Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   M5T00_RS01820 Genome accession   NZ_CP103589
Coordinates   400496..401734 (+) Length   412 a.a.
NCBI ID   WP_000816006.1    Uniprot ID   -
Organism   Escherichia coli strain 4750     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 395496..406734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T00_RS01795 (M5T00_01795) mrcA 395776..398328 (-) 2553 WP_001298208.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -
  M5T00_RS01800 (M5T00_01800) hofM 398448..399227 (+) 780 WP_001296474.1 DNA utilization protein HofM -
  M5T00_RS01805 (M5T00_01805) hofN 399227..399766 (+) 540 WP_001069307.1 DNA utilization protein HofN -
  M5T00_RS01810 (M5T00_01810) hofO 399750..400190 (+) 441 WP_001055744.1 DNA utilization protein HofO -
  M5T00_RS01815 (M5T00_01815) hofP 400180..400584 (+) 405 WP_001264138.1 DNA utilization protein HofP -
  M5T00_RS01820 (M5T00_01820) comE 400496..401734 (+) 1239 WP_000816006.1 DNA uptake porin HofQ Machinery gene
  M5T00_RS01825 (M5T00_01825) aroK 402135..402656 (+) 522 WP_000818618.1 shikimate kinase AroK -
  M5T00_RS01830 (M5T00_01830) aroB 402713..403801 (+) 1089 WP_001356903.1 3-dehydroquinate synthase -
  M5T00_RS01835 (M5T00_01835) damX 403893..405179 (+) 1287 WP_000343176.1 cell division protein DamX -
  M5T00_RS01840 (M5T00_01840) dam 405286..406122 (+) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44797.26 Da        Isoelectric Point: 5.9488

>NTDB_id=623353 M5T00_RS01820 WP_000816006.1 400496..401734(+) (comE) [Escherichia coli strain 4750]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=623353 M5T00_RS01820 WP_000816006.1 400496..401734(+) (comE) [Escherichia coli strain 4750]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTGTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGTTGGCCGATGCGCAACAAGGTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGCATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTACTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II and III secretion system protein (Secretin)

(250-409)

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  comE Glaesserella parasuis strain SC1401

35.782

100

0.367
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367