Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LGV81_RS14385 Genome accession   NZ_CP086265
Coordinates   3033602..3035329 (+) Length   575 a.a.
NCBI ID   WP_020832718.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS647     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3028602..3040329
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LGV81_RS14370 (LGV81_14350) - 3028977..3029636 (-) 660 WP_265069747.1 DNA-binding protein -
  LGV81_RS14375 (LGV81_14355) - 3030662..3031771 (-) 1110 WP_265069748.1 site-specific integrase -
  LGV81_RS14380 (LGV81_14360) - 3032077..3033354 (+) 1278 WP_016723792.1 HlyC/CorC family transporter -
  LGV81_RS14385 (LGV81_14365) pilF 3033602..3035329 (+) 1728 WP_020832718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LGV81_RS14390 (LGV81_14370) pilC 3035374..3036639 (+) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  LGV81_RS14395 (LGV81_14375) - 3036642..3037523 (+) 882 WP_029240316.1 A24 family peptidase -
  LGV81_RS14400 (LGV81_14380) coaE 3037536..3038150 (+) 615 WP_019718221.1 dephospho-CoA kinase -
  LGV81_RS14405 (LGV81_14385) zapD 3038268..3039026 (+) 759 WP_016723794.1 cell division protein ZapD -
  LGV81_RS14410 (LGV81_14390) yacG 3039037..3039252 (+) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  LGV81_RS14415 (LGV81_14395) - 3039245..3039706 (-) 462 WP_023470258.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63754.56 Da        Isoelectric Point: 7.1546

>NTDB_id=624125 LGV81_RS14385 WP_020832718.1 3033602..3035329(+) (pilF) [Ralstonia pseudosolanacearum strain RS647]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDVDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=624125 LGV81_RS14385 WP_020832718.1 3033602..3035329(+) (pilF) [Ralstonia pseudosolanacearum strain RS647]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATGTCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAATATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.747

94.957

0.501
  pilB Acinetobacter baumannii D1279779

49.564

99.652

0.494
  pilB Acinetobacter baylyi ADP1

49.376

97.565

0.482
  pilB Legionella pneumophila strain ERS1305867

46.986

98.087

0.461
  pilB Vibrio cholerae strain A1552

44.643

97.391

0.435
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

94.261

0.424
  pilB Vibrio campbellii strain DS40M4

44.343

95.304

0.423
  pilF Thermus thermophilus HB27

38.256

97.739

0.374