Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC/celB   Type   Machinery gene
Locus tag   SIR_RS13420 Genome accession   NC_022246
Coordinates   727985..730219 (+) Length   744 a.a.
NCBI ID   WP_021002682.1    Uniprot ID   T1ZCW6
Organism   Streptococcus intermedius B196     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 722985..735219
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SIR_RS19650 (SIR_0716) rpmG 723847..723996 (+) 150 WP_003034401.1 50S ribosomal protein L33 -
  SIR_RS13400 (SIR_0717) secG 724036..724269 (+) 234 WP_003024744.1 preprotein translocase subunit SecG -
  SIR_RS13405 (SIR_0718) rnr 724360..726496 (+) 2137 Protein_702 ribonuclease R -
  SIR_RS13410 (SIR_0719) smpB 726662..727129 (+) 468 WP_021002680.1 SsrA-binding protein SmpB -
  SIR_RS13415 (SIR_0720) comEA/celA/cilE 727297..728001 (+) 705 WP_021002681.1 helix-hairpin-helix domain-containing protein Machinery gene
  SIR_RS13420 (SIR_0721) comEC/celB 727985..730219 (+) 2235 WP_021002682.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  SIR_RS13425 (SIR_0722) - 730297..731010 (+) 714 WP_003072599.1 DUF805 domain-containing protein -
  SIR_RS13430 (SIR_0723) - 731450..732436 (+) 987 WP_021002683.1 Gfo/Idh/MocA family protein -
  SIR_RS13435 (SIR_0724) - 732461..733114 (+) 654 WP_021002684.1 uracil-DNA glycosylase -
  SIR_RS13440 (SIR_0725) - 733141..733611 (+) 471 WP_021002685.1 NUDIX hydrolase -
  SIR_RS13445 (SIR_0726) - 733623..734897 (+) 1275 WP_021002686.1 dihydroorotase -

Sequence

Protein


Download         Length: 744 a.a.        Molecular weight: 85356.36 Da        Isoelectric Point: 10.1892

>NTDB_id=61874 SIR_RS13420 WP_021002682.1 727985..730219(+) (comEC/celB) [Streptococcus intermedius B196]
MSQWIKIFPIKPIYIAFLLVWLYFAIYQSNWLAGVGLIFLLIRLSRIYSLKEWFTTFMILACFAVFFLVRRELANRKIKV
EAPPVRQVAVLPDTIKVNGDSLSFRGKAKGQTYQIYYKLKSKEEKLAFQNLSSLVTLTVEGEFESPEKQRNFSGFDYQAY
LKTQGIYRILKVDQILSSQDRVSFQPFERLSSWRRKALVFIKRNFPNPMSNYMTGLLFGALDTDFGEMNNLYSSLGIIHL
FALSGMQVGFFMEGFRKSLLRLGFTQEIVHKCQYPFSFFYAGMTGFSVSVVRSLIQKLLSQHGITKLDNFALTIMVLSLI
MPSFLLTAGGVLSCAYAFIISVLDFKGLTPYKKIIIESIVISLGILPILIFYFGEFQPWSILLTFVFSLIFDIVMLPGLT
IIFLVSPFIKLTQVNFLFECLESSIRWLASMFSRPVVLGKPNPLLLIAMLLVLAILYDIRQNKKWLIFLSLFLSLLFFVA
KFPLQNEITMIDVGKGDSIFMRDWRGSTVLIDVGGREEIRKKESWQERISSSNAERTLIPYLKSRGVDTIDTLVLTNPNS
DYAGDVLEVAKKFSIKKIFISRSSLNDADFLNKLKETRAFVHVVKQGDKLPIFDHHLQVLSGTNKNDQSLVLYGQFFRTR
FLFMSNLTEEDEVKLMQLYPKLKTDVLKVGQHGSQNSSSSKFLQQVRPVIALISTGKNNSSKSLSQETIERFDRLNTKIY
RTDKQGAIKFLGWTTWQLETVQQP

Nucleotide


Download         Length: 2235 bp        

>NTDB_id=61874 SIR_RS13420 WP_021002682.1 727985..730219(+) (comEC/celB) [Streptococcus intermedius B196]
ATGTCACAGTGGATTAAGATATTTCCGATCAAACCAATTTATATTGCTTTCTTACTTGTCTGGTTATATTTTGCAATCTA
TCAAAGCAATTGGTTGGCAGGAGTGGGATTGATCTTTCTGTTAATTCGTCTTTCTCGCATATATTCGCTAAAAGAATGGT
TTACAACTTTCATGATTCTCGCTTGTTTTGCTGTTTTTTTCCTTGTTCGTAGGGAATTGGCGAATCGGAAGATAAAAGTA
GAAGCTCCTCCTGTAAGACAAGTTGCGGTTTTACCTGATACAATTAAGGTAAATGGTGATTCGCTTTCTTTTCGTGGTAA
AGCAAAAGGACAGACATATCAAATTTACTACAAATTGAAATCAAAAGAAGAAAAGTTGGCTTTTCAAAATCTATCCAGTC
TTGTCACATTAACTGTTGAGGGGGAATTTGAATCTCCTGAAAAGCAGCGCAATTTTTCTGGTTTTGATTATCAAGCCTAT
CTAAAAACACAAGGGATTTATCGAATTTTAAAGGTGGATCAAATTTTGTCTAGCCAAGATAGGGTTAGCTTCCAACCGTT
TGAGCGGCTGTCTAGCTGGCGAAGAAAAGCATTGGTTTTCATTAAGAGAAATTTCCCAAATCCAATGAGCAATTATATGA
CAGGGCTTTTGTTCGGAGCTTTGGATACAGATTTTGGTGAAATGAATAACCTTTATTCGAGCTTGGGAATTATTCATTTA
TTTGCATTGTCTGGTATGCAAGTTGGTTTTTTTATGGAAGGGTTTCGTAAGTCACTGTTGAGATTGGGGTTTACGCAGGA
AATAGTTCATAAGTGTCAATATCCATTTTCTTTTTTTTATGCTGGAATGACAGGTTTTTCAGTATCCGTTGTACGGAGTT
TAATCCAGAAATTATTGTCACAACACGGCATTACTAAGTTAGATAATTTTGCTTTAACAATAATGGTGTTGTCCTTGATT
ATGCCCTCCTTTCTTTTAACAGCAGGAGGAGTACTTTCTTGTGCGTATGCTTTTATCATTAGCGTTTTAGATTTTAAAGG
CCTGACTCCTTATAAAAAGATTATTATAGAGAGTATTGTCATTTCGCTTGGCATTTTACCAATTTTAATCTTTTATTTCG
GAGAATTTCAGCCCTGGTCTATTTTATTGACTTTTGTTTTTTCGTTGATTTTCGATATAGTGATGTTACCGGGGCTAACG
ATAATTTTTCTTGTTTCCCCTTTCATAAAACTCACTCAAGTTAATTTTCTATTTGAATGCTTAGAAAGTAGCATTCGCTG
GTTAGCAAGTATGTTTAGCAGACCAGTCGTTCTTGGCAAACCTAATCCACTTTTGCTAATCGCTATGTTGCTTGTATTAG
CTATCTTGTATGATATTCGGCAAAATAAAAAATGGCTGATATTTCTTAGTCTGTTTCTTTCATTACTCTTTTTTGTAGCT
AAATTTCCTTTACAAAATGAAATCACAATGATTGATGTCGGGAAGGGAGATAGTATTTTTATGCGAGACTGGAGAGGGAG
CACTGTATTGATTGATGTTGGCGGACGTGAAGAAATTAGAAAAAAAGAAAGTTGGCAAGAACGTATAAGTAGTTCAAACG
CAGAGAGAACGCTGATTCCATATCTTAAAAGTCGTGGTGTAGATACGATTGATACTTTAGTCTTAACAAATCCAAATTCA
GATTATGCAGGAGATGTATTAGAAGTTGCTAAAAAGTTTTCGATAAAGAAAATTTTTATTTCCAGAAGTAGTTTGAATGA
TGCAGACTTTTTAAATAAATTAAAGGAGACAAGGGCGTTTGTTCATGTCGTAAAACAAGGAGACAAACTTCCTATTTTTG
ATCATCATTTGCAAGTTCTTTCTGGTACGAATAAGAATGATCAATCGCTAGTTTTATATGGTCAATTTTTTCGTACAAGG
TTTTTATTTATGAGCAATTTAACAGAAGAGGATGAAGTAAAGCTAATGCAACTTTATCCAAAACTAAAAACAGATGTCTT
GAAGGTTGGGCAACATGGATCCCAAAATTCATCAAGTTCTAAGTTCTTACAGCAAGTAAGACCAGTGATTGCCCTCATCT
CTACTGGGAAAAACAATTCATCCAAATCGCTAAGTCAAGAAACAATTGAGCGATTCGATCGGTTAAATACAAAGATCTAT
CGAACAGATAAACAGGGGGCTATTAAGTTTTTGGGTTGGACAACATGGCAATTAGAAACTGTTCAGCAACCATAG

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(31-175)

Competence protein (Competence)

(216-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB T1ZCW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC/celB Streptococcus mitis SK321

55.689

100

0.559
  comEC/celB Streptococcus mitis NCTC 12261

55.496

100

0.556
  comEC/celB Streptococcus pneumoniae TIGR4

54.485

100

0.547
  comEC/celB Streptococcus pneumoniae Rx1

53.949

100

0.542
  comEC/celB Streptococcus pneumoniae D39

53.949

100

0.542
  comEC/celB Streptococcus pneumoniae R6

53.949

100

0.542
  comEC Lactococcus lactis subsp. cremoris KW2

46.03

99.866

0.46

Multiple sequence alignment