NTDB

Overview

Name	comGF	Type	Machinery gene
Locus tag	LB315_RS08240	Genome accession	NZ_CP084892
Coordinates	1673870..1674367 (-)	Length	165 a.a.
NCBI ID	WP_001825340.1	Uniprot ID	A0A2S6DDC2
Organism	Staphylococcus aureus strain CC1956/ST4614-MSSA
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1668870..1679367

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LB315_RS08215 (LB315_08225)	gcvPB	1669032..1670504 (-)	1473	WP_000202180.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
LB315_RS08220 (LB315_08230)	gcvPA	1670497..1671843 (-)	1347	WP_000019687.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
LB315_RS08225 (LB315_08235)	gcvT	1671863..1672954 (-)	1092	WP_000093349.1	glycine cleavage system aminomethyltransferase GcvT	-
LB315_RS08230 (LB315_08240)	-	1673113..1673637 (-)	525	WP_001015120.1	shikimate kinase	-
LB315_RS08235 (LB315_08245)	-	1673627..1673773 (-)	147	WP_001789879.1	hypothetical protein	-
LB315_RS08240 (LB315_08250)	comGF	1673870..1674367 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
LB315_RS08245 (LB315_08255)	comGE	1674285..1674584 (-)	300	WP_000844413.1	hypothetical protein	Machinery gene
LB315_RS08250 (LB315_08260)	comGD	1674571..1675017 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
LB315_RS08255 (LB315_08265)	comGC	1674995..1675306 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
LB315_RS08260 (LB315_08270)	comGB	1675320..1676390 (-)	1071	WP_000776418.1	competence type IV pilus assembly protein ComGB	Machinery gene
LB315_RS08265 (LB315_08275)	comGA	1676362..1677336 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
LB315_RS08270 (LB315_08280)	-	1677388..1678011 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
LB315_RS08275 (LB315_08285)	-	1678008..1678337 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
LB315_RS08280 (LB315_08290)	-	1678337..1679323 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

Download Length: 165 a.a. Molecular weight: 19179.92 Da Isoelectric Point: 10.2192

                                >NTDB_id=613817 LB315_RS08240 WP_001825340.1 1673870..1674367(-) (comGF) [Staphylococcus aureus strain CC1956/ST4614-MSSA]

MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI

LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT

KTIYV

Nucleotide

Download Length: 498 bp

                                >NTDB_id=613817 LB315_RS08240 WP_001825340.1 1673870..1674367(-) (comGF) [Staphylococcus aureus strain CC1956/ST4614-MSSA]

ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT

TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT

TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT

CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT

GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA

CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT

AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A2S6DDC2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97

Detailed information NTG0613817