Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   N4S17_RS11300 Genome accession   NZ_CP104472
Coordinates   2320609..2321733 (-) Length   374 a.a.
NCBI ID   WP_000228519.1    Uniprot ID   -
Organism   Escherichia coli strain UC4224     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2315609..2326733
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4S17_RS11270 (N4S17_11270) yrdA 2317409..2317963 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  N4S17_RS11275 (N4S17_11275) yrdB 2317939..2318196 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  N4S17_RS11280 (N4S17_11280) aroE 2318193..2319011 (-) 819 WP_000451211.1 shikimate dehydrogenase -
  N4S17_RS11285 (N4S17_11285) tsaC 2319016..2319588 (-) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  N4S17_RS11290 (N4S17_11290) yrdD 2319593..2320135 (-) 543 WP_001129723.1 type I DNA topoisomerase -
  N4S17_RS11295 (N4S17_11295) smg 2320164..2320637 (-) 474 WP_000460670.1 DUF494 family protein Smg -
  N4S17_RS11300 (N4S17_11300) dprA 2320609..2321733 (-) 1125 WP_000228519.1 DNA-protecting protein DprA Machinery gene
  N4S17_RS11305 (N4S17_11305) def 2321863..2322372 (+) 510 WP_000114984.1 peptide deformylase -
  N4S17_RS11310 (N4S17_11310) fmt 2322387..2323334 (+) 948 WP_000004432.1 methionyl-tRNA formyltransferase -
  N4S17_RS11315 (N4S17_11315) rsmB 2323380..2324669 (+) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  N4S17_RS11320 (N4S17_11320) trkA 2324691..2326067 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  N4S17_RS11325 (N4S17_11325) mscL 2326197..2326607 (+) 411 WP_000022439.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41006.95 Da        Isoelectric Point: 6.3839

>NTDB_id=629484 N4S17_RS11300 WP_000228519.1 2320609..2321733(-) (dprA) [Escherichia coli strain UC4224]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLQATTDYPGALFVEGELHALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=629484 N4S17_RS11300 WP_000228519.1 2320609..2321733(-) (dprA) [Escherichia coli strain UC4224]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACTGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCAGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCATAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACAAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTTTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTAGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Glaesserella parasuis strain SC1401

45.104

90.107

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393