Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   N1H43_RS20420 Genome accession   NZ_CP104442
Coordinates   4193604..4194728 (-) Length   374 a.a.
NCBI ID   WP_000228535.1    Uniprot ID   B7LHY7
Organism   Escherichia coli strain HBUT-L     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 4188604..4199728
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N1H43_RS20390 (N1H43_20395) yrdA 4190404..4190958 (+) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  N1H43_RS20395 (N1H43_20400) yrdB 4190934..4191191 (-) 258 WP_001070563.1 DUF1488 domain-containing protein -
  N1H43_RS20400 (N1H43_20405) aroE 4191188..4192006 (-) 819 WP_000451211.1 shikimate dehydrogenase -
  N1H43_RS20405 (N1H43_20410) tsaC 4192011..4192583 (-) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  N1H43_RS20410 (N1H43_20415) yrdD 4192588..4193130 (-) 543 WP_001129723.1 type I DNA topoisomerase -
  N1H43_RS20415 (N1H43_20420) smg 4193159..4193632 (-) 474 WP_000460670.1 DUF494 family protein Smg -
  N1H43_RS20420 (N1H43_20425) dprA 4193604..4194728 (-) 1125 WP_000228535.1 DNA-protecting protein DprA Machinery gene
  N1H43_RS20425 (N1H43_20430) def 4194858..4195367 (+) 510 WP_000114984.1 peptide deformylase -
  N1H43_RS20430 (N1H43_20435) fmt 4195382..4196329 (+) 948 WP_000004432.1 methionyl-tRNA formyltransferase -
  N1H43_RS20435 (N1H43_20440) rsmB 4196375..4197664 (+) 1290 WP_000744779.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  N1H43_RS20440 (N1H43_20445) trkA 4197686..4199062 (+) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  N1H43_RS20445 (N1H43_20450) mscL 4199192..4199602 (+) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40954.92 Da        Isoelectric Point: 6.3122

>NTDB_id=629430 N1H43_RS20420 WP_000228535.1 4193604..4194728(-) (dprA) [Escherichia coli strain HBUT-L]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLVTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=629430 N1H43_RS20420 WP_000228535.1 4193604..4194728(-) (dprA) [Escherichia coli strain HBUT-L]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGTGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT
TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LHY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

44.865

98.93

0.444
  dprA Vibrio cholerae strain A1552

44.865

98.93

0.444
  dprA Haemophilus influenzae Rd KW20

45.401

90.107

0.409
  dprA Glaesserella parasuis strain SC1401

44.838

90.642

0.406
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393