Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY55_RS01920 Genome accession   NZ_CP087176
Coordinates   417253..418317 (+) Length   354 a.a.
NCBI ID   WP_223525454.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-040     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 412253..423317
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY55_RS01910 (LOY55_01910) - 413082..414350 (+) 1269 WP_046031175.1 malic enzyme-like NAD(P)-binding protein -
  LOY55_RS01915 (LOY55_01915) - 414595..417036 (-) 2442 WP_371916746.1 penicillin-binding protein 1A -
  LOY55_RS01920 (LOY55_01920) pilM 417253..418317 (+) 1065 WP_223525454.1 pilus assembly protein PilM Machinery gene
  LOY55_RS01925 (LOY55_01925) - 418317..418883 (+) 567 WP_046031172.1 PilN domain-containing protein -
  LOY55_RS01930 (LOY55_01930) pilO 418880..419503 (+) 624 WP_109785373.1 type 4a pilus biogenesis protein PilO -
  LOY55_RS01935 (LOY55_01935) - 419500..420027 (+) 528 WP_223525453.1 pilus assembly protein PilP -
  LOY55_RS01940 (LOY55_01940) pilQ 420041..422104 (+) 2064 WP_046031169.1 type IV pilus secretin PilQ family protein Machinery gene
  LOY55_RS01945 (LOY55_01945) aroK 422109..422627 (+) 519 WP_046031168.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38165.76 Da        Isoelectric Point: 4.7417

>NTDB_id=627346 LOY55_RS01920 WP_223525454.1 417253..418317(+) (pilM) [Pseudomonas sp. B21-040]
MLGLFNKKANSLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGLALSRVLLKARTSLKSVAV
AVAGSAVITKTIEMDGGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVSVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFSLLSTQLPAPREHLTVAVVDIGATMTTLSVLYDGKIIYTREQLFGGRQLTEEIQRRYGLTV
EQAGLAKKQGGLPDDYVSEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLGTSTQVANP
FLNMTLSSKVNAGALLSDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627346 LOY55_RS01920 WP_223525454.1 417253..418317(+) (pilM) [Pseudomonas sp. B21-040]
GTGCTAGGACTCTTCAATAAAAAAGCCAACTCGTTGTTGGGGATCGACATCAGCTCGACATCGGTAAAGCTTCTGGAACT
CAGCCGCCAGGGCGATCGCTATCGGGTCGAGGCCTACGCGGTAGAGCCGTTGCCTGCCAATGCCGTGGTCGAGAAAAATA
TCGCCGAACTCGAAGGCGTCGGGTTGGCGCTCTCGCGAGTGCTGCTCAAGGCCCGGACCAGCCTCAAGAGCGTGGCGGTG
GCCGTGGCCGGTTCGGCGGTGATCACTAAGACCATCGAGATGGACGGCGGACTTTCCGACGACGAGTTGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATCCCCTATCCGCTGGATGAAGTCGCCATCGACTTCGAAGTCCAGGGCGTCTCTGCGC
GTAACCCCGAGCGCGTCAGTGTATTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGTGAAGCGGCGCTGGCCCTG
GCCGGCCTGACTGCGCGCGTAGTCGATGTCGAGGCCTACGCACTGGAGCGTTCTTTCAGTTTGCTGTCGACGCAACTGCC
CGCCCCTCGGGAGCACCTGACGGTCGCGGTGGTGGACATCGGCGCGACCATGACCACCCTCAGCGTGTTGTACGACGGGA
AGATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGGCAACTGACTGAGGAAATTCAGCGACGTTATGGTCTGACGGTC
GAGCAGGCAGGGCTGGCGAAGAAGCAGGGCGGTTTGCCGGACGACTACGTCAGCGAAGTCTTGCAACCGTTTCGCGAGGC
GCTGGTGCAACAGGTTTCACGCTCGTTGCAGTTCTTTTTTGCCTCCGGTCAGTACAACGCGGTCGACCACATTCTACTGG
CCGGTGGCACGGCGTCGGTGCCGGGGCTTGACCGGTTGATCGAGCAGCGCCTGGGCACTTCCACTCAGGTGGCCAACCCG
TTTTTGAACATGACCTTGAGCAGCAAAGTTAACGCCGGTGCCCTGCTCAGTGATGCGCCAGCCCTGATGATTGCGTGCGG
GCTCGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.65

100

0.557
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475