Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY28_RS01920 Genome accession   NZ_CP087194
Coordinates   424796..425860 (+) Length   354 a.a.
NCBI ID   WP_018928618.1    Uniprot ID   A0A2W7JGQ6
Organism   Pseudomonas sp. B21-017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 419796..430860
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY28_RS01910 (LOY28_01910) - 420738..422006 (+) 1269 WP_018929144.1 malic enzyme-like NAD(P)-binding protein -
  LOY28_RS01915 (LOY28_01915) - 422140..424581 (-) 2442 WP_018928619.1 penicillin-binding protein 1A -
  LOY28_RS01920 (LOY28_01920) comM 424796..425860 (+) 1065 WP_018928618.1 pilus assembly protein PilM Machinery gene
  LOY28_RS01925 (LOY28_01925) - 425860..426426 (+) 567 WP_018928617.1 PilN domain-containing protein -
  LOY28_RS01930 (LOY28_01930) pilO 426423..427046 (+) 624 WP_018928616.1 type 4a pilus biogenesis protein PilO -
  LOY28_RS01935 (LOY28_01935) - 427043..427570 (+) 528 WP_018928615.1 pilus assembly protein PilP -
  LOY28_RS01940 (LOY28_01940) pilQ 427584..429659 (+) 2076 WP_018928614.1 type IV pilus secretin PilQ Machinery gene
  LOY28_RS01945 (LOY28_01945) aroK 429664..430182 (+) 519 WP_019581860.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38080.46 Da        Isoelectric Point: 4.6675

>NTDB_id=627808 LOY28_RS01920 WP_018928618.1 424796..425860(+) (comM) [Pseudomonas sp. B21-017]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPASAVVEKNIAELEGVGQALARVLLKAKTSQRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGASVRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQEHLTVAVVDIGATMTTLSVLNNGRIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFRDALVQQVSRSLQFFFASGQYSSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMTLSSRVNASALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627808 LOY28_RS01920 WP_018928618.1 424796..425860(+) (comM) [Pseudomonas sp. B21-017]
GTGCTAGGACTCTTCAATAAAAAAACCAATGCGCTTCTGGGGATCGACATCAGCTCTACGTCGGTGAAGCTGTTGGAGTT
AAGCCGCCAAGGCGACCGTTATCGGGTCGAGGCCTATGCGGTCGAGCCGCTGCCCGCCAGTGCCGTGGTCGAAAAAAATA
TTGCCGAGCTCGAAGGCGTGGGGCAGGCCCTTGCGCGCGTGCTGCTCAAGGCCAAAACCAGCCAGAGGAACGTCGCCGTA
GCGGTCGCCGGCTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAGATGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCGTATCCGCTGGATGAAGTGGCGATCGACTTTGAAGTGCAAGGCGCGTCAGTGC
GCAACCCCGAGCGGGTCAATGTGCTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAGGTTCGTGAAGCGGCCCTGGCCCTG
GCCGGGCTGACCGCGCGAGTAGTCGATGTCGAAGCCTATGCCCTGGAGCGAGCGTTCGGCTTGCTGGCAACCCAGCTGGC
CGCCTCTCAGGAGCATCTGACCGTGGCGGTAGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGTTGAATAATGGGC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGCCGACAACTGACGGAAGAAATCCAGCGCCGTTATGGCCTGACGGTC
GAGCAGGCTGGGTTGGCGAAGAAACAGGGTGGTTTGCCGGACGATTACGTCAGTGAGGTCTTGCAGCCGTTTCGGGATGC
GTTGGTGCAACAAGTATCGCGCTCATTGCAGTTTTTCTTTGCCTCCGGCCAATACAGCTCGGTCGATCACATTCTCCTGG
CCGGCGGCACTGCCTCGGTCCCAGGGCTGGATCGGTTGATCGAGCAGCGTCTGGGTACGCCGACCCAGGTCGCTAACCCA
TTCTCCGACATGACCCTGAGCAGCAGGGTCAACGCCAGTGCCCTGGCGAGCGATGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2W7JGQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

57.062

100

0.571
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483