Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   M5T42_RS03790 Genome accession   NZ_CP103465
Coordinates   777358..778851 (+) Length   497 a.a.
NCBI ID   WP_000249373.1    Uniprot ID   -
Organism   Escherichia coli O25b:H4-ST131 strain 5392     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 772358..783851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5T42_RS03770 (M5T42_03770) pilD 773006..773815 (+) 810 WP_021538963.1 prepilin peptidase PppA Machinery gene
  M5T42_RS03775 (M5T42_03775) gspS2 773881..774291 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  M5T42_RS03780 (M5T42_03780) gspC 774309..775268 (+) 960 WP_024244404.1 type II secretion system protein GspC -
  M5T42_RS03785 (M5T42_03785) gspD 775298..777358 (+) 2061 WP_060624724.1 type II secretion system secretin GspD -
  M5T42_RS03790 (M5T42_03790) pilB/pilB1 777358..778851 (+) 1494 WP_000249373.1 type II secretion system ATPase GspE Machinery gene
  M5T42_RS03795 (M5T42_03795) gspF 778851..780074 (+) 1224 WP_001173427.1 type II secretion system inner membrane protein GspF -
  M5T42_RS03800 (M5T42_03800) gspG 780091..780546 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  M5T42_RS03805 (M5T42_03805) gspH 780550..781113 (+) 564 WP_001115120.1 type II secretion system minor pseudopilin GspH -
  M5T42_RS03810 (M5T42_03810) gspI 781110..781481 (+) 372 WP_000820073.1 type II secretion system minor pseudopilin GspI -
  M5T42_RS03815 (M5T42_03815) gspJ 781478..782083 (+) 606 WP_060624723.1 type II secretion system minor pseudopilin GspJ -
  M5T42_RS03820 (M5T42_03820) gspK 782083..783060 (+) 978 WP_001357646.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55194.27 Da        Isoelectric Point: 6.0089

>NTDB_id=622198 M5T42_RS03790 WP_000249373.1 777358..778851(+) (pilB/pilB1) [Escherichia coli O25b:H4-ST131 strain 5392]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNASFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=622198 M5T42_RS03790 WP_000249373.1 777358..778851(+) (pilB/pilB1) [Escherichia coli O25b:H4-ST131 strain 5392]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGTCTTTTACGCTGAGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCCTCC
GAGGCTCGCCAGTTGATGGAAGATCTCGGCTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGCGACGACGACGCGCCAATCATCAAACTGATCAACGCTATGCTGGCAGAGGCGATTAAAGAAGGTGCTT
CGGATATCCACATCGAGACATTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCGCGGCTGGATATTGCCGAAAAGCGCGTACC
GCAGGATGGGCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCACGAACTGACC
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACAGGTTCCGGCAAAAGCACCAC
GCTGTATGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACACAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTCCTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGAACCGTCACGCTGTACCATCCGCAGGGTTGTCCCGCCTG
TAATCATAAAGGTTTTCGCGGACGTACTGCCATCCATGAGCTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Legionella pneumophila strain ERS1305867

42.398

93.964

0.398
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362