Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LJU29_RS11565 Genome accession   NZ_CP085684
Coordinates   2637229..2638398 (+) Length   389 a.a.
NCBI ID   WP_257112391.1    Uniprot ID   -
Organism   Pseudomonas ficuserectae strain ZJDX-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2632229..2643398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LJU29_RS11550 - 2632666..2634993 (+) 2328 WP_257112386.1 ATP-dependent DNA helicase -
  LJU29_RS11555 pgm 2635109..2636755 (+) 1647 WP_257112387.1 phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent) -
  LJU29_RS11560 - 2636830..2637105 (-) 276 WP_002553334.1 peptidylprolyl isomerase -
  LJU29_RS11565 pilU 2637229..2638398 (+) 1170 WP_257112391.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LJU29_RS11570 - 2638447..2640024 (-) 1578 WP_257112715.1 ABC transporter ATP-binding protein -
  LJU29_RS11575 - 2640026..2641048 (-) 1023 WP_257112716.1 ABC transporter permease -
  LJU29_RS11580 - 2641048..2642112 (-) 1065 WP_005747402.1 microcin C ABC transporter permease YejB -

Sequence

Protein


Download         Length: 389 a.a.        Molecular weight: 42585.78 Da        Isoelectric Point: 6.1202

>NTDB_id=619289 LJU29_RS11565 WP_257112391.1 2637229..2638398(+) (pilU) [Pseudomonas ficuserectae strain ZJDX-003]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKRGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide


Download         Length: 1170 bp        

>NTDB_id=619289 LJU29_RS11565 WP_257112391.1 2637229..2638398(+) (pilU) [Pseudomonas ficuserectae strain ZJDX-003]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTCTCCACCGGAGCACCTCCGTG
CGCCAAATTCAACGGTGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTGGCGGTGATTGCTCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCGCGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAGTCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAACCAGCGCGAAGTGGGGGTCGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTACGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
ACTGGTCAGAACCCCGGACGGCAAACGCCGCGCAGCGGTGGAAGTGATGATGGGCACGCCGACCATTCGCGACCTGATTC
AGCGCAATGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.287

93.059

0.542
  pilU Acinetobacter baylyi ADP1

51.821

91.774

0.476
  pilU Vibrio cholerae strain A1552

52

89.974

0.468
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.948

98.458

0.393
  pilT Pseudomonas aeruginosa PAK

41.399

88.175

0.365