NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	N5914_RS02215	Genome accession	NZ_CP104665
Coordinates	475027..476151 (+)	Length	374 a.a.
NCBI ID	WP_000228569.1	Uniprot ID	-
Organism	Escherichia coli strain 2021CK-00607
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 470027..481151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N5914_RS02190 (N5914_02190)	mscL	470153..470563 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
N5914_RS02195 (N5914_02195)	trkA	470693..472069 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
N5914_RS02200 (N5914_02200)	rsmB	472091..473380 (-)	1290	WP_001329571.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
N5914_RS02205 (N5914_02205)	fmt	473426..474373 (-)	948	WP_022646187.1	methionyl-tRNA formyltransferase	-
N5914_RS02210 (N5914_02210)	def	474388..474897 (-)	510	WP_000114986.1	peptide deformylase	-
N5914_RS02215 (N5914_02215)	dprA	475027..476151 (+)	1125	WP_000228569.1	DNA-protecting protein DprA	Machinery gene
N5914_RS02220 (N5914_02220)	smg	476123..476596 (+)	474	WP_000460672.1	DUF494 family protein Smg	-
N5914_RS02225 (N5914_02225)	yrdD	476625..477167 (+)	543	WP_001129718.1	type I DNA topoisomerase	-
N5914_RS02230 (N5914_02230)	tsaC	477172..477744 (+)	573	WP_001353637.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
N5914_RS02235 (N5914_02235)	aroE	477749..478567 (+)	819	WP_000432883.1	shikimate dehydrogenase	-
N5914_RS02240 (N5914_02240)	yrdB	478564..478821 (+)	258	WP_001070584.1	DUF1488 domain-containing protein	-
N5914_RS02245 (N5914_02245)	yrdA	478797..479351 (-)	555	WP_022646186.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40916.82 Da Isoelectric Point: 6.0395

                                >NTDB_id=632260 N5914_RS02215 WP_000228569.1 475027..476151(+) (dprA) [Escherichia coli strain 2021CK-00607]

MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLISADSE

FYPPQLLATTDYPGTLFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=632260 N5914_RS02215 WP_000228569.1 475027..476151(+) (dprA) [Escherichia coli strain 2021CK-00607]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCGTCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCTCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCACACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACTATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAACAGGGTGGTGCTCTCGTCTCGGAATTTCCCCTTGATGTTCCTCCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCGGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTAACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	47.826	98.396	0.471
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.032	82.888	0.406
dprA	Vibrio cholerae strain A1552	49.032	82.888	0.406
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	43.939	88.235	0.388

Detailed information NTG0632260