Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LK336_RS02250 Genome accession   NZ_CP086572
Coordinates   432704..433678 (+) Length   324 a.a.
NCBI ID   WP_031787262.1    Uniprot ID   A0A7U7JUV7
Organism   Staphylococcus argenteus strain RIVM_M036020     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 427704..438678
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LK336_RS02215 (LK336_02215) rpmG 428161..428310 (+) 150 WP_001265709.1 50S ribosomal protein L33 -
  LK336_RS02220 (LK336_02220) - 428522..429061 (+) 540 WP_072324191.1 5-formyltetrahydrofolate cyclo-ligase -
  LK336_RS02225 (LK336_02225) - 429073..430536 (+) 1464 WP_031787264.1 rhomboid family intramembrane serine protease -
  LK336_RS02230 (LK336_02230) - 430517..430717 (+) 201 WP_000087563.1 YqgQ family protein -
  LK336_RS02235 (LK336_02235) - 430717..431703 (+) 987 WP_000161316.1 ROK family glucokinase -
  LK336_RS02240 (LK336_02240) - 431703..432032 (+) 330 WP_001018874.1 MTH1187 family thiamine-binding protein -
  LK336_RS02245 (LK336_02245) - 432029..432652 (+) 624 WP_031787263.1 MBL fold metallo-hydrolase -
  LK336_RS02250 (LK336_02250) comGA 432704..433678 (+) 975 WP_031787262.1 competence type IV pilus ATPase ComGA Machinery gene
  LK336_RS02255 (LK336_02255) comGB 433650..434720 (+) 1071 WP_031787261.1 competence type IV pilus assembly protein ComGB Machinery gene
  LK336_RS02260 (LK336_02260) comGC 434735..435046 (+) 312 WP_000921699.1 competence type IV pilus major pilin ComGC Machinery gene
  LK336_RS02265 (LK336_02265) comGD 435024..435470 (+) 447 WP_072324131.1 competence type IV pilus minor pilin ComGD Machinery gene
  LK336_RS02270 (LK336_02270) comGE 435457..435756 (+) 300 WP_031787259.1 hypothetical protein Machinery gene
  LK336_RS02275 (LK336_02275) comGF 435716..436171 (+) 456 WP_078097699.1 competence type IV pilus minor pilin ComGF Machinery gene
  LK336_RS02280 (LK336_02280) - 436270..436419 (+) 150 WP_072324192.1 hypothetical protein -
  LK336_RS02285 (LK336_02285) - 436406..436930 (+) 525 WP_031787256.1 shikimate kinase -
  LK336_RS02290 (LK336_02290) gcvT 437087..438178 (+) 1092 WP_000093342.1 glycine cleavage system aminomethyltransferase GcvT -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36867.03 Da        Isoelectric Point: 9.0234

>NTDB_id=625527 LK336_RS02250 WP_031787262.1 432704..433678(+) (comGA) [Staphylococcus argenteus strain RIVM_M036020]
MKILFQEIINKAIVMKASDVHFIPVHNEVSIKFRINDNLEQYEEIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHF
NKIYFLRISTLPLSLGQESCVIRIVPQYFKQTKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNEKAGINYVNSFKAILRCDPDVILIGEIRDKEVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLQYFFSHNHSLPTSFKKLETKLDDMTKAGVICESTM
DKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=625527 LK336_RS02250 WP_031787262.1 432704..433678(+) (comGA) [Staphylococcus argenteus strain RIVM_M036020]
ATGAAGATATTATTTCAAGAGATAATCAACAAAGCGATAGTAATGAAAGCGAGTGATGTCCATTTTATTCCTGTTCATAA
TGAAGTGAGTATTAAATTTCGTATAAATGACAATTTGGAACAGTATGAAGAAATTGGAAATAGTATTTATCAAAAGCTAT
TAGTTTATATGAAATTTCAAGCTGGTCTCGATGTATCTACGCAACAAGTTGCTCAGAGTGGTCGTTATAGCTACCATTTT
AATAAAATTTATTTTTTAAGAATTTCAACATTGCCATTATCACTTGGTCAAGAAAGTTGTGTGATAAGAATAGTTCCTCA
ATATTTTAAACAAACTAAATCAACATATAAATTTAATGATTTTAAACATTTAATGAATAAGAAACAAGGCTTATTGTTAT
TTAGTGGTCCGACGGGTTCAGGTAAAAGTACTTTAATGTATCAAATGGTCTCATACGCTAACAAAGCGTTAAACTTAAAT
GTTATATCTATTGAAGACCCAGTAGAAATGCAAATTCCTGGAATAGTTCAAATAAACGTAAATGAAAAAGCTGGGATTAA
CTATGTCAATTCATTTAAAGCGATATTAAGGTGTGACCCAGATGTTATTCTTATAGGGGAAATTAGAGATAAAGAAGTAG
CTAAATGTGTCATCCAAGCAAGTTTAAGTGGTCATCTAGTACTCACTACCTTACATGCAACAGATTGTAAAGGTGCAATT
TTAAGATTACTAGAAATGGGGATTTCAGTACAAGAACTAATACAAGCAACTAATTTAATTATAAATCAAAGACTTGTTAC
TACAATTAAACAGCAACGACAATTAGTGTGTGAAATTTTATCACAACAACAACTTCAGTATTTCTTCTCGCATAATCATT
CATTACCTACCTCATTTAAAAAACTAGAAACAAAGCTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAAGTACAATG
GATAAGTATATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7U7JUV7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

95.988

100

0.96
  comGA Staphylococcus aureus N315

95.988

100

0.96