Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvA   Type   Machinery gene
Locus tag   N4R43_RS02025 Genome accession   NZ_CP104388
Coordinates   359492..360085 (-) Length   197 a.a.
NCBI ID   WP_003131995.1    Uniprot ID   Q9CDL2
Organism   Lactococcus lactis strain LJL7m20     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 354492..365085
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4R43_RS01990 (N4R43_01990) - 354626..354898 (+) 273 WP_003131960.1 chorismate mutase -
  N4R43_RS01995 (N4R43_01995) - 354987..355433 (-) 447 WP_003131961.1 hypothetical protein -
  N4R43_RS02000 (N4R43_02000) - 355651..355851 (+) 201 WP_058223263.1 hypothetical protein -
  N4R43_RS02005 (N4R43_02005) glnA 355910..357250 (-) 1341 WP_003131984.1 type I glutamate--ammonia ligase -
  N4R43_RS02010 (N4R43_02010) - 357312..357677 (-) 366 WP_003131985.1 MerR family transcriptional regulator -
  N4R43_RS02015 (N4R43_02015) - 357778..358215 (-) 438 WP_058211770.1 low molecular weight protein-tyrosine-phosphatase -
  N4R43_RS02020 (N4R43_02020) ruvB 358364..359365 (-) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  N4R43_RS02025 (N4R43_02025) ruvA 359492..360085 (-) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  N4R43_RS02030 (N4R43_02030) hexB 360195..362165 (-) 1971 WP_058223262.1 DNA mismatch repair endonuclease MutL Machinery gene
  N4R43_RS02035 (N4R43_02035) - 362295..362990 (-) 696 WP_010906384.1 hypothetical protein -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 21166.73 Da        Isoelectric Point: 5.8174

>NTDB_id=629210 N4R43_RS02025 WP_003131995.1 359492..360085(-) (ruvA) [Lactococcus lactis strain LJL7m20]
MFEYLNGKLVKISPTNIVIDVAGIGYLISVANPYAWSALMNTEVKIYVHQVIREDAHSLYGFVNEAEKALFLRLISVSGI
GPKSALAIIAAADNEGLITAIDNSDIKYLTKFPGVGKKTAMQMVLDLAGKFDATGTVGISLLDAGPAGNLALEEAIEALQ
ALGYKATELKKIEKKLAQETGLTSEEYIKSALKLMMK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=629210 N4R43_RS02025 WP_003131995.1 359492..360085(-) (ruvA) [Lactococcus lactis strain LJL7m20]
ATGTTTGAATATCTTAATGGAAAATTAGTAAAAATTTCCCCAACAAATATTGTAATTGATGTAGCAGGAATTGGTTATCT
TATCAGTGTAGCTAACCCTTACGCTTGGTCTGCTTTGATGAACACAGAAGTAAAAATTTATGTTCATCAAGTCATTCGCG
AAGATGCCCACAGCCTCTATGGTTTTGTTAACGAGGCCGAAAAAGCTTTATTCTTACGTCTGATCAGTGTTTCTGGGATT
GGGCCAAAATCAGCTCTGGCCATCATTGCGGCGGCTGATAACGAAGGTTTAATCACTGCTATTGACAATAGTGATATCAA
GTATTTAACTAAATTTCCAGGAGTTGGTAAAAAAACAGCCATGCAGATGGTGCTTGATTTGGCTGGGAAATTTGATGCGA
CAGGAACTGTAGGTATTTCTCTTCTTGATGCTGGACCTGCTGGCAATCTTGCTTTGGAAGAAGCGATTGAAGCGCTGCAA
GCTTTGGGTTATAAAGCAACAGAATTGAAGAAAATTGAGAAAAAATTAGCTCAAGAAACAGGTCTGACCAGCGAAGAATA
TATCAAATCAGCCTTAAAACTTATGATGAAATAA

Domains

Predicted by InterProScan.

RuvA N terminal domain (RuvA_N)

(1-61)

RuvA, C-terminal domain (RuvA_C)

(151-196)

Helix-hairpin-helix domain (HHH_5)

(71-127)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvA Streptococcus pneumoniae TIGR4

60.101

100

0.604
  ruvA Streptococcus pneumoniae R6

60.101

100

0.604
  ruvA Streptococcus pneumoniae D39

60.101

100

0.604
  ruvA Bacillus subtilis subsp. subtilis str. 168

44.828

100

0.462