Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   LJY22_RS13000 Genome accession   NZ_CP085843
Coordinates   2828313..2828906 (-) Length   197 a.a.
NCBI ID   WP_257896998.1    Uniprot ID   -
Organism   Vibrio diabolicus strain NV27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2823313..2833906
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY22_RS12980 (LJY22_12970) aroB 2824195..2825295 (-) 1101 WP_257896997.1 3-dehydroquinate synthase -
  LJY22_RS12985 (LJY22_12975) aroK 2825328..2825846 (-) 519 WP_005381319.1 shikimate kinase AroK -
  LJY22_RS12990 (LJY22_12980) pilQ 2826034..2827776 (-) 1743 WP_243328462.1 type IV pilus secretin PilQ Machinery gene
  LJY22_RS12995 (LJY22_12985) pilP 2827808..2828323 (-) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  LJY22_RS13000 (LJY22_12990) pilO 2828313..2828906 (-) 594 WP_257896998.1 type 4a pilus biogenesis protein PilO Machinery gene
  LJY22_RS13005 (LJY22_12995) pilN 2828899..2829474 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  LJY22_RS13010 (LJY22_13000) pilM 2829458..2830474 (-) 1017 WP_005395718.1 type IV pilus biogenesis protein PilM Machinery gene
  LJY22_RS13015 (LJY22_13005) - 2830618..2833179 (+) 2562 WP_257896999.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 23076.47 Da        Isoelectric Point: 4.7906

>NTDB_id=620823 LJY22_RS13000 WP_257896998.1 2828313..2828906(-) (pilO) [Vibrio diabolicus strain NV27]
MVDWQDLEIDEMAEWPLLPQLVVIALLIFLIQGFGYWFYLKPKEEQLNNLVQQEQTFKVALKIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGEKQSEHFLYRLPLNIELTGDFHDIGHFSQAIATLPRII
TFKDVTWQRVSQESETLHFRVRANTYQFKPEETNNDK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=620823 LJY22_RS13000 WP_257896998.1 2828313..2828906(-) (pilO) [Vibrio diabolicus strain NV27]
ATGGTTGATTGGCAGGATCTCGAAATAGATGAAATGGCAGAGTGGCCATTGTTGCCTCAGCTTGTGGTTATCGCGCTGCT
GATTTTCCTCATTCAAGGTTTTGGCTATTGGTTTTACCTTAAGCCAAAAGAAGAGCAATTGAATAACTTGGTCCAGCAGG
AGCAGACGTTCAAGGTAGCACTGAAGATCAAAGCGAACAAAGTAGCTGCATTGCCTCAGCTTCAATCTCAATTGGATGAA
CTCACCACGCGTTACGAGTTATTGCTACAACAACTGCCCGCGCAAAAAGAGTTGGCTACGATGCTCGCTTCGGTGAATGA
ACTCGGTATCGACAATAAGTTGACCTTTACCCGTATCGACTGGGGTGAAAAGCAATCAGAGCATTTCCTCTATCGTCTTC
CTTTAAACATTGAGTTAACGGGTGATTTTCATGACATTGGACATTTTTCTCAGGCGATAGCAACGCTACCTCGAATCATC
ACGTTCAAAGATGTGACTTGGCAGCGTGTCAGCCAAGAAAGTGAAACTCTGCACTTTAGGGTACGAGCCAATACCTATCA
GTTTAAGCCAGAGGAGACGAACAATGACAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

88.421

96.447

0.853
  pilO Vibrio cholerae strain A1552

70.051

100

0.701