Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SCR2_RS02225 Genome accession   NC_022245
Coordinates   434175..435476 (+) Length   433 a.a.
NCBI ID   WP_020997640.1    Uniprot ID   U2ZSE5
Organism   Streptococcus constellatus subsp. pharyngis C818     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 434175..444243 434175..435476 within 0


Gene organization within MGE regions


Location: 434175..444243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCR2_RS02225 (SCR2_0442) comFA/cflA 434175..435476 (+) 1302 WP_020997640.1 DEAD/DEAH box helicase Machinery gene
  SCR2_RS02230 (SCR2_0443) - 435473..436138 (+) 666 WP_006267632.1 ComF family protein -
  SCR2_RS02235 (SCR2_0444) hpf 436217..436759 (+) 543 WP_006267638.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SCR2_RS02240 (SCR2_0445) rsmD 436956..437495 (+) 540 WP_020997641.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SCR2_RS02245 (SCR2_0446) coaD 437485..437982 (+) 498 WP_006267255.1 pantetheine-phosphate adenylyltransferase -
  SCR2_RS02250 (SCR2_0447) sepM 437963..439018 (+) 1056 WP_020997642.1 SepM family pheromone-processing serine protease Regulator
  SCR2_RS02255 (SCR2_0448) - 439305..439862 (+) 558 WP_006267391.1 YutD family protein -
  SCR2_RS02260 (SCR2_0449) rlmN 439896..440978 (+) 1083 WP_006269861.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SCR2_RS02265 (SCR2_0450) - 440971..441489 (+) 519 WP_003070167.1 VanZ family protein -
  SCR2_RS02270 (SCR2_0451) cclA/cilC 441484..442158 (-) 675 WP_020997643.1 prepilin peptidase Machinery gene
  SCR2_RS02275 (SCR2_0452) - 442309..442836 (+) 528 WP_006267546.1 Dps family protein -
  SCR2_RS02280 (SCR2_0454) - 443053..444243 (-) 1191 WP_006267506.1 site-specific integrase -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49206.01 Da        Isoelectric Point: 8.5393

>NTDB_id=61808 SCR2_RS02225 WP_020997640.1 434175..435476(+) (comFA/cflA) [Streptococcus constellatus subsp. pharyngis C818]
MTELQDCLGRIFTKNQLSPELQLQAQTLTGMVEEKGRLSCNRCGQAIDKEKQQLPIGAYYCRSCLFLGRIRSDEHLYYFS
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLHGESEAYSRSPLVIATTHHLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKYLGQKKLVPKLEQFVKKQRKTGFPLLIFASEIKRGQEFAE
ILQNNFPNEKVDFVASTTENRLDIVEKFRRKEITILISTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSME
RPTGELIFFHDGTTMAIEKAIKEIREMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=61808 SCR2_RS02225 WP_020997640.1 434175..435476(+) (comFA/cflA) [Streptococcus constellatus subsp. pharyngis C818]
ATGACGGAATTACAAGATTGTTTAGGTCGTATTTTTACAAAAAATCAACTGTCACCAGAATTGCAATTGCAAGCACAAAC
CTTAACTGGAATGGTAGAAGAAAAAGGGAGGTTAAGCTGCAATCGCTGTGGACAAGCCATTGACAAAGAAAAACAGCAAC
TACCAATAGGTGCTTATTATTGCAGGTCTTGCTTGTTCTTAGGAAGGATCAGAAGCGATGAACATCTTTACTATTTTTCA
CAGGAAGAGTTTCCTAAAGCGAATGTCTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCTAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACGGGAGCCGGAAAGACGGAAATGATCTATCAGG
TGGTGGCACAAGTCATCAATCAAGGCGGAGCCGTCTGCTTAGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTAGATTTTACCTGTGATATTTCACTCCTGCACGGCGAATCAGAAGCATATTCCCGCAGTCCTCTCGTGAT
TGCCACCACACATCATCTTCTCAAATTTTATCAAGCATTTGATCTTCTTATCGTTGATGAAGTAGATGCCTTTCCTTATG
TGGACAATCCGATGCTTTATCATGCAGTTCATCAGGCAGTCAAAGTAGAGGGGACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGCTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCCAGACGTTTTCATGGCAATCCTTT
GATTGTTCCGCAAAAAATTTGGTTGGCGGATTTTCAAAAATATCTTGGTCAAAAGAAGTTGGTTCCTAAGTTGGAACAAT
TTGTTAAAAAGCAAAGAAAAACAGGTTTTCCTCTTCTCATTTTTGCTTCTGAGATTAAAAGAGGACAAGAATTTGCAGAG
ATTCTCCAAAACAATTTCCCAAATGAAAAAGTTGACTTTGTAGCCTCAACGACTGAAAATCGACTCGATATTGTAGAGAA
ATTTCGTCGAAAAGAAATCACAATCTTAATATCAACGACGATTCTGGAACGTGGCGTGACTTTTCCTTGTGTAGATGTTT
TTGTGGTGGAGGCCAACCACCGTTTGTTTAGTCGCAGCGCTTTGGTACAAATTGCTGGTCGTGTTGGTCGTAGTATGGAG
CGACCAACAGGCGAGTTAATCTTTTTTCATGATGGTACAACTATGGCGATAGAAAAAGCTATTAAAGAAATTCGGGAGAT
GAATCAGGAGGCTGGTTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-397)

DEAD/DEAH box helicase (DEAD)

(108-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2ZSE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae D39

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae R6

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae TIGR4

70.327

98.845

0.695
  comFA/cflA Streptococcus mitis NCTC 12261

70.423

98.383

0.693
  comFA/cflA Streptococcus mitis SK321

69.953

98.383

0.688
  comFA Lactococcus lactis subsp. cremoris KW2

55.025

91.917

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

39.171

100

0.393
  comFA Bacillus subtilis subsp. subtilis str. 168

39.268

94.688

0.372

Multiple sequence alignment