Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   LOY31_RS02070 Genome accession   NZ_CP087192
Coordinates   464306..465370 (+) Length   354 a.a.
NCBI ID   WP_007952389.1    Uniprot ID   J2PGW6
Organism   Pseudomonas sp. B21-021     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459306..470370
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY31_RS02060 (LOY31_02060) - 460103..461371 (+) 1269 WP_007952395.1 malic enzyme-like NAD(P)-binding protein -
  LOY31_RS02065 (LOY31_02065) - 461642..464085 (-) 2444 Protein_403 penicillin-binding protein 1A -
  LOY31_RS02070 (LOY31_02070) comM 464306..465370 (+) 1065 WP_007952389.1 pilus assembly protein PilM Machinery gene
  LOY31_RS02075 (LOY31_02075) - 465370..465936 (+) 567 WP_007952387.1 PilN domain-containing protein -
  LOY31_RS02080 (LOY31_02080) pilO 465933..466556 (+) 624 WP_085608413.1 type 4a pilus biogenesis protein PilO -
  LOY31_RS02085 (LOY31_02085) - 466553..467077 (+) 525 WP_258712762.1 pilus assembly protein PilP -
  LOY31_RS02090 (LOY31_02090) pilQ 467092..469170 (+) 2079 WP_258712763.1 type IV pilus secretin PilQ Machinery gene
  LOY31_RS02095 (LOY31_02095) aroK 469175..469693 (+) 519 WP_045121685.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38097.60 Da        Isoelectric Point: 4.6857

>NTDB_id=627749 LOY31_RS02070 WP_007952389.1 464306..465370(+) (comM) [Pseudomonas sp. B21-021]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPASAVVEKNIAELEGVGQALSRVLVKARTGLKTVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEERLNTPTQVANP
FSDMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627749 LOY31_RS02070 WP_007952389.1 464306..465370(+) (comM) [Pseudomonas sp. B21-021]
GTGCTAGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGCTCCACATCGGTGAAGCTGCTGGAGCT
GAGCCGTCAGGGTGAGCGCTACCGGGTCGAGGCGTACGCGGTGGAGCCGTTGCCGGCGAGTGCCGTGGTCGAAAAGAACA
TCGCCGAGCTCGAGGGCGTCGGCCAGGCCCTGTCCCGGGTGCTGGTCAAGGCGCGCACCGGGCTGAAAACCGTTGCGGTG
GCAGTGGCGGGTTCGGCGGTGATCACCAAGGTCATCGAGATGGACGCCGGGCTGTCCGACGATGAACTTGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCCGAGCGGGTCAACGTACTGCTGGCCGCCTGTCGCAAGGAAAACGTCGAAGTCCGCGAAGCGGCGCTCGCCCTC
GCCGGGCTGACCGCGCGGGTGGTGGATGTCGAAGCCTACGCACTGGAACGCTCGTTCGGCCTGCTGGCGACGCAACTGGC
CGCGTCCCAGGACCGGCTGACAGTGGCCGTGGTCGACATCGGCGCCACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTATACCCGCGAACAGCTGTTCGGCGGTCGACAGCTGACCGAGGAAATCCAGCGCCGTTATGGCCTGACCCTT
GAACAGGCGGGACTCGCCAAGAAGCAGGGCGGCCTGCCGGACGACTACGTCAGCGAGGTGCTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAGGTGTCGCGCTCCCTGCAGTTCTTTTTCGCCTCCGGCCAGTACAACGCCGTCGACCACATTCTGCTGG
CCGGCGGCACGGCGTCGGTGCCCGGGCTCGACCGGCTGATCGAAGAGCGCCTGAACACACCGACCCAGGTGGCCAACCCG
TTTTCCGACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCCCTGGCGAGCGATGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2PGW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

49.577

100

0.497