Detailed information NTG0061621

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: SCI_RS08820
• Genome accession: NC_022238
• Coordinates: 1786480..1786917 (-)
• Length: 145 a.a.
• NCBI ID: WP_003035329.1
• Uniprot ID: F9P803
• Organism: Streptococcus constellatus subsp. pharyngis C1050
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1781480..1791917

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SCI_RS08800 (SCI_1724)	glmS	1781684..1783498 (-)	1815	WP_020998064.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
SCI_RS08805 (SCI_1725)	-	1783889..1785088 (-)	1200	WP_006267970.1	acetate kinase	-
SCI_RS08810 (SCI_1726)	comYH	1785135..1786088 (-)	954	WP_020998065.1	class I SAM-dependent methyltransferase	Machinery gene
SCI_RS08815 (SCI_1727)	comGG	1786173..1786499 (-)	327	WP_020998066.1	competence type IV pilus minor pilin ComGG	-
SCI_RS08820 (SCI_1728)	comGF/cglF	1786480..1786917 (-)	438	WP_003035329.1	competence type IV pilus minor pilin ComGF	Machinery gene
SCI_RS08825 (SCI_1729)	comGE/cglE	1786901..1787194 (-)	294	WP_003035318.1	competence type IV pilus minor pilin ComGE	Machinery gene
SCI_RS08830 (SCI_1730)	comYD	1787166..1787594 (-)	429	WP_020999411.1	competence type IV pilus minor pilin ComGD	Machinery gene
SCI_RS08835 (SCI_1731)	comYC	1787554..1787871 (-)	318	WP_020998068.1	competence type IV pilus major pilin ComGC	Machinery gene
SCI_RS08840 (SCI_1732)	comYB	1787868..1788890 (-)	1023	WP_037592679.1	competence type IV pilus assembly protein ComGB	Machinery gene
SCI_RS08845 (SCI_1733)	comYA	1788832..1789773 (-)	942	WP_006268058.1	competence type IV pilus ATPase ComGA	Machinery gene
SCI_RS08850	-	1789843..1790208 (-)	366	WP_022525692.1	DUF1033 family protein	-
SCI_RS08855 (SCI_1734)	glnA	1790396..1791742 (-)	1347	WP_020998070.1	type I glutamate--ammonia ligase	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16865.57 Da
• Isoelectric Point: 10.6422

>NTDB_id=61621 SCI_RS08820 WP_003035329.1 1786480..1786917(-) (comGF/cglF) [Streptococcus constellatus subsp. pharyngis C1050]
MFKANKVKAFTLLEALVALFVISGSVLLFQSMTRLLASEVRARQHSEQREWLLFAHQLEAELIRSSFEKVENNRLYMKQD
GKTIAFGKSSGQDFRKTNANGKGYQPMIYNVKKAEISQKNELIHIRMTFKRGLEREFVYRVETKS

Nucleotide

• Length: 438 bp

>NTDB_id=61621 SCI_RS08820 WP_003035329.1 1786480..1786917(-) (comGF/cglF) [Streptococcus constellatus subsp. pharyngis C1050]
GTGTTCAAGGCAAATAAAGTGAAAGCTTTTACACTTTTAGAGGCTCTGGTTGCCCTCTTTGTAATCAGCGGGAGTGTGCT
ATTATTTCAATCGATGACGCGTCTTCTGGCTTCGGAGGTCCGAGCTCGACAACACAGTGAACAACGAGAATGGCTTTTGT
TTGCCCATCAATTAGAAGCGGAATTGATTCGTTCTTCATTTGAAAAAGTGGAGAATAATCGTTTATACATGAAGCAGGAC
GGTAAGACGATTGCATTTGGAAAGTCAAGTGGACAAGATTTCAGAAAAACCAATGCAAATGGGAAAGGCTATCAGCCTAT
GATTTATAATGTGAAAAAAGCAGAAATCTCACAGAAAAATGAGCTGATTCATATCAGAATGACATTTAAGCGAGGATTGG
AAAGGGAGTTCGTCTATCGTGTGGAAACAAAGAGTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: F9P803

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae R6	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae Rx1	66.429	96.552	0.641
comGF/cglF	Streptococcus pneumoniae D39	66.429	96.552	0.641
comGF/cglF	Streptococcus mitis NCTC 12261	65.714	96.552	0.634
comGF/cglF	Streptococcus mitis SK321	65.714	96.552	0.634
comYF	Streptococcus mutans UA159	43.972	97.241	0.428
comYF	Streptococcus mutans UA140	43.972	97.241	0.428
comGF	Lactococcus lactis subsp. cremoris KW2	42.963	93.103	0.4