Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   N5856_RS18350 Genome accession   NZ_CP104613
Coordinates   3796565..3798058 (-) Length   497 a.a.
NCBI ID   WP_094318010.1    Uniprot ID   -
Organism   Escherichia coli strain AHM8C212RI     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3791565..3803058
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5856_RS18320 (N5856_18255) gspK 3792359..3793336 (-) 978 WP_094318014.1 type II secretion system minor pseudopilin GspK -
  N5856_RS18325 (N5856_18260) gspJ 3793339..3793938 (-) 600 WP_094318013.1 type II secretion system minor pseudopilin GspJ -
  N5856_RS18330 (N5856_18265) gspI 3793935..3794306 (-) 372 WP_097702256.1 type II secretion system minor pseudopilin GspI -
  N5856_RS18335 (N5856_18270) gspH 3794303..3794866 (-) 564 WP_094318012.1 type II secretion system minor pseudopilin GspH -
  N5856_RS18340 (N5856_18275) gspG 3794870..3795325 (-) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  N5856_RS18345 (N5856_18280) gspF 3795342..3796565 (-) 1224 WP_094318011.1 type II secretion system inner membrane protein GspF -
  N5856_RS18350 (N5856_18285) pilB/pilB1 3796565..3798058 (-) 1494 WP_094318010.1 type II secretion system ATPase GspE Machinery gene
  N5856_RS18355 (N5856_18290) gspD 3798058..3800118 (-) 2061 WP_000498824.1 type II secretion system secretin GspD -
  N5856_RS18360 (N5856_18295) gspC 3800148..3801107 (-) 960 WP_077039129.1 type II secretion system protein GspC -
  N5856_RS18365 (N5856_18300) gspS2 3801125..3801535 (-) 411 WP_001345954.1 type II secretion system pilot lipoprotein GspS-beta -
  N5856_RS18370 (N5856_18305) pilD 3801601..3802410 (-) 810 WP_077039130.1 prepilin peptidase PppA Machinery gene

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55208.34 Da        Isoelectric Point: 6.0390

>NTDB_id=631745 N5856_RS18350 WP_094318010.1 3796565..3798058(-) (pilB/pilB1) [Escherichia coli strain AHM8C212RI]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSTGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=631745 N5856_RS18350 WP_094318010.1 3796565..3798058(-) (pilB/pilB1) [Escherichia coli strain AHM8C212RI]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAGCTGATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCGGAAGAACTGCCAGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGCTGCGT
CCGGGGCGCAAACTGGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGTTGCTGGGCGGCCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAGCGTCTGGGGCTTAGCCAGCAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGCAACATTCTCACGGTTGAAGATCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTTGCCCGTGGGCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTCCAGGCTTCACTTACCGGACACCT
GGTCCTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACTGGCGTGATGGCGCAGCGACTGGTTCGTACGCTGTGCCCCGACTGCCGCCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGACGCCCGTACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTACGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTCACGATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baylyi ADP1

46.154

78.471

0.362
  pilF Thermus thermophilus HB27

45

80.483

0.362