTQADIDAGLTIYEAGGCDGCNEGYKGRVGIYQVMPMLEDIQKIILQGGNALQIAEVAKAAGINDLRASALLKVKQ
GLTSLTEIDRVTKE

Nucleotide

• Length: 1725 bp

>NTDB_id=633984 LRK54_RS03945 WP_027490751.1 831208..832932(+) (pilB) [Rhodanobacter denitrificans strain FW104-10B01]
ATGTCATCTCAATTACAGCCGTCGCTTGCGGGCTTGTCCGGCATGGCCCGCCGGTTGGTCTCCGAAGGCGTGATGCCGGA
GGACGTGGTACGCAAGGCCATGGCCGACAGCAGCCAGCAGAAGGCCACGCTGGGCGCCTGGCTGCTGGACCGCAGCCTGG
TCGACAGCGCCGAACTGACCCGGATCGCCTCGGCCGAATTCGGCATGCCGATGATGGACGTGGCCGCGCTGAACCCGGCC
ACGATGCCGCTGGACCTGATCAGCGAGGCGCTGATCACCAAGCACCAGGCGCTGCCGCTGTTCCGCCGCGGCAAGCGGCT
GTACGTGGGCATCGCCGACCCGATGCAGTCGCATGCGCTGGACGAGATCAAGTTCCACTCCAACTGCATGGTGGAGCCGA
TCCTGGTGGAGCGTGGCTCGCTGCAGCGAGTGATCGAAACCCTGCTCAACAACATGCGCAACACCATGCCCGAGATGGGC
GGGGGCGAGCTGGACGAGCTGGACATCGAGGGCGGCGACGACGAGGGCGAGTCGAGCAGCGGCGTCGATGCGAACGCGAA
CGACGACGCGCCGGTGGTGAAGTTCGTCAACAAGATCCTGGTCGACGCGATCCGCCGCGGCGCCTCGGACATCCATTTCG
AGCCGTTCGAGACGCAGTACCGCGTGCGCCTGCGCATGGACGGCATGCTGAAGGCGGTGGCCAACCCGCCGATGAAGATG
GCCAACCGCATCTCCTCGCGCATCAAGGTGATGGCCCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCAT
CAAGCTCAACCTGTCCAAGACCCGCGCGGTCGACTTTCGCGTGAGCACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGC
TGCGCATCCTGGACGGCTCCTCGGCCAGGATCGGCATCGAGAAGCTGGGCTACGAGCCGAACCAGCAGAAGCTCTACGTC
GATGCGATCCATAAGCCGTACGGCATGGTGCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGG
CCTGAACATGCTCAACACCGCCGAGCGCAACATCTCGACGGTGGAAGACCCGGTGGAAATCCGCGTCGAGGGCATCAACC
AGGTGCAGCAGAACGTGAAGCGCGGCATGACCTTTGCCAGCGCGCTGCGCTCGTTCCTGCGCCAGGACCCGGACGTGATC
ATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGAGATCGCGATCAAGGCGGCGCAGACCGGTCACATGGTGCTGTCCAC
CCTGCACACCAACGACGCGGCGCAGACCATCTCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCTCGTCGG
TGACCCTGATCATCGCCCAGCGCCTGGCGCGGCGCCTGCACGACTGCAAGAAGCCGATCGAGCTGCCGCCCAAGGTGCTG
CTGGACGCCGGCTTCACCCAGGCCGACATCGACGCGGGGCTGACCATCTATGAAGCCGGCGGCTGCGACGGCTGCAACGA
GGGCTACAAGGGCCGTGTCGGCATCTACCAGGTGATGCCGATGCTGGAGGATATCCAGAAGATCATTCTGCAAGGCGGCA
ACGCGCTGCAGATCGCCGAGGTGGCCAAGGCCGCCGGCATCAACGACCTGCGCGCGTCGGCCCTGCTGAAGGTGAAGCAG
GGGCTGACCAGCCTGACCGAGATCGACCGCGTCACCAAGGAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (198-465)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-152)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.044	98.432	0.542
pilB	Acinetobacter baumannii D1279779	53.427	99.129	0.53
pilB	Legionella pneumophila strain ERS1305867	49.562	99.477	0.493
pilB	Vibrio cholerae strain A1552	51.036	92.509	0.472
pilB	Vibrio parahaemolyticus RIMD 2210633	49.906	92.509	0.462
pilB	Vibrio campbellii strain DS40M4	49.906	92.509	0.462
pilF	Neisseria gonorrhoeae MS11	46.869	97.387	0.456
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	37.268	100	0.385
pilF	Thermus thermophilus HB27	38.378	96.69	0.371