NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NP943_RS11260	Genome accession	NZ_CP102061
Coordinates	2304900..2306024 (-)	Length	374 a.a.
NCBI ID	WP_000228517.1	Uniprot ID	A0A0H3EME5
Organism	Escherichia coli strain C31
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 2299900..2311024

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NP943_RS11230 (NP943_11230)	yrdA	2301700..2302254 (+)	555	WP_000005712.1	gamma carbonic anhydrase family protein	-
NP943_RS11235 (NP943_11235)	yrdB	2302230..2302487 (-)	258	WP_001070565.1	DUF1488 domain-containing protein	-
NP943_RS11240 (NP943_11240)	aroE	2302484..2303302 (-)	819	WP_000451255.1	shikimate dehydrogenase	-
NP943_RS11245 (NP943_11245)	tsaC	2303307..2303879 (-)	573	WP_001296460.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NP943_RS11250 (NP943_11250)	yrdD	2303884..2304426 (-)	543	WP_001296461.1	type I DNA topoisomerase	-
NP943_RS11255 (NP943_11255)	smg	2304455..2304928 (-)	474	WP_000460673.1	DUF494 family protein Smg	-
NP943_RS11260 (NP943_11260)	dprA	2304900..2306024 (-)	1125	WP_000228517.1	DNA-protecting protein DprA	Machinery gene
NP943_RS11265 (NP943_11265)	def	2306154..2306663 (+)	510	WP_000114984.1	peptide deformylase	-
NP943_RS11270 (NP943_11270)	fmt	2306678..2307625 (+)	948	WP_000004421.1	methionyl-tRNA formyltransferase	-
NP943_RS11275 (NP943_11275)	rsmB	2307677..2308966 (+)	1290	WP_000744765.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NP943_RS11280 (NP943_11280)	trkA	2308988..2310364 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NP943_RS11285 (NP943_11285)	mscL	2310494..2310907 (+)	414	WP_000022450.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40910.86 Da Isoelectric Point: 6.0395

                                >NTDB_id=614595 NP943_RS11260 WP_000228517.1 2304900..2306024(-) (dprA) [Escherichia coli strain C31]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=614595 NP943_RS11260 WP_000228517.1 2304900..2306024(-) (dprA) [Escherichia coli strain C31]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTATGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGTTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTTAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCTCCACTGGCTGCCAGTCTACT

TGAGCAAGGTGGTGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAGGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCTTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H3EME5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.78	98.663	0.481
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	49.355	82.888	0.409
dprA	Vibrio cholerae strain A1552	49.355	82.888	0.409
dprA	Glaesserella parasuis strain SC1401	44.807	90.107	0.404
dprA	Haemophilus influenzae Rd KW20	44.214	90.107	0.398
dprA	Legionella pneumophila strain ERS1305867	44.242	88.235	0.39

Detailed information NTG0614595