Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LPA45_RS15190 Genome accession   NZ_CP088083
Coordinates   3243408..3245186 (+) Length   592 a.a.
NCBI ID   WP_367394624.1    Uniprot ID   -
Organism   Cupriavidus sp. Agwp_2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3238408..3250186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LPA45_RS15155 (LPA45_15160) proB 3238558..3239676 (-) 1119 WP_116318211.1 glutamate 5-kinase -
  LPA45_RS15160 (LPA45_15165) obgE 3239722..3240819 (-) 1098 WP_116318212.1 GTPase ObgE -
  LPA45_RS15165 (LPA45_15170) rpmA 3240981..3241241 (-) 261 WP_010814747.1 50S ribosomal protein L27 -
  LPA45_RS15170 (LPA45_15175) rplU 3241281..3241592 (-) 312 WP_006576525.1 50S ribosomal protein L21 -
  LPA45_RS15175 (LPA45_15180) - 3241789..3242070 (-) 282 WP_116318213.1 hypothetical protein -
  LPA45_RS15180 (LPA45_15185) ispB 3242069..3242998 (+) 930 WP_367394623.1 octaprenyl diphosphate synthase -
  LPA45_RS15190 (LPA45_15195) pilF 3243408..3245186 (+) 1779 WP_367394624.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  LPA45_RS15195 (LPA45_15200) - 3245278..3246549 (+) 1272 WP_367394625.1 type II secretion system F family protein -
  LPA45_RS15200 (LPA45_15205) - 3246553..3247455 (+) 903 WP_367394626.1 A24 family peptidase -
  LPA45_RS15205 (LPA45_15210) coaE 3247507..3248145 (+) 639 WP_367394627.1 dephospho-CoA kinase -
  LPA45_RS15210 (LPA45_15215) zapD 3248383..3249141 (+) 759 WP_116318219.1 cell division protein ZapD -
  LPA45_RS15215 (LPA45_15220) - 3249159..3249350 (+) 192 WP_116318220.1 DNA gyrase inhibitor YacG -
  LPA45_RS15220 (LPA45_15225) - 3249480..3249674 (-) 195 Protein_2984 hypothetical protein -
  LPA45_RS15225 (LPA45_15230) - 3249694..3250131 (-) 438 WP_116318221.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 592 a.a.        Molecular weight: 65495.31 Da        Isoelectric Point: 8.0651

>NTDB_id=632627 LPA45_RS15190 WP_367394624.1 3243408..3245186(+) (pilF) [Cupriavidus sp. Agwp_2]
MTLGLALTQSRRIAPALLAQLEQAAREKQSQLIDEIVGSGTMSAHDLALFAADKYQLPLLDLNQYNLTKVPPGLAGNREF
HAHRLLPLGRRENRLVLAMSDPSNQAGLDAIKEKYKLPVEAVVVEHDKLMKHVRSAGEALGTLKNISPVQTERKMIEYDP
VAAASAQRNRTAADAIDDAPVVRFLQKLLTEAFHRGASDLHFEPFETFYRIRFRVDGVLQEVARPPLDIRDKIATRIKVL
SRLDISEKRVPQDGRMKLLIALPKDNKDNKDNKDSKDAKDPKDKDVKETVEKAVDFRVSTLPTLFGEKIVMRILESSSDK
LDIDQLGYEPQQKALLLDVIKRPYGMVLVTGPTGSGKTVSLYTFLNLLNQGDINISTAEDPAEIQLPGINQVNVNDKAGL
TFAAALRSFLRQDPDIIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLA
RRLCTCKRPGELPRETLRDAGFREQDLDGSWQPYHPVGCERCNGSGYKGRCGIYQVMPITEAMQQIILSHGTALQIAEQA
RKDGVLSLREAGLLKVRQGVTSLEEVLATTNT

Nucleotide


Download         Length: 1779 bp        

>NTDB_id=632627 LPA45_RS15190 WP_367394624.1 3243408..3245186(+) (pilF) [Cupriavidus sp. Agwp_2]
ATGACACTCGGTCTTGCCCTGACCCAGAGCCGGCGTATCGCGCCCGCCCTGCTTGCTCAGCTGGAGCAGGCCGCCCGTGA
AAAGCAGTCCCAGTTGATCGACGAGATCGTCGGTAGCGGCACCATGAGCGCGCACGACCTCGCGCTGTTCGCGGCGGACA
AATACCAGCTGCCGCTGCTGGACCTGAACCAGTACAACCTGACCAAGGTGCCGCCGGGCTTGGCGGGCAACCGGGAATTC
CATGCGCACCGCCTGCTGCCGCTGGGCCGGCGCGAGAACCGGCTGGTGCTGGCAATGTCCGATCCGTCCAACCAGGCCGG
GCTGGACGCGATCAAGGAAAAGTACAAGCTGCCGGTCGAAGCGGTGGTGGTCGAGCACGACAAGCTGATGAAGCACGTGC
GCTCCGCCGGCGAGGCGCTGGGCACACTGAAGAACATCTCGCCGGTGCAGACCGAGCGCAAGATGATCGAGTACGACCCG
GTGGCGGCCGCCAGCGCGCAGCGCAACCGCACTGCCGCCGATGCCATCGATGACGCCCCCGTGGTGCGCTTCCTGCAGAA
GCTGCTGACCGAAGCCTTCCACCGCGGCGCGTCCGACCTGCATTTCGAGCCGTTCGAGACCTTCTACCGGATCCGCTTCC
GCGTGGACGGCGTGCTGCAGGAGGTGGCAAGGCCGCCGCTGGATATCCGCGACAAGATCGCCACGCGCATCAAGGTGCTG
TCCCGGCTGGATATTTCTGAAAAGCGCGTGCCGCAGGACGGGCGCATGAAGCTGCTGATCGCGCTGCCGAAGGACAACAA
GGACAACAAGGACAACAAGGACAGCAAGGACGCGAAGGATCCCAAGGACAAGGACGTCAAGGAAACGGTCGAGAAGGCCG
TCGATTTCCGCGTCTCGACGCTGCCGACGCTGTTCGGCGAGAAGATCGTGATGCGGATCCTGGAATCGTCGAGCGACAAG
CTCGACATCGACCAGCTCGGCTACGAACCGCAGCAGAAGGCGCTGCTGCTGGACGTGATCAAGCGCCCGTACGGCATGGT
ACTGGTGACAGGCCCCACCGGCAGCGGCAAGACGGTGTCGCTGTACACCTTCCTGAACCTGCTGAACCAGGGCGACATCA
ATATCTCGACCGCCGAGGATCCGGCTGAAATCCAGCTGCCCGGCATCAACCAGGTCAACGTCAACGACAAGGCCGGCCTG
ACCTTCGCCGCGGCGCTGCGCTCGTTCCTGCGGCAGGATCCGGACATCATCATGGTCGGCGAAATCCGTGACCTGGAAAC
CGCCGATATCTCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTCTCGACGCTGCACACCAACGACGCGCCCACCACGC
TGACGCGGCTGATGAACATGGGCGTGGCGCCGTTCAATATCGCCTCCAGCGTGCTGATGATCACCGCGCAGCGGCTGGCG
CGGCGGCTGTGCACCTGCAAGCGCCCCGGCGAGCTGCCGCGCGAGACGCTGCGGGATGCGGGCTTCCGCGAACAGGACCT
GGACGGCAGCTGGCAGCCCTACCACCCGGTCGGTTGCGAGCGCTGCAACGGCAGCGGCTACAAAGGCCGCTGCGGCATTT
ACCAGGTCATGCCGATCACCGAGGCGATGCAGCAGATCATCCTGTCGCACGGCACCGCCCTGCAGATCGCCGAGCAGGCG
CGCAAGGACGGCGTGCTATCGTTGCGCGAGGCTGGGCTGCTGAAGGTCAGGCAGGGCGTAACATCACTCGAAGAGGTGCT
GGCGACCACGAATACGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-134)

Type II/IV secretion system protein (T2SSE)

(275-482)

Type II/IV secretion system protein (T2SSE)

(187-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

50.251

100

0.507
  pilB Acinetobacter baumannii D1279779

50.426

99.155

0.5
  pilB Acinetobacter baylyi ADP1

50.176

95.946

0.481
  pilB Legionella pneumophila strain ERS1305867

48.858

96.115

0.47
  pilB Vibrio cholerae strain A1552

43.98

100

0.444
  pilB Vibrio parahaemolyticus RIMD 2210633

44.484

94.932

0.422
  pilB Vibrio campbellii strain DS40M4

44.544

94.426

0.421
  pilF Thermus thermophilus HB27

38.475

97.466

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.408

97.635

0.375