Detailed information    

insolico Bioinformatically predicted

Overview

Name   stkP   Type   Regulator
Locus tag   SIR_RS16330 Genome accession   NC_022246
Coordinates   1353648..1355522 (-) Length   624 a.a.
NCBI ID   WP_003073669.1    Uniprot ID   A0A3R9JBZ9
Organism   Streptococcus intermedius B196     
Function   require for competence development; phosphorylate ComE (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1341623..1396909 1353648..1355522 within 0


Gene organization within MGE regions


Location: 1341623..1396909
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS16260 (SIR_1280) - 1341804..1342523 (-) 720 WP_020998980.1 hypothetical protein -
  SIR_RS16265 (SIR_1281) - 1342774..1343028 (-) 255 WP_003073643.1 DUF1912 family protein -
  SIR_RS16270 (SIR_1282) - 1343039..1344400 (-) 1362 WP_021003038.1 DUF438 domain-containing protein -
  SIR_RS16275 (SIR_1283) - 1344400..1344633 (-) 234 WP_021003039.1 DUF1858 domain-containing protein -
  SIR_RS16280 (SIR_1284) rlmD 1344998..1346377 (-) 1380 WP_021003040.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  SIR_RS16285 (SIR_1285) - 1346434..1347180 (+) 747 WP_021003041.1 aminoglycoside 3'-phosphotransferase -
  SIR_RS16290 (SIR_1286) recX 1347274..1348050 (+) 777 WP_041787503.1 recombination regulator RecX -
  SIR_RS16295 (SIR_1287) - 1348137..1348670 (+) 534 WP_003073655.1 DUF402 domain-containing protein -
  SIR_RS16300 (SIR_1288) - 1348790..1349107 (+) 318 WP_003073656.1 DUF960 domain-containing protein -
  SIR_RS16305 (SIR_1289) - 1349227..1349619 (-) 393 WP_014829583.1 S1 RNA-binding domain-containing protein -
  SIR_RS16310 (SIR_1290) - 1349621..1351021 (-) 1401 WP_021003044.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  SIR_RS16315 (SIR_1291) - 1351061..1351693 (-) 633 WP_009568138.1 response regulator transcription factor -
  SIR_RS16320 (SIR_1292) - 1351695..1352690 (-) 996 WP_009568137.1 sensor histidine kinase -
  SIR_RS16325 (SIR_1293) liaF 1352687..1353385 (-) 699 WP_009568136.1 cell wall-active antibiotics response protein LiaF -
  SIR_RS16330 (SIR_1294) stkP 1353648..1355522 (-) 1875 WP_003073669.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  SIR_RS16335 (SIR_1295) - 1355519..1356259 (-) 741 WP_003073671.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  SIR_RS16340 (SIR_1296) rsmB 1356278..1357591 (-) 1314 WP_021003045.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  SIR_RS16345 (SIR_1297) fmt 1357581..1358516 (-) 936 WP_041787505.1 methionyl-tRNA formyltransferase -
  SIR_RS16350 (SIR_1298) - 1358577..1360958 (-) 2382 WP_021003047.1 primosomal protein N' -
  SIR_RS16355 (SIR_1299) rpoZ 1361017..1361331 (-) 315 WP_003028415.1 DNA-directed RNA polymerase subunit omega -
  SIR_RS16360 (SIR_1300) gmk 1361357..1361983 (-) 627 WP_003078308.1 guanylate kinase -
  SIR_RS16365 (SIR_1301) - 1362165..1363772 (-) 1608 WP_003073681.1 ribonuclease Y -
  SIR_RS19730 (SIR_1302) - 1363988..1364461 (+) 474 Protein_1295 S-ribosylhomocysteine lyase -
  SIR_RS16370 (SIR_1303) - 1364518..1365555 (-) 1038 WP_185756690.1 ORF6N domain-containing protein -
  SIR_RS16375 (SIR_1304) - 1365548..1367092 (-) 1545 WP_021003050.1 recombinase family protein -
  SIR_RS16380 (SIR_1305) - 1367094..1368776 (-) 1683 WP_021003051.1 recombinase family protein -
  SIR_RS16385 (SIR_1306) - 1368777..1370447 (-) 1671 WP_021003052.1 recombinase family protein -
  SIR_RS16390 (SIR_1307) - 1370589..1370819 (-) 231 WP_021003053.1 DUF6870 family protein -
  SIR_RS16395 (SIR_1308) - 1371284..1371694 (-) 411 WP_004633823.1 sigma-70 family RNA polymerase sigma factor -
  SIR_RS16400 (SIR_1309) - 1371962..1372657 (-) 696 WP_004633825.1 hypothetical protein -
  SIR_RS16405 (SIR_1310) - 1372632..1373492 (-) 861 WP_021003054.1 hypothetical protein -
  SIR_RS16410 (SIR_1311) - 1373494..1374489 (-) 996 WP_021003055.1 hypothetical protein -
  SIR_RS20050 (SIR_1312) - 1374509..1374661 (-) 153 WP_004633833.1 hypothetical protein -
  SIR_RS16415 (SIR_1313) - 1374688..1376025 (-) 1338 WP_021003056.1 radical SAM protein -
  SIR_RS16420 - 1376127..1376303 (-) 177 WP_004633836.1 subtilosin A family bacteriocin -
  SIR_RS16425 - 1376532..1376726 (-) 195 WP_004633838.1 helix-turn-helix transcriptional regulator -
  SIR_RS16430 (SIR_1314) - 1376845..1377960 (-) 1116 WP_004633841.1 hypothetical protein -
  SIR_RS16435 (SIR_1315) - 1377966..1378670 (-) 705 WP_004633842.1 ATP-binding cassette domain-containing protein -
  SIR_RS20055 (SIR_1316) - 1378673..1378825 (-) 153 WP_004633833.1 hypothetical protein -
  SIR_RS16440 (SIR_1317) - 1378852..1380189 (-) 1338 WP_021003056.1 radical SAM protein -
  SIR_RS16445 (SIR_1318) - 1380291..1380467 (-) 177 WP_004633836.1 subtilosin A family bacteriocin -
  SIR_RS16450 (SIR_1319) - 1380833..1381183 (+) 351 WP_041787513.1 plasmid mobilization protein -
  SIR_RS16455 (SIR_1320) - 1381189..1382520 (+) 1332 WP_021003058.1 relaxase/mobilization nuclease domain-containing protein -
  SIR_RS16460 (SIR_1321) - 1382588..1383259 (-) 672 WP_021003059.1 hypothetical protein -
  SIR_RS16465 (SIR_1322) - 1383301..1392429 (-) 9129 WP_021003060.1 helicase-related protein -
  SIR_RS16470 (SIR_1324) - 1392795..1394111 (+) 1317 WP_021003062.1 IS110 family transposase -
  SIR_RS16475 (SIR_1325) - 1394235..1395947 (-) 1713 WP_021003063.1 DNA topoisomerase 3 -
  SIR_RS16480 (SIR_1326) - 1396044..1396736 (-) 693 Protein_1320 CD1107 family mobile element protein -

Sequence

Protein


Download         Length: 624 a.a.        Molecular weight: 67672.81 Da        Isoelectric Point: 9.6670

>NTDB_id=61893 SIR_RS16330 WP_003073669.1 1353648..1355522(-) (stkP) [Streptococcus intermedius B196]
MIQVGKIFAGRYRIIKQIGRGGMADVYLAKDLILDGEEVAVKVLRTNYQTDPIAVARFQREARAMADLDHPHIVRITDIG
EEEGQQYLAMEYVAGLDLKRYIKEHAPLSNEEAVRIMGQILLAMRLAHTHGIVHRDLKPQNVLLTPDGDAKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAVTIALQHFQKPLPSVIAENPNVP
QALENVVIKATAKKLTDRYQSVAEMYVDLSSCLSADRRNEKKLVFDDTVKADTKTLPKVSQNTLTSASKPTATSTKEAVD
NQTKPKPKPKKKRRLRTRYKVLLSAVVLVIAALVALVYLSPSNVKIPDVTGKTVAQARSAIEAAGLKVGKEKDEYSDSAK
KDSVIRTNPQAGTQRREGSTVDLIVSKGPKTFTMEDYSGQKKADAINSLVQKYKVSKGLIKIEEVESSDYAAGTVVSQTP
AAGSTYDLTSKNRITLKVAKTVSSVTMPSYIGSTYEFAYNNLTEVIGVQGANVEKRIVTTAPAGTQAHTIIKQSPEAKRP
LNLKNDRIVLYVYEPKTEESSTSESSSQSGSSSNKNSPSSSSSSSSGNDGSSSKTPDTSASASN

Nucleotide


Download         Length: 1875 bp        

>NTDB_id=61893 SIR_RS16330 WP_003073669.1 1353648..1355522(-) (stkP) [Streptococcus intermedius B196]
ATGATTCAAGTCGGCAAAATATTTGCCGGACGATATAGAATCATCAAACAAATCGGTCGAGGAGGCATGGCAGATGTTTA
CTTGGCGAAAGATTTGATTTTAGACGGGGAAGAAGTCGCAGTTAAGGTTCTAAGAACCAACTACCAGACAGATCCGATTG
CTGTAGCGCGTTTTCAAAGAGAGGCGCGAGCCATGGCAGATTTGGATCATCCTCATATTGTCCGGATTACCGACATTGGA
GAAGAAGAAGGCCAGCAATATCTAGCAATGGAGTATGTGGCTGGACTTGACTTGAAACGCTATATCAAAGAGCATGCTCC
TTTGTCCAATGAAGAAGCGGTGCGAATCATGGGACAAATCCTTTTGGCGATGCGTTTGGCGCATACGCATGGGATTGTTC
ACCGTGATTTAAAACCTCAAAATGTCCTTCTAACTCCAGATGGCGATGCAAAAGTAACTGACTTTGGAATTGCAGTAGCC
TTTGCAGAAACGAGTTTAACCCAGACAAATTCAATGCTGGGATCTGTACATTATCTATCACCAGAACAGGCACGTGGATC
AAAAGCAACAGTTCAAAGTGATATTTATGCTATGGGGATTATTTTCTATGAAATGCTGACAGGTCACATTCCTTATGATG
GGGATAGTGCTGTGACGATTGCACTGCAGCATTTCCAAAAACCCCTTCCTTCTGTAATTGCTGAAAATCCAAATGTTCCA
CAAGCATTAGAAAATGTGGTTATCAAGGCAACGGCAAAAAAATTAACAGATCGTTATCAATCTGTTGCAGAAATGTATGT
TGACTTATCTAGTTGTTTATCTGCTGATCGTCGAAATGAGAAGAAATTAGTTTTTGATGATACTGTTAAAGCAGACACTA
AGACGCTTCCTAAAGTATCACAGAATACGTTGACTTCAGCGTCTAAACCGACTGCTACATCTACAAAAGAAGCTGTCGAT
AATCAAACTAAGCCTAAACCAAAACCAAAGAAAAAACGTCGTTTAAGAACGCGTTATAAAGTGTTGTTGTCGGCAGTAGT
CTTAGTTATTGCAGCTTTGGTAGCTTTAGTCTATTTGAGTCCCTCAAATGTAAAAATACCAGATGTTACTGGAAAAACCG
TCGCGCAAGCACGTTCCGCGATTGAAGCTGCAGGTTTGAAAGTTGGCAAGGAGAAAGACGAATACAGTGATTCTGCTAAG
AAAGATTCTGTCATTCGTACGAATCCACAAGCGGGAACACAACGTCGCGAAGGAAGCACAGTAGATTTGATTGTCTCAAA
AGGGCCGAAAACATTTACTATGGAAGATTATAGCGGTCAGAAAAAAGCAGATGCTATCAACAGTTTGGTGCAAAAATACA
AAGTTTCAAAGGGCTTGATTAAAATTGAAGAAGTCGAAAGTAGTGACTATGCAGCAGGAACTGTTGTTAGTCAGACGCCA
GCAGCAGGTAGTACGTATGATTTGACTTCAAAAAATCGGATTACGTTAAAAGTAGCGAAGACGGTTTCAAGTGTTACAAT
GCCAAGTTATATAGGTTCCACTTATGAATTTGCCTATAATAACCTTACCGAAGTTATTGGCGTTCAAGGAGCTAATGTTG
AAAAACGAATAGTTACAACAGCTCCAGCAGGAACACAGGCACATACGATTATTAAACAAAGCCCAGAAGCAAAGAGGCCT
CTAAATTTAAAAAATGATCGTATTGTCTTATATGTATATGAGCCAAAAACTGAAGAATCATCTACATCTGAAAGTAGTTC
GCAATCTGGAAGCAGTTCAAATAAAAATAGTCCATCATCTAGTAGTTCGTCGAGTAGCGGTAACGATGGATCAAGTTCGA
AAACACCAGACACAAGCGCTAGTGCAAGTAATTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-227)

PASTA domain (PASTA)

(433-500)

PASTA domain (PASTA)

(505-573)

PASTA domain (PASTA)

(364-427)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3R9JBZ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  stkP Streptococcus pneumoniae TIGR4

66.381

93.429

0.62
  stkP Streptococcus pneumoniae D39

66.038

93.429

0.617
  stkP Streptococcus pneumoniae R6

66.038

93.429

0.617
  stkP Streptococcus pneumoniae Rx1

65.866

93.429

0.615
  pknB Streptococcus mutans UA159

59.658

93.75

0.559
  stkP/pknB Streptococcus salivarius strain HSISS4

59.294

86.218

0.511

Multiple sequence alignment