Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY41_RS01905 Genome accession   NZ_CP087187
Coordinates   433691..434755 (+) Length   354 a.a.
NCBI ID   WP_024011266.1    Uniprot ID   V8RDF4
Organism   Pseudomonas atacamensis strain B21-026     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428691..439755
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY41_RS01895 (LOY41_01890) - 429438..430706 (+) 1269 WP_016772053.1 malic enzyme-like NAD(P)-binding protein -
  LOY41_RS01900 (LOY41_01895) - 431027..433471 (-) 2445 WP_373877946.1 penicillin-binding protein 1A -
  LOY41_RS01905 (LOY41_01900) pilM 433691..434755 (+) 1065 WP_024011266.1 pilus assembly protein PilM Machinery gene
  LOY41_RS01910 (LOY41_01905) - 434755..435321 (+) 567 WP_016772051.1 PilN domain-containing protein -
  LOY41_RS01915 (LOY41_01910) pilO 435318..435941 (+) 624 WP_122705136.1 type 4a pilus biogenesis protein PilO -
  LOY41_RS01920 (LOY41_01915) - 435938..436462 (+) 525 WP_073471601.1 pilus assembly protein PilP -
  LOY41_RS01925 (LOY41_01920) pilQ 436476..438554 (+) 2079 WP_122705134.1 type IV pilus secretin PilQ Machinery gene
  LOY41_RS01930 (LOY41_01925) aroK 438559..439077 (+) 519 WP_073471603.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38045.57 Da        Isoelectric Point: 4.7539

>NTDB_id=627623 LOY41_RS01905 WP_024011266.1 433691..434755(+) (pilM) [Pseudomonas atacamensis strain B21-026]
MLGLFNKKTNALLGIDISSTSVKLLELSRQGERYRVEAYAVEPLPANAVVEKNIAELEGVGQALSRLLVKARTGVKSVAV
AVAGSAVITKVIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATHLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627623 LOY41_RS01905 WP_024011266.1 433691..434755(+) (pilM) [Pseudomonas atacamensis strain B21-026]
GTGCTGGGACTCTTCAATAAAAAGACCAATGCGTTACTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTGGAACT
GAGCCGTCAGGGCGAGCGCTACCGGGTCGAGGCCTACGCGGTGGAGCCGTTGCCGGCGAACGCTGTGGTCGAAAAGAACA
TCGCCGAACTCGAGGGCGTTGGCCAGGCACTGAGCCGCCTGCTGGTCAAGGCGCGTACCGGGGTCAAGAGCGTGGCAGTG
GCGGTGGCCGGTTCGGCGGTGATCACCAAGGTTATCGAGATGGACGCCGGGCTGTCCGACGACGAACTGGAAAACCAGCT
CAAGATCGAGGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGACTTTGAAGTCCAGGGCGTCTCGCCGC
GCAACCCGGAACGGGTCAACGTGCTGCTTGCCGCCTGTCGCAAGGAAAACGTCGAGGTGCGTGAAGCGGCGCTGGCGCTC
GCCGGCCTGACCGCGCGGGTGGTGGATGTCGAGGCCTACGCGCTGGAGCGCTCGTTCGGCCTGCTGGCGACGCATCTGGC
GGCCTCCCAGGAGCGCCTGACCGTGGCAGTGGTCGACATCGGTGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAATTGTTCGGCGGACGCCAGCTCACTGAAGAGATCCAGCGCCGCTACGGCCTCACGGTC
GAACAGGCGGGCCTGGCGAAAAAACAGGGCGGGCTGCCTGACGATTACGTCAGCGAGGTCCTGCAGCCATTTCGCGAGGC
GCTGGTGCAGCAAGTCTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGCCAGTACAACGCGGTCGACCATATCCTGCTGG
CCGGCGGCACCGCCTCGGTGCCCGGCCTGGACCGTCTGATCGAACAGCGTCTGGACACGCCGACGCAGGTCGCCAACCCG
TTCGCCGACATGGCTTTAGGCAGCAAGGTCAATGCTGGGGCGCTGGCCAGTGATGCGCCAGCCTTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V8RDF4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483