Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilV   Type   Machinery gene
Locus tag   AA2Tth_RS06400 Genome accession   NZ_AP019792
Coordinates   1195156..1195506 (+) Length   116 a.a.
NCBI ID   WP_143585831.1    Uniprot ID   -
Organism   Thermus thermophilus strain AA2-20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1190156..1200506
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA2Tth_RS06375 (TthAA220_12480) pilA/pilA1 1190973..1191443 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  AA2Tth_RS06380 (TthAA220_12490) pilA/pilA1 1191499..1191972 (+) 474 WP_143585823.1 GspH/FimT family protein Machinery gene
  AA2Tth_RS06385 (TthAA220_12500) pilA/pilA2 1191990..1192550 (+) 561 WP_242004637.1 type II secretion system protein Machinery gene
  AA2Tth_RS06390 (TthAA220_12510) pilA/pilA3 1192547..1193248 (+) 702 WP_143585827.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AA2Tth_RS06395 (TthAA220_12520) comZ 1193259..1194935 (+) 1677 WP_143585829.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  AA2Tth_RS06400 (TthAA220_12530) pilV 1195156..1195506 (+) 351 WP_143585831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AA2Tth_RS06410 (TthAA220_12540) - 1195755..1196135 (+) 381 WP_018461702.1 DUF1640 domain-containing protein -
  AA2Tth_RS06415 (TthAA220_12550) - 1196211..1196753 (+) 543 WP_143585835.1 Uma2 family endonuclease -
  AA2Tth_RS06420 (TthAA220_12560) - 1196775..1197539 (-) 765 WP_242004638.1 IS982 family transposase -
  AA2Tth_RS06425 (TthAA220_12570) - 1197626..1198726 (+) 1101 WP_143584933.1 transposase -
  AA2Tth_RS06430 (TthAA220_12580) - 1198786..1200006 (+) 1221 WP_143585839.1 IS256-like element ISTth4 family transposase -
  AA2Tth_RS06435 (TthAA220_12590) - 1200155..1200316 (+) 162 WP_143585841.1 hypothetical protein -

Sequence

Protein


Download         Length: 116 a.a.        Molecular weight: 12102.76 Da        Isoelectric Point: 4.0390

>NTDB_id=62791 AA2Tth_RS06400 WP_143585831.1 1195156..1195506(+) (pilV) [Thermus thermophilus strain AA2-20]
MRNAKGFTLIELLIVIAIIAILAAVLIPNVLNARKRATDSAAQAFGRQVITWLAAADTAGVDVTEVTSCTDELLQAEGAP
AELPNGVAGCSIEYDGAGQWTVTVTSDAGNTFTFTY

Nucleotide


Download         Length: 351 bp        

>NTDB_id=62791 AA2Tth_RS06400 WP_143585831.1 1195156..1195506(+) (pilV) [Thermus thermophilus strain AA2-20]
ATGCGGAACGCGAAAGGCTTCACCCTGATTGAGCTCCTGATCGTCATCGCCATCATCGCCATCCTGGCGGCGGTGCTGAT
CCCGAACGTCTTGAACGCCCGCAAGCGGGCCACCGATAGCGCCGCTCAGGCCTTTGGCCGCCAAGTCATCACCTGGCTCG
CCGCCGCTGACACGGCCGGTGTGGACGTCACTGAGGTCACGAGCTGTACCGACGAGCTCCTTCAAGCGGAAGGGGCTCCC
GCTGAACTCCCCAATGGGGTAGCTGGGTGCTCCATCGAGTACGATGGCGCCGGTCAGTGGACGGTGACGGTCACGTCTGA
TGCCGGTAACACGTTCACCTTCACTTACTGA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin A4 (PilA4)

(39-114)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.966

100

0.44
  pilA2 Legionella pneumophila str. Paris

41.739

99.138

0.414
  pilA2 Legionella pneumophila strain ERS1305867

40.87

99.138

0.405
  pilA/pilAI Pseudomonas stutzeri DSM 10701

32.812

100

0.362

Multiple sequence alignment