Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LOY43_RS02000 Genome accession   NZ_CP087175
Coordinates   457480..458544 (+) Length   354 a.a.
NCBI ID   WP_093430076.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 452480..463544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY43_RS01990 (LOY43_01985) - 453228..454496 (+) 1269 WP_090280412.1 malic enzyme-like NAD(P)-binding protein -
  LOY43_RS01995 (LOY43_01990) - 454816..457260 (-) 2445 WP_407082808.1 penicillin-binding protein 1A -
  LOY43_RS02000 (LOY43_01995) pilM 457480..458544 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  LOY43_RS02005 (LOY43_02000) - 458544..459110 (+) 567 WP_095047205.1 PilN domain-containing protein -
  LOY43_RS02010 (LOY43_02005) pilO 459107..459730 (+) 624 WP_095047206.1 type 4a pilus biogenesis protein PilO -
  LOY43_RS02015 (LOY43_02010) - 459727..460254 (+) 528 WP_258709124.1 pilus assembly protein PilP -
  LOY43_RS02020 (LOY43_02015) pilQ 460268..462346 (+) 2079 WP_095047208.1 type IV pilus secretin PilQ family protein Machinery gene
  LOY43_RS02025 (LOY43_02020) aroK 462351..462869 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38107.72 Da        Isoelectric Point: 4.8109

>NTDB_id=627319 LOY43_RS02000 WP_093430076.1 457480..458544(+) (pilM) [Pseudomonas sp. B21-041]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRLLVKARTGLKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLSTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=627319 LOY43_RS02000 WP_093430076.1 457480..458544(+) (pilM) [Pseudomonas sp. B21-041]
GTGCTGGGACTCTTCAATAAAAAGGCCAATACGTTACTGGGGATCGACATCAGTTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTAGAGCCATTGCCAGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTTGAAGGCGTGGGTCAGGCCCTGAGCCGTTTACTGGTCAAGGCGCGCACCGGGCTCAAGAGCGTGGCAGTC
GCAGTGGCCGGTTCTGCCGTGATCACCAAGATCATCGAGATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAACCAGCT
GAAGATCGAGGCCGATCAGTACATTCCCTACCCGCTGGACGAAGTCGCCATCGATTTCGAGGTCCAGGGCGTGTCACCGC
GCAACCCTGAGCGGGTCAATGTGTTGCTCGCTGCCTGTCGCAAGGAAAACGTCGAAGTTCGTGAAGCGGCGCTGGCCCTC
GCCGGGCTGACCGCACGGGTAGTCGACGTCGAAGCCTATGCACTGGAGCGCTCGTTCGGCTTGCTGGCCACGCAACTGGC
GGCTTCTCAGGAGCGTCTGACTGTCGCTGTGGTCGACATCGGCGCGACCATGACCACGCTCAGCGTCCTGCACAATGGCA
AGATCATCTATACCCGCGAGCAATTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGCCGCTATGGCCTGACCCTC
GAACAAGCCGGGCTGGCGAAAAAACAGGGCGGTCTGCCCGATGATTACATCAGCGAGGTCTTGCAACCGTTTCGCGAGGC
GCTGGTGCAGCAAGTGTCGCGCTCGCTGCAGTTCTTCTTCGCCTCCGGGCAATACAACGCGGTCGATCACATTCTGCTGG
CCGGCGGCACGGCTTCAGTGCCCGGACTGGATCGGCTGATCGAGCAGCGCCTGAGCACGCCGACTCAAGTAGCCAACCCG
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCTTTGGCCAGCGACGCGCCGGCGCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475