Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   N4267_RS02305 Genome accession   NZ_CP104354
Coordinates   479863..480324 (-) Length   153 a.a.
NCBI ID   WP_162906052.1    Uniprot ID   -
Organism   Vibrio cholerae strain PNUSAV001140     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 474863..485324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4267_RS02285 (N4267_02285) coaE 475367..475975 (-) 609 WP_000011553.1 dephospho-CoA kinase -
  N4267_RS02290 (N4267_02290) pilD 475972..476847 (-) 876 WP_001960918.1 A24 family peptidase Machinery gene
  N4267_RS02295 (N4267_02295) pilC 476905..478131 (-) 1227 WP_000648511.1 type II secretion system F family protein Machinery gene
  N4267_RS02300 (N4267_02300) pilB 478174..479862 (-) 1689 WP_134987328.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N4267_RS02305 (N4267_02305) pilA 479863..480324 (-) 462 WP_162906052.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N4267_RS02310 (N4267_02310) nadC 480569..481459 (-) 891 WP_134987327.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N4267_RS02315 (N4267_02315) ampD 481755..482300 (+) 546 WP_001959072.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  N4267_RS02320 (N4267_02320) - 482344..483732 (-) 1389 WP_001959226.1 IS4-like element ISVa18 family transposase -
  N4267_RS02325 (N4267_02325) fldB 483871..484389 (-) 519 WP_000690112.1 flavodoxin FldB -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 15771.07 Da        Isoelectric Point: 8.1153

>NTDB_id=628873 N4267_RS02305 WP_162906052.1 479863..480324(-) (pilA) [Vibrio cholerae strain PNUSAV001140]
MKAYKNKQQQGFTLIELMIVVAIVGILAAFAVPAYQNYTMRAHASEMLSASSAMKTAVGICLISGNTNCSSGNGGVPAKQ
DLGAFSIESLAGASGASPTVQASVDSGQTKGKLVAGDTIKLTPTLNTAGVTWQVECVKAANQQSSIDDWCPVK

Nucleotide


Download         Length: 462 bp        

>NTDB_id=628873 N4267_RS02305 WP_162906052.1 479863..480324(-) (pilA) [Vibrio cholerae strain PNUSAV001140]
ATGAAAGCGTATAAAAACAAACAACAGCAAGGTTTTACCTTAATTGAATTGATGATTGTGGTGGCGATTGTGGGAATTTT
GGCTGCTTTTGCCGTTCCTGCATATCAAAATTACACAATGCGAGCTCATGCAAGCGAGATGCTTAGTGCATCTTCAGCCA
TGAAAACCGCTGTAGGAATATGTCTAATTAGTGGTAATACAAACTGTTCATCGGGAAATGGTGGAGTTCCAGCTAAACAA
GATCTTGGTGCGTTTTCTATAGAATCTCTTGCAGGGGCTAGCGGCGCGTCACCAACAGTACAAGCAAGTGTTGATTCTGG
GCAAACTAAAGGAAAACTAGTAGCAGGGGACACAATAAAACTAACACCAACCTTGAACACGGCTGGTGTAACATGGCAGG
TTGAATGTGTGAAGGCGGCAAATCAACAAAGTTCCATTGATGATTGGTGTCCTGTCAAATAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-31)

Pilin (bacterial filament) (Pilin)

(41-133)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio campbellii strain DS40M4

41.358

100

0.438
  comP Acinetobacter baylyi ADP1

44.697

86.275

0.386
  pilA Haemophilus influenzae 86-028NP

42.748

85.621

0.366
  pilA2 Legionella pneumophila strain ERS1305867

42.424

86.275

0.366