Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SCI_RS02320 Genome accession   NC_022238
Coordinates   452582..453883 (+) Length   433 a.a.
NCBI ID   WP_020997640.1    Uniprot ID   U2ZSE5
Organism   Streptococcus constellatus subsp. pharyngis C1050     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 434024..467336 452582..453883 within 0


Gene organization within MGE regions


Location: 434024..467336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCI_RS02235 (SCI_0444) - 434462..435238 (-) 777 WP_006267458.1 aminoglycoside 3'-phosphotransferase -
  SCI_RS02240 (SCI_0445) rlmD 435295..436659 (+) 1365 WP_006267121.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
  SCI_RS02245 (SCI_0446) - 437517..438449 (-) 933 WP_006267664.1 nitronate monooxygenase -
  SCI_RS02250 (SCI_0447) - 438784..439017 (+) 234 WP_003070208.1 DUF1858 domain-containing protein -
  SCI_RS02255 (SCI_0448) - 439017..440378 (+) 1362 WP_006267202.1 DUF438 domain-containing protein -
  SCI_RS02260 (SCI_0449) - 440391..440645 (+) 255 WP_006267273.1 DUF1912 family protein -
  SCI_RS02265 (SCI_0451) - 441032..441610 (+) 579 WP_003070202.1 GNAT family N-acetyltransferase -
  SCI_RS02270 (SCI_0452) - 441607..442413 (+) 807 WP_020999313.1 hypothetical protein -
  SCI_RS02275 (SCI_0453) - 442567..445215 (+) 2649 WP_006267276.1 valine--tRNA ligase -
  SCI_RS02280 - 445223..445612 (+) 390 WP_006267502.1 hypothetical protein -
  SCI_RS11115 (SCI_0456) - 447106..448614 (-) 1509 WP_020999314.1 YSIRK signal domain/LPXTG anchor domain surface protein -
  SCI_RS10150 (SCI_0457) - 448753..449633 (-) 881 Protein_433 ISAs1 family transposase -
  SCI_RS10155 (SCI_0458) - 449810..450079 (-) 270 Protein_434 IS30 family transposase -
  SCI_RS10160 (SCI_0459) - 450184..450908 (-) 725 Protein_435 ISL3 family transposase -
  SCI_RS02310 (SCI_0460) cysK 450862..451791 (-) 930 WP_020997639.1 cysteine synthase A -
  SCI_RS02315 (SCI_0461) - 451890..452525 (-) 636 WP_003035211.1 YigZ family protein -
  SCI_RS02320 (SCI_0462) comFA/cflA 452582..453883 (+) 1302 WP_020997640.1 DEAD/DEAH box helicase Machinery gene
  SCI_RS02325 (SCI_0463) - 453880..454545 (+) 666 WP_006267632.1 ComF family protein -
  SCI_RS02330 (SCI_0464) hpf 454624..455166 (+) 543 WP_006267638.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  SCI_RS02335 (SCI_0465) rsmD 455363..455902 (+) 540 WP_020997641.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  SCI_RS02340 (SCI_0466) coaD 455892..456389 (+) 498 WP_006267255.1 pantetheine-phosphate adenylyltransferase -
  SCI_RS02345 (SCI_0467) sepM 456370..457425 (+) 1056 WP_020997642.1 SepM family pheromone-processing serine protease Regulator
  SCI_RS02350 (SCI_0468) - 457712..458269 (+) 558 WP_006267391.1 YutD family protein -
  SCI_RS02355 (SCI_0469) rlmN 458303..459385 (+) 1083 WP_006269861.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  SCI_RS02360 (SCI_0470) - 459378..459896 (+) 519 WP_003070167.1 VanZ family protein -
  SCI_RS02365 (SCI_0471) cclA/cilC 459891..460565 (-) 675 WP_020997643.1 A24 family peptidase Machinery gene
  SCI_RS02370 (SCI_0472) - 460716..461243 (+) 528 WP_006267546.1 DNA starvation/stationary phase protection protein -
  SCI_RS02375 (SCI_0474) - 461460..462650 (-) 1191 WP_006267506.1 site-specific integrase -
  SCI_RS02380 (SCI_0475) - 462726..462929 (-) 204 WP_002983016.1 excisionase -
  SCI_RS02385 (SCI_0476) - 463359..463610 (-) 252 WP_006267285.1 helix-turn-helix domain-containing protein -
  SCI_RS02390 (SCI_0477) - 463591..464034 (-) 444 WP_006267609.1 RNA polymerase sigma factor -
  SCI_RS02395 (SCI_0478) - 464375..465709 (-) 1335 WP_006267371.1 MATE family efflux transporter -
  SCI_RS10170 (SCI_0479) - 466343..466667 (+) 325 Protein_454 plasmid mobilization protein -
  SCI_RS10875 - 466634..466864 (+) 231 Protein_455 relaxase/mobilization nuclease domain-containing protein -
  SCI_RS10180 - 466836..466988 (+) 153 WP_080654533.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 433 a.a.        Molecular weight: 49206.01 Da        Isoelectric Point: 8.5393

>NTDB_id=61582 SCI_RS02320 WP_020997640.1 452582..453883(+) (comFA/cflA) [Streptococcus constellatus subsp. pharyngis C1050]
MTELQDCLGRIFTKNQLSPELQLQAQTLTGMVEEKGRLSCNRCGQAIDKEKQQLPIGAYYCRSCLFLGRIRSDEHLYYFS
QEEFPKANVLKWQGKLTEFQAKVSQGLVEAVTKRKDSLVHAVTGAGKTEMIYQVVAQVINQGGAVCLASPRIDVCLELYR
RLKVDFTCDISLLHGESEAYSRSPLVIATTHHLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVHQAVKVEGTKIFLTATS
TDELDKKVAKGELTRLSLPRRFHGNPLIVPQKIWLADFQKYLGQKKLVPKLEQFVKKQRKTGFPLLIFASEIKRGQEFAE
ILQNNFPNEKVDFVASTTENRLDIVEKFRRKEITILISTTILERGVTFPCVDVFVVEANHRLFSRSALVQIAGRVGRSME
RPTGELIFFHDGTTMAIEKAIKEIREMNQEAGL

Nucleotide


Download         Length: 1302 bp        

>NTDB_id=61582 SCI_RS02320 WP_020997640.1 452582..453883(+) (comFA/cflA) [Streptococcus constellatus subsp. pharyngis C1050]
ATGACGGAATTACAAGATTGTTTAGGTCGTATTTTTACAAAAAATCAACTGTCACCAGAATTGCAATTGCAAGCACAAAC
CTTAACTGGAATGGTAGAAGAAAAAGGGAGGTTAAGCTGCAATCGCTGTGGACAAGCCATTGACAAAGAAAAACAGCAAC
TACCAATAGGTGCTTATTATTGCAGGTCTTGCTTGTTCTTAGGAAGGATCAGAAGCGATGAACATCTTTACTATTTTTCA
CAGGAAGAGTTTCCTAAAGCGAATGTCTTGAAATGGCAAGGAAAGTTGACAGAATTTCAAGCTAAGGTTTCTCAAGGACT
TGTAGAGGCGGTTACCAAACGCAAAGATAGCTTGGTTCACGCAGTCACGGGAGCCGGAAAGACGGAAATGATCTATCAGG
TGGTGGCACAAGTCATCAATCAAGGCGGAGCCGTCTGCTTAGCTAGCCCCAGAATTGATGTCTGCTTAGAACTTTATCGC
AGACTGAAAGTAGATTTTACCTGTGATATTTCACTCCTGCACGGCGAATCAGAAGCATATTCCCGCAGTCCTCTCGTGAT
TGCCACCACACATCATCTTCTCAAATTTTATCAAGCATTTGATCTTCTTATCGTTGATGAAGTAGATGCCTTTCCTTATG
TGGACAATCCGATGCTTTATCATGCAGTTCATCAGGCAGTCAAAGTAGAGGGGACGAAGATTTTCTTAACAGCAACTTCC
ACAGATGAGCTGGATAAAAAAGTGGCTAAAGGAGAATTAACTCGTTTGAGTCTACCCAGACGTTTTCATGGCAATCCTTT
GATTGTTCCGCAAAAAATTTGGTTGGCGGATTTTCAAAAATATCTTGGTCAAAAGAAGTTGGTTCCTAAGTTGGAACAAT
TTGTTAAAAAGCAAAGAAAAACAGGTTTTCCTCTTCTCATTTTTGCTTCTGAGATTAAAAGAGGACAAGAATTTGCAGAG
ATTCTCCAAAACAATTTCCCAAATGAAAAAGTTGACTTTGTAGCCTCAACGACTGAAAATCGACTCGATATTGTAGAGAA
ATTTCGTCGAAAAGAAATCACAATCTTAATATCAACGACGATTCTGGAACGTGGCGTGACTTTTCCTTGTGTAGATGTTT
TTGTGGTGGAGGCCAACCACCGTTTGTTTAGTCGCAGCGCTTTGGTACAAATTGCTGGTCGTGTTGGTCGTAGTATGGAG
CGACCAACAGGCGAGTTAATCTTTTTTCATGATGGTACAACTATGGCGATAGAAAAAGCTATTAAAGAAATTCGGGAGAT
GAATCAGGAGGCTGGTTTATGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(292-397)

DEAD/DEAH box helicase (DEAD)

(108-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB U2ZSE5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae D39

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae R6

70.561

98.845

0.697
  comFA/cflA Streptococcus pneumoniae TIGR4

70.327

98.845

0.695
  comFA/cflA Streptococcus mitis NCTC 12261

70.423

98.383

0.693
  comFA/cflA Streptococcus mitis SK321

69.953

98.383

0.688
  comFA Lactococcus lactis subsp. cremoris KW2

55.025

91.917

0.506
  comFA Latilactobacillus sakei subsp. sakei 23K

39.171

100

0.393
  comFA Bacillus subtilis subsp. subtilis str. 168

39.268

94.688

0.372

Multiple sequence alignment