Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY28_RS01940 Genome accession   NZ_CP087194
Coordinates   427584..429659 (+) Length   691 a.a.
NCBI ID   WP_018928614.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-017     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 422584..434659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY28_RS01920 (LOY28_01920) comM 424796..425860 (+) 1065 WP_018928618.1 pilus assembly protein PilM Machinery gene
  LOY28_RS01925 (LOY28_01925) - 425860..426426 (+) 567 WP_018928617.1 PilN domain-containing protein -
  LOY28_RS01930 (LOY28_01930) pilO 426423..427046 (+) 624 WP_018928616.1 type 4a pilus biogenesis protein PilO -
  LOY28_RS01935 (LOY28_01935) - 427043..427570 (+) 528 WP_018928615.1 pilus assembly protein PilP -
  LOY28_RS01940 (LOY28_01940) pilQ 427584..429659 (+) 2076 WP_018928614.1 type IV pilus secretin PilQ Machinery gene
  LOY28_RS01945 (LOY28_01945) aroK 429664..430182 (+) 519 WP_019581860.1 shikimate kinase AroK -
  LOY28_RS01950 (LOY28_01950) aroB 430333..431433 (+) 1101 WP_258612987.1 3-dehydroquinate synthase -
  LOY28_RS01955 (LOY28_01955) - 431444..433051 (+) 1608 WP_018928612.1 AAA family ATPase -

Sequence

Protein


Download         Length: 691 a.a.        Molecular weight: 74798.98 Da        Isoelectric Point: 5.7805

>NTDB_id=627809 LOY28_RS01940 WP_018928614.1 427584..429659(+) (pilQ) [Pseudomonas sp. B21-017]
MNRIFSTFGISLWIAFLSPLVQAANLKALDVAALPGDRVELKLSFDGPPPQPHGYTTEQPARIALDLPGVTSQLANKSRD
LGGGNARSATVVEAKDRTRLIIGLTQLTPYNARVEGNNLFVVVGQAPGNKASKPIASAPRAATAVPAPARASAPVGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIIVGFTKTQLPERLRVRLDVKDFATPVQFVNASATGDRAIISIEPSG
AFDYSTYQTDNKLTISIRPMTVDDLQKRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KVDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQKGNWNGSGVNG
SSTTVGTPGSTSTNSPFVDLGTSSNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPYQ
EASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSNT
QSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2076 bp        

>NTDB_id=627809 LOY28_RS01940 WP_018928614.1 427584..429659(+) (pilQ) [Pseudomonas sp. B21-017]
ATGAACAGGATTTTCTCAACCTTCGGTATTTCGCTATGGATAGCGTTTCTGTCGCCGTTGGTACAGGCGGCCAACCTCAA
AGCGCTGGATGTCGCCGCGCTGCCTGGTGACCGTGTCGAGCTGAAGTTGTCGTTCGACGGGCCGCCACCGCAGCCTCATG
GCTATACGACTGAGCAGCCTGCGCGGATTGCGCTGGATTTGCCGGGAGTTACCAGTCAGCTGGCGAACAAGAGTCGTGAC
CTGGGCGGCGGCAATGCCCGCAGCGCCACGGTGGTGGAAGCCAAGGACCGCACGCGGCTGATCATCGGTCTGACTCAACT
GACTCCGTATAACGCACGCGTCGAAGGCAACAACCTGTTCGTGGTCGTCGGGCAGGCGCCCGGTAACAAGGCGTCCAAAC
CGATTGCCAGCGCACCACGCGCGGCCACGGCGGTACCCGCACCCGCCAGAGCCAGTGCTCCCGTGGGCAAGGCTATTCGT
GGCGTGGATTTCCAGCGTGGCACCCAGGGTGAAGGCAACGTGGTTATCGATCTGTCGGATCCGTCCATCGCCCCGGACAT
TCAGGAACGCGATGGCAAGATCATCGTCGGCTTCACCAAAACCCAATTGCCTGAGCGGTTGCGCGTCCGTCTCGACGTCA
AGGATTTCGCTACCCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGACCGGGCCATCATCAGCATCGAGCCCAGCGGC
GCCTTCGATTATTCGACCTACCAGACCGATAACAAACTGACCATCAGCATCCGGCCGATGACTGTCGATGACTTGCAAAA
ACGCAACGCCGAACGTTTTGCCTACAGCGGCGAAAAGCTCTCGTTGAATTTCCAGGACATCGATGTGCGCTCGGTGCTGC
AATTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGTGATACGGTGCAGGGCGGCATCACGTTGCGCCTGCAGAAT
GTACCGTGGGATCAGGCGCTGGACCTGGTGTTGAAAACCAAAGGCCTGGATAAGCGCAAAATTGGCAATGTGTTGCTGGT
GGCGCCGGCCGATGAAATCGCCGCCCGGGAGCGTCAGGAGCTGGAGTCGCAGAAACAAATCGCCGAACTGGCGCCTCTGC
GTCGCGAGCTGTTGCAGGTCAACTACGCCAAGGCTGCCGATATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAGGCG
AAAGTCGACGAGCGCGGTTCAATCACTGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCGCCTCGA
CGAGCTGCGTCGCATCGTTGCGCAGCTGGATATTCCAGTGCGTCAGGTGATGATCGAGGCGCGCATCGTTGAAGCCAACG
TCGATTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCGGTGCAGAAGGGTAATTGGAACGGCTCCGGGGTCAACGGC
TCTTCGACCACCGTCGGCACGCCGGGCAGCACCAGCACCAACTCGCCGTTCGTCGATCTTGGGACTTCCTCCAACACCTC
GGGGATCGGCATCGCCTTCATTACCGACAATGTGCTGCTGGACCTTGAGCTGACGGCCATGGAAAAAACCGGCAACGGGG
AAATCGTCTCGCAGCCCAAGGTGGTCACGTCAGACAAGGAAACCGCGAAAATCCTCAAGGGCACCGAAATCCCCTATCAG
GAAGCCAGCTCCAGCGGTGCCACTTCAGTGTCGTTCAAGGAGGCTTCGCTGTCCCTGGAAGTGACACCGCAGATCACCCC
GGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAAGTGCAGGATGTACCGCCGA
TCAAGAAAAACGAGGTCAACGCCAAGGTATTGGTCAACGACGGCGAGACCATCGTGATTGGTGGGGTTTTCTCAAATACT
CAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGTGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGTGGTTTC
GGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAATCAGGCGATTGCTGTGAGTCATTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(526-682)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

AMIN domain (AMIN)

(37-121)

Secretin and TonB N terminus short domain (STN)

(303-349)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.829

100

0.753
  comQ Acinetobacter baylyi ADP1

41.541

100

0.437
  pilQ Acinetobacter baumannii D1279779

39.309

100

0.428
  pilQ Acinetobacter baumannii strain A118

39.309

100

0.428
  pilQ Legionella pneumophila strain Lp02

37.611

98.119

0.369
  pilQ Legionella pneumophila strain ERS1305867

37.518

97.974

0.368