Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   N4R43_RS02020 Genome accession   NZ_CP104388
Coordinates   358364..359365 (-) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis strain LJL7m20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 353364..364365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4R43_RS01985 (N4R43_01985) - 353903..354391 (-) 489 WP_003131959.1 GAF domain-containing protein -
  N4R43_RS01990 (N4R43_01990) - 354626..354898 (+) 273 WP_003131960.1 chorismate mutase -
  N4R43_RS01995 (N4R43_01995) - 354987..355433 (-) 447 WP_003131961.1 hypothetical protein -
  N4R43_RS02000 (N4R43_02000) - 355651..355851 (+) 201 WP_058223263.1 hypothetical protein -
  N4R43_RS02005 (N4R43_02005) glnA 355910..357250 (-) 1341 WP_003131984.1 type I glutamate--ammonia ligase -
  N4R43_RS02010 (N4R43_02010) - 357312..357677 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  N4R43_RS02015 (N4R43_02015) - 357778..358215 (-) 438 WP_058211770.1 low molecular weight protein-tyrosine-phosphatase -
  N4R43_RS02020 (N4R43_02020) ruvB 358364..359365 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  N4R43_RS02025 (N4R43_02025) ruvA 359492..360085 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  N4R43_RS02030 (N4R43_02030) hexB 360195..362165 (-) 1971 WP_058223262.1 DNA mismatch repair endonuclease MutL Machinery gene
  N4R43_RS02035 (N4R43_02035) - 362295..362990 (-) 696 WP_010906384.1 hypothetical protein -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=629209 N4R43_RS02020 WP_003131994.1 358364..359365(-) (ruvB) [Lactococcus lactis strain LJL7m20]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=629209 N4R43_RS02020 WP_003131994.1 358364..359365(-) (ruvB) [Lactococcus lactis strain LJL7m20]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ATGTATTACTTTTTGGTCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGAGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTGGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAATATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGTTATGTATACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48