Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   LJY22_RS13010 Genome accession   NZ_CP085843
Coordinates   2829458..2830474 (-) Length   338 a.a.
NCBI ID   WP_005395718.1    Uniprot ID   A0AAE4S9C7
Organism   Vibrio diabolicus strain NV27     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2824458..2835474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJY22_RS12985 (LJY22_12975) aroK 2825328..2825846 (-) 519 WP_005381319.1 shikimate kinase AroK -
  LJY22_RS12990 (LJY22_12980) pilQ 2826034..2827776 (-) 1743 WP_243328462.1 type IV pilus secretin PilQ Machinery gene
  LJY22_RS12995 (LJY22_12985) pilP 2827808..2828323 (-) 516 WP_005395715.1 pilus assembly protein PilP Machinery gene
  LJY22_RS13000 (LJY22_12990) pilO 2828313..2828906 (-) 594 WP_257896998.1 type 4a pilus biogenesis protein PilO Machinery gene
  LJY22_RS13005 (LJY22_12995) pilN 2828899..2829474 (-) 576 WP_046874634.1 PilN domain-containing protein Machinery gene
  LJY22_RS13010 (LJY22_13000) pilM 2829458..2830474 (-) 1017 WP_005395718.1 type IV pilus biogenesis protein PilM Machinery gene
  LJY22_RS13015 (LJY22_13005) - 2830618..2833179 (+) 2562 WP_257896999.1 penicillin-binding protein 1A -
  LJY22_RS13020 (LJY22_13010) oxyR 2833231..2834142 (-) 912 WP_164667088.1 DNA-binding transcriptional regulator OxyR -
  LJY22_RS13025 (LJY22_13015) - 2834313..2835041 (+) 729 WP_005395721.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37691.08 Da        Isoelectric Point: 6.9711

>NTDB_id=620825 LJY22_RS13010 WP_005395718.1 2829458..2830474(-) (pilM) [Vibrio diabolicus strain NV27]
MDKLIVTGIDIGHNSLKAVVLKPTGNNYALLGYKEILLKEGIVAENNTINHQEIVKTLKLIKKELPFGAKKVAISVPDNS
VISKKLQIEQNLEDSEVEFAVIQAFSHQSPFPVEELSLDFVRLRAEDGLRGTDNYQVFATRKDVVDSRVDALQKSGLKPV
LVDVHSQSLGHIWKLTADRFPDKNKYCLLDIGTSSSSFTMFTEQGELFHKEFACGTRGANSPVQDDLMSDDAQEKTELFN
RQIVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSQQLSLECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAIRGIHWLGGARALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=620825 LJY22_RS13010 WP_005395718.1 2829458..2830474(-) (pilM) [Vibrio diabolicus strain NV27]
ATGGATAAGCTAATCGTTACCGGCATCGATATAGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCACAGGCAACAA
TTACGCTCTGTTGGGTTACAAAGAAATATTACTAAAGGAAGGTATTGTCGCTGAAAATAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCTGATTAAAAAAGAACTGCCTTTTGGTGCTAAAAAGGTCGCAATTTCAGTGCCTGATAATTCG
GTGATCAGTAAAAAACTGCAAATAGAACAAAATCTTGAAGATAGCGAAGTTGAGTTTGCTGTGATTCAGGCATTTTCTCA
TCAATCGCCGTTCCCTGTCGAAGAGCTGAGTTTAGACTTCGTCCGTCTTCGTGCCGAAGATGGGTTGCGTGGGACTGATA
ACTATCAAGTGTTTGCGACAAGAAAAGATGTGGTCGATAGTCGCGTGGATGCATTACAAAAATCCGGACTAAAACCCGTT
TTAGTGGACGTGCACTCACAAAGTCTTGGTCATATCTGGAAGCTCACCGCCGATCGCTTTCCTGACAAAAATAAGTACTG
CTTACTCGATATTGGCACCTCATCGAGCTCCTTTACCATGTTTACCGAACAAGGAGAGCTGTTTCACAAAGAGTTTGCTT
GCGGCACTCGAGGAGCAAACAGCCCTGTGCAAGATGATTTGATGTCCGATGATGCTCAGGAGAAAACAGAGCTGTTTAAT
CGCCAAATCGTGGAACGGGTGAAACGTCAGATGCAGCTCTACACCTCAATTAATGGCTCGCAAAATATCAAAGGTATCTG
GTTATCAGGGGAAGGAGCTTCGACACCAATGCTCGCGGAAGAGTTATCTCAGCAACTGTCACTAGAGTGTGAATTGCTAA
ACCCGCTAGGGCTATTTGAGATGAAGGTGTCCAAACGTAAACGTCGTGCCGCGGATTGGCAGCATTTCTCTACAGCCGCA
GGACTCGCGATTCGTGGCATTCATTGGCTAGGAGGTGCGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

82.544

100

0.825
  pilM Vibrio cholerae strain A1552

50.746

99.112

0.503