Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LO767_RS00750 Genome accession   NZ_CP087705
Coordinates   155508..156572 (+) Length   354 a.a.
NCBI ID   WP_228245562.1    Uniprot ID   -
Organism   Halopseudomonas aestusnigri strain GOM5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 150508..161572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LO767_RS00740 (LO767_00740) - 152019..152969 (+) 951 WP_228247636.1 LysR family transcriptional regulator -
  LO767_RS00745 (LO767_00745) - 152924..155323 (-) 2400 WP_233048948.1 penicillin-binding protein 1A -
  LO767_RS00750 (LO767_00750) pilM 155508..156572 (+) 1065 WP_228245562.1 pilus assembly protein PilM Machinery gene
  LO767_RS00755 (LO767_00755) - 156572..157150 (+) 579 WP_233048949.1 PilN domain-containing protein -
  LO767_RS00760 (LO767_00760) - 157147..157770 (+) 624 WP_088277117.1 type 4a pilus biogenesis protein PilO -
  LO767_RS00765 (LO767_00765) pilP 157785..158297 (+) 513 WP_233049927.1 type 4a pilus biogenesis lipoprotein PilP -
  LO767_RS00770 (LO767_00770) pilQ 158354..160426 (+) 2073 WP_233048950.1 type IV pilus secretin PilQ Machinery gene
  LO767_RS00775 (LO767_00775) aroK 160432..160941 (+) 510 WP_233048951.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37972.49 Da        Isoelectric Point: 4.3061

>NTDB_id=631077 LO767_RS00750 WP_228245562.1 155508..156572(+) (pilM) [Halopseudomonas aestusnigri strain GOM5]
MLGLFKKKANTLLGIDISSTTVKLLELSRSGGRYKVEAYAVEPLPPASVVEKNIVELEGVGQALEKVLARSKSSLRQAAV
AVAGSAVITKVIEMDAGLDPDEMEEQIKIEADQYIPYPLDEVAIDFEVQGVSPRNPAREEVLLAACRRENVDIREAALAL
AGLTAKIVEVEAYAMERACELVIGQLDAGNDDLTIAVIDIGATMMTLSVLSGGRTIYTREQLFGGKQLTDEIQRRYGLSQ
DEAGLAKKQGGLPDDYEVEVLGPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDQLVQQKIGTTTLVANP
FANMTLSNKVDAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=631077 LO767_RS00750 WP_228245562.1 155508..156572(+) (pilM) [Halopseudomonas aestusnigri strain GOM5]
GTGCTAGGGCTCTTCAAGAAAAAAGCGAATACGCTGCTGGGCATCGACATCAGCTCCACCACCGTGAAGCTGCTCGAACT
CAGCCGCTCCGGTGGCCGCTACAAGGTCGAGGCCTACGCGGTCGAACCTCTCCCTCCCGCTTCGGTAGTGGAGAAGAACA
TTGTCGAACTCGAAGGCGTCGGACAGGCGCTGGAGAAGGTTCTGGCGCGATCGAAATCGTCGCTCAGGCAGGCCGCAGTG
GCGGTAGCCGGATCCGCGGTGATCACCAAGGTGATCGAGATGGATGCCGGGCTTGATCCTGACGAGATGGAAGAACAGAT
CAAGATCGAAGCCGATCAGTACATCCCCTACCCGCTGGACGAGGTTGCCATAGACTTCGAAGTCCAGGGCGTTTCCCCCC
GAAATCCCGCCAGAGAAGAAGTGTTGCTCGCTGCCTGTCGCCGTGAAAACGTCGATATCCGCGAAGCCGCACTGGCACTT
GCCGGTCTGACCGCCAAGATTGTCGAGGTCGAGGCTTACGCGATGGAGCGTGCCTGCGAGCTTGTTATCGGGCAGTTGGA
TGCGGGCAACGATGACCTGACCATCGCGGTGATCGACATCGGTGCAACCATGATGACCCTGAGTGTGCTCAGTGGTGGTC
GCACCATTTATACCCGCGAGCAGCTGTTTGGCGGCAAGCAACTGACGGATGAGATTCAGCGCCGTTACGGCTTGTCCCAG
GACGAGGCCGGGCTGGCCAAGAAGCAGGGCGGTCTTCCCGACGATTACGAAGTCGAGGTGCTGGGTCCCTTCAAGGATGC
AGTGGTGCAGCAGGTATCTCGATCGCTGCAGTTCTTCTTTGCCGGCGGGCAATACAACGACGTCGACTACATCTTGCTCG
CCGGTGGTACCGCTTCGATTCCAGGGCTGGATCAGTTGGTACAGCAGAAAATTGGTACCACCACTCTGGTTGCCAACCCC
TTTGCAAACATGACCCTGTCAAACAAGGTTGATGCGGTTGCCCTGAGCAATGACGCGCCCGCCCTGATGATTGCGTGTGG
TCTGGCAATGAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.197

100

0.463