Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NQS35_RS14330 Genome accession   NZ_CP104494
Coordinates   2998596..3000734 (-) Length   712 a.a.
NCBI ID   WP_276145546.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain Cw717     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2993596..3005734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NQS35_RS14305 (NQS35_14265) - 2993781..2994506 (-) 726 WP_069079150.1 transposase -
  NQS35_RS14310 (NQS35_14270) - 2994772..2995446 (+) 675 WP_016727683.1 OmpW/AlkL family protein -
  NQS35_RS14315 (NQS35_14275) - 2995596..2996759 (-) 1164 WP_069079151.1 deoxyguanosinetriphosphate triphosphohydrolase -
  NQS35_RS14320 (NQS35_14280) aroB 2996779..2997885 (-) 1107 WP_019718894.1 3-dehydroquinate synthase -
  NQS35_RS14325 (NQS35_14285) - 2997882..2998454 (-) 573 WP_011002875.1 shikimate kinase -
  NQS35_RS14330 (NQS35_14290) pilQ 2998596..3000734 (-) 2139 WP_276145546.1 temperature dependent type IV pilus secretin PilQ Machinery gene
  NQS35_RS14335 (NQS35_14295) - 3000737..3001282 (-) 546 WP_011002877.1 pilus assembly protein PilP -
  NQS35_RS14340 (NQS35_14300) - 3001279..3001938 (-) 660 WP_020832821.1 type 4a pilus biogenesis protein PilO -
  NQS35_RS14345 (NQS35_14305) - 3001941..3002573 (-) 633 WP_011002879.1 PilN domain-containing protein -
  NQS35_RS14350 (NQS35_14310) pilM 3002587..3003657 (-) 1071 WP_011002880.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 712 a.a.        Molecular weight: 76349.44 Da        Isoelectric Point: 9.8259

>NTDB_id=629759 NQS35_RS14330 WP_276145546.1 2998596..3000734(-) (pilQ) [Ralstonia pseudosolanacearum strain Cw717]
MRRVKGGLVQAVRVARGGAVAWLSLCMLMVGGQALAQQAITAPASNAVERVEQASTGESTVVTVTLKDAPAQKPVEFSTQ
QPARIAIDFFGTAFAQGRANYQYGGKLLRAASVVQIGDRTRVVLDLARQSQYKSEIRGNQYVLTLGAAPTASATPVPTFA
APAPTAGVERPSVRNIDFRRGEELAGRVVVDLSTSNSAINIAQQGQNLVVDFAGATLPQSLRRRFDVSDFGTPVQAMRAT
DNGTGARLVIEPRGNWQYSSYQTDTQFVVEVRPTKEDPNKLISGPGYRGERMSLNFQNIDIRSLLQVFADFTNLNIVTSD
SVTGTLSLRLKDVPWDQALQIVLDSKGLASRRNGNVLWVAPRNELATKEKAELESQQQVTELEPLRSQVFRLNYQRADDV
RNMLLGTGGTAGGGAAASRILSKRGSLTSDARTNQLFVSDIPSKLEEVQAFLLKIDIPVRQVMIEARIVEADDTFSRNLG
AKLGFAAKTNGAGYGNTYSNVVSPVTKDATWDNSAAVSLPANGINGVNAASVAVSLFNAGAGRFLALELSALEADGRGKI
ISSPRVVTADNIKALIEQGTEVPYQQATSSGATSVSFKKANLKLEVTPKITPDGNVFLDVDVNKDSLGTQTTNGFAINTK
HVQTQVLVENGGTVVIGGIYTQNERTDVNKVPLLGDIPVLGNLFKSTAKTNDRTELLVFLTPRVLSDQLSLK

Nucleotide


Download         Length: 2139 bp        

>NTDB_id=629759 NQS35_RS14330 WP_276145546.1 2998596..3000734(-) (pilQ) [Ralstonia pseudosolanacearum strain Cw717]
ATGCGTCGCGTGAAGGGCGGCCTGGTGCAGGCCGTTCGAGTGGCCCGGGGGGGCGCAGTGGCATGGCTTTCGCTGTGTAT
GCTGATGGTGGGTGGACAGGCGTTGGCCCAGCAGGCAATCACGGCGCCGGCAAGCAATGCCGTCGAACGCGTGGAACAAG
CCTCGACAGGCGAATCCACCGTGGTGACGGTGACGCTGAAGGACGCGCCCGCCCAGAAGCCGGTGGAATTCAGCACGCAG
CAGCCTGCGCGCATCGCCATCGACTTCTTCGGCACGGCATTCGCCCAGGGGCGTGCCAACTATCAATATGGCGGCAAGCT
GCTGCGTGCGGCCAGCGTGGTGCAGATCGGTGACCGCACCCGCGTCGTGCTGGATCTGGCGCGCCAGTCGCAGTACAAGT
CCGAGATCCGGGGCAACCAGTACGTGCTGACGCTGGGCGCCGCGCCGACGGCGTCGGCCACCCCGGTGCCGACCTTCGCC
GCGCCCGCGCCGACGGCCGGTGTGGAGCGGCCGTCCGTGCGCAACATCGACTTCCGCCGTGGCGAAGAACTGGCCGGCCG
CGTGGTGGTCGACCTGTCGACGTCCAACTCCGCGATCAACATCGCTCAGCAGGGGCAAAACCTGGTGGTCGATTTCGCCG
GTGCCACGCTGCCGCAATCGCTGCGTCGCCGCTTTGACGTGAGCGACTTTGGCACGCCGGTGCAGGCCATGCGCGCCACC
GACAACGGCACGGGCGCTCGCCTGGTCATCGAGCCGCGCGGCAACTGGCAGTACAGCTCGTACCAGACCGACACGCAGTT
CGTGGTGGAAGTCCGCCCGACCAAGGAGGACCCGAACAAGCTGATCTCCGGTCCGGGCTATCGCGGGGAGCGCATGTCGC
TGAACTTCCAGAACATCGACATTCGTTCGCTGCTGCAGGTCTTTGCCGACTTCACCAACCTGAACATCGTCACCAGCGAC
AGCGTGACGGGGACCCTGTCGCTGCGTCTGAAGGACGTGCCGTGGGATCAGGCGCTGCAGATCGTGCTGGATTCCAAGGG
CCTGGCCTCGCGCCGCAACGGCAACGTGCTGTGGGTGGCGCCGCGCAACGAACTGGCGACCAAGGAGAAGGCTGAGCTGG
AGTCGCAGCAACAGGTGACCGAGCTGGAGCCGCTGCGCAGCCAGGTGTTCCGTCTGAATTACCAGCGTGCTGACGACGTG
CGCAACATGCTGCTGGGTACCGGAGGAACGGCGGGCGGTGGTGCTGCGGCGTCGCGCATCTTGTCCAAGCGTGGCTCGCT
GACGTCGGATGCCCGTACCAACCAGCTGTTCGTCTCGGATATCCCGAGCAAGCTGGAGGAGGTGCAGGCCTTCCTGCTGA
AGATCGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCGATGACACGTTCAGCCGCAATCTTGGG
GCAAAGCTGGGCTTCGCGGCCAAGACCAATGGTGCCGGGTATGGCAACACCTACAGCAACGTGGTGTCGCCGGTCACGAA
AGACGCCACTTGGGATAACAGCGCGGCCGTCAGCCTGCCCGCCAATGGCATCAACGGTGTGAACGCCGCCAGTGTGGCAG
TGAGCTTGTTCAACGCCGGTGCCGGCCGTTTCCTGGCGCTCGAACTGTCGGCGCTGGAAGCCGATGGCCGCGGCAAGATC
ATCTCCAGCCCGCGTGTGGTCACCGCTGACAACATCAAGGCGCTGATCGAGCAGGGTACCGAAGTGCCTTACCAGCAGGC
GACCTCGTCGGGTGCGACGTCGGTTTCGTTCAAAAAGGCCAACCTGAAATTGGAGGTGACGCCGAAGATTACGCCGGACG
GCAACGTGTTCCTGGACGTGGACGTCAACAAGGACAGCCTGGGCACGCAGACCACCAACGGCTTCGCCATCAACACCAAG
CACGTGCAGACCCAGGTGCTGGTCGAGAACGGCGGCACGGTTGTGATTGGCGGCATCTACACGCAGAACGAACGGACCGA
TGTGAACAAGGTCCCGCTGCTGGGTGACATCCCGGTGCTGGGCAACCTGTTCAAGAGCACGGCCAAGACCAACGACCGGA
CGGAACTGCTGGTGTTCCTGACGCCGCGCGTGCTGTCCGACCAACTGTCGCTGAAGTAA

Domains

Predicted by InterProScan.

AMIN domain (AMIN)

(61-143)

AMIN domain (AMIN)

(195-270)

Secretin and TonB N terminus short domain (STN)

(316-361)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-461)

Bacterial type II and III secretion system protein (Secretin)

(551-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

41.467

93.82

0.389
  pilQ Neisseria gonorrhoeae strain FA1090

36.401

100

0.372
  comQ/pilQ Acinetobacter baylyi ADP1

38.15

97.191

0.371
  pilQ Legionella pneumophila strain Lp02

39.269

92.275

0.362
  pilQ Legionella pneumophila strain ERS1305867

39.269

92.275

0.362