Detailed information NTG0061513

Bioinformatically predicted

Overview

• Name: ruvB
• Type: Machinery gene
• Locus tag: EKM72_RS01645
• Genome accession: NZ_AP019305
• Coordinates: 319812..320816 (-)
• Length: 334 a.a.
• NCBI ID: WP_001005768.1
• Uniprot ID: P66757
• Organism: Staphylococcus aureus strain TUM9458
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 314812..325816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EKM72_RS01630	yajC	317344..317604 (-)	261	WP_001160682.1	preprotein translocase subunit YajC	-
EKM72_RS01635	tgt	317623..318762 (-)	1140	WP_001112045.1	tRNA guanosine(34) transglycosylase Tgt	-
EKM72_RS01640	queA	318785..319810 (-)	1026	WP_001019178.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
EKM72_RS01645	ruvB	319812..320816 (-)	1005	WP_001005768.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
EKM72_RS01650	ruvA	320848..321450 (-)	603	WP_000271550.1	Holliday junction branch migration protein RuvA	Machinery gene
EKM72_RS01655	-	321464..321919 (-)	456	WP_000368902.1	ACT domain-containing protein	-
EKM72_RS01660	obgE	321932..323224 (-)	1293	WP_000496100.1	GTPase ObgE	-
EKM72_RS01665	rpmA	323578..323862 (-)	285	WP_000916187.1	50S ribosomal protein L27	-
EKM72_RS01670	-	323874..324194 (-)	321	WP_000633692.1	ribosomal-processing cysteine protease Prp	-
EKM72_RS01675	rplU	324200..324508 (-)	309	WP_000457386.1	50S ribosomal protein L21	-
EKM72_RS01680	mreD	324744..325274 (-)	531	WP_001259925.1	rod shape-determining protein MreD	-

Sequence

Protein

• Length: 334 a.a.
• Molecular weight: 37645.94 Da
• Isoelectric Point: 5.1876

>NTDB_id=61513 EKM72_RS01645 WP_001005768.1 319812..320816(-) (ruvB) [Staphylococcus aureus strain TUM9458]
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG

Nucleotide

• Length: 1005 bp

>NTDB_id=61513 EKM72_RS01645 WP_001005768.1 319812..320816(-) (ruvB) [Staphylococcus aureus strain TUM9458]
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
ATTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAATGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGTGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGGATAA

Domains

Predicted by InterProScan.

• RuvB AAA lid domain (AAA_lid_4) (182-255)

• RuvB C-terminal winged helix domain (WHD_RuvB) (257-326)

• Holliday junction DNA helicase RuvB P-loop domain (RuvB_N) (21-179)

3D structure

• AlphaFold DB: P66757

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ruvB	Bacillus subtilis subsp. subtilis str. 168	65.951	97.605	0.644
ruvB	Streptococcus pneumoniae TIGR4	58.182	98.802	0.575
ruvB	Streptococcus pneumoniae R6	57.879	98.802	0.572
ruvB	Streptococcus pneumoniae D39	57.879	98.802	0.572
ruvB	Helicobacter pylori 26695	56.481	97.006	0.548
ruvB	Synechocystis sp. PCC 6803	53.75	95.808	0.515