Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LOY43_RS02020 Genome accession   NZ_CP087175
Coordinates   460268..462346 (+) Length   692 a.a.
NCBI ID   WP_095047208.1    Uniprot ID   -
Organism   Pseudomonas sp. B21-041     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 455268..467346
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LOY43_RS02000 (LOY43_01995) pilM 457480..458544 (+) 1065 WP_093430076.1 pilus assembly protein PilM Machinery gene
  LOY43_RS02005 (LOY43_02000) - 458544..459110 (+) 567 WP_095047205.1 PilN domain-containing protein -
  LOY43_RS02010 (LOY43_02005) pilO 459107..459730 (+) 624 WP_095047206.1 type 4a pilus biogenesis protein PilO -
  LOY43_RS02015 (LOY43_02010) - 459727..460254 (+) 528 WP_258709124.1 pilus assembly protein PilP -
  LOY43_RS02020 (LOY43_02015) pilQ 460268..462346 (+) 2079 WP_095047208.1 type IV pilus secretin PilQ family protein Machinery gene
  LOY43_RS02025 (LOY43_02020) aroK 462351..462869 (+) 519 WP_011332081.1 shikimate kinase AroK -
  LOY43_RS02030 (LOY43_02025) aroB 463022..464122 (+) 1101 WP_093430081.1 3-dehydroquinate synthase -
  LOY43_RS02035 (LOY43_02030) - 464133..465722 (+) 1590 WP_095047209.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74996.52 Da        Isoelectric Point: 5.8300

>NTDB_id=627320 LOY43_RS02020 WP_095047208.1 460268..462346(+) (pilQ) [Pseudomonas sp. B21-041]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKERTRLIVSLTQLAPYTTRVEGNNLFVVVGQGAPAAASRPAAVAPRTTAKAAAPAKAFVPKSRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSIRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNASGVN
GSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFSN
TQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=627320 LOY43_RS02020 WP_095047208.1 460268..462346(+) (pilQ) [Pseudomonas sp. B21-041]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCACCGATGGTACTCGCGGCCAATCTGAA
GACGCTCGACGTGGCGGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCACAACCCAAGG
GCTACACCACCGAATCGCCGGCGCGGATTGCCCTGGACCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAACCTCGAT
CTGGGCAGTGGCAATGCGCGCACCGCCACTGTGGTTGAGGCCAAGGAGCGTACGCGGCTGATTGTCAGCCTGACGCAATT
GGCGCCTTACACCACGCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCAGCAGCTGCGTCGCGCC
CTGCAGCCGTTGCGCCCCGCACTACGGCTAAAGCGGCCGCACCCGCCAAGGCATTTGTGCCGAAAAGCCGGGCGATTCGC
GGTGTGGACTTCCAGCGTGGTACGGCGGGGGAAGGCAATGTGGTCATCGACCTGTCCGATCCGACCATCGCCCCGGATAT
CCAGGAACATGACGGCAAGATCATCCTCAGCTTCGCGCGCACGCAGTTGCCGGACAAGTTGCGGGTACGCCTCGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTAACCGCTGATCGGACAGTTATTACGGTTGAACCCAGTGGC
ACCTACGAGTACTCCACCTTCCAGACTGACAACAAACTGACCGTCAGCATCCGCCCGATGACCGTCGATGACCTGCAAAA
ACGCAACGCTGACCGTCAGGCGTACGTCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATTTGGTTGCCAGCGACACGGTGCAGGGTGGCATCACCTTGCGTCTGCAAAAT
GTGCCGTGGGATCAGGCCCTGGATCTGGTGTTGAAAACCAAAGGCCTGGATAAGCGCAAGATCGGCAATGTGCTGCTGGT
GGCGCCGGCCGATGAAATCGCTGCCCGCGAGCGTCAGGAACTGGAGTCGCAGAAGCAGATCGCCGAACTGGCGCCGTTGC
GCCGCGAGCTGCTACAGGTGAACTACGCTAAAGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCG
AAAATCGATGAACGCGGTTCGATTACCGTTGATGAGCGCACCAACAACATCATTGCCTACCAGACTCAGGATCGCCTCGA
CGAACTGCGGCGGATCGTGGCGCAACTGGATATTCCGGTGCGTCAGGTGATGATCGAAGCACGGATTGTCGAGGCCAACG
TCGATTACGACAAAAGTCTGGGCGTGCGCTGGGGCGGCTCGATCCAGAACAAGGGCAACTGGAACGCTTCCGGGGTCAAC
GGTTCATCGACCACCATTGGTACGCCGGGCAGTACCAGCACCAATTCGCCGTTCGTCGACATGGGCACTGTGGGTAATAC
ATCGGGGATCGGCATTGCCTTCATTACCGACAACGTCCTGCTTGATCTTGAGCTGACTGCCATGGAGAAGACCGGCAACG
GCGAAATCGTCTCGCAGCCGAAGGTTGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCGTAT
CAGGAAGCCAGCTCCAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGCCGC
CGATCAAGAAAAACGAGGTCAACGCCAAGGTACTAGTCAATGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCAAAT
ACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGTGGT
ATCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(527-683)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.324

100

0.759
  comQ Acinetobacter baylyi ADP1

41.643

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.462

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.462

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.87

97.688

0.37
  pilQ Legionella pneumophila strain Lp02

37.87

97.688

0.37