Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LG939_RS06180 Genome accession   NZ_CP084738
Coordinates   1310851..1312578 (-) Length   575 a.a.
NCBI ID   WP_020832718.1    Uniprot ID   -
Organism   Ralstonia pseudosolanacearum strain RS639     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1305851..1317578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LG939_RS06150 (LG939_06175) - 1306474..1306935 (+) 462 WP_023470258.1 NUDIX domain-containing protein -
  LG939_RS06155 (LG939_06180) yacG 1306928..1307143 (-) 216 WP_011002736.1 DNA gyrase inhibitor YacG -
  LG939_RS06160 (LG939_06185) zapD 1307154..1307912 (-) 759 WP_016723794.1 cell division protein ZapD -
  LG939_RS06165 (LG939_06190) coaE 1308030..1308644 (-) 615 WP_019718221.1 dephospho-CoA kinase -
  LG939_RS06170 (LG939_06195) - 1308657..1309538 (-) 882 WP_029240316.1 A24 family peptidase -
  LG939_RS06175 (LG939_06200) pilC 1309541..1310806 (-) 1266 WP_019718222.1 type II secretion system F family protein Machinery gene
  LG939_RS06180 (LG939_06205) pilF 1310851..1312578 (-) 1728 WP_020832718.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LG939_RS06185 (LG939_06210) - 1312826..1314103 (-) 1278 WP_016723792.1 HlyC/CorC family transporter -
  LG939_RS06195 (LG939_06220) ispB 1314391..1315359 (-) 969 WP_011002729.1 octaprenyl diphosphate synthase -
  LG939_RS06200 (LG939_06225) rplU 1315638..1315949 (+) 312 WP_011002728.1 50S ribosomal protein L21 -
  LG939_RS06205 (LG939_06230) rpmA 1315987..1316247 (+) 261 WP_003271630.1 50S ribosomal protein L27 -
  LG939_RS06210 (LG939_06235) obgE 1316424..1317524 (+) 1101 WP_011002727.1 GTPase ObgE -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 63754.56 Da        Isoelectric Point: 7.1546

>NTDB_id=613001 LG939_RS06180 WP_020832718.1 1310851..1312578(-) (pilF) [Ralstonia pseudosolanacearum strain RS639]
MTLGLSLAQSRRIAPTLLAQLEQSAREKRTQLIDELVSSGIMSAHDLAVFVAGKYQMPLLDLAQYKLDTVPPVLSANRHF
QQLRLLPLGRRDNRIIVATSDPSDPKPLEELRQKMNVAIETVIVEHDKLVRQLSLSNEAPAEIKSLIPKQETTQIEYDPG
AAAAVRRTPTDGIDDAPVVRFLQKLFTEALLRGTSDLHFEPFETFYRVRFRIDGILEQVAQPPLDIRDKIATRIKVLSRL
DISEKRVPQDGRMKLLITVPKDKNDKDNKEMVERAIDFRVSTLPTLFGEKIVIRILESSTERLDVDQLGYEPEQKQLLLD
VIKRPYGMVLVTGPTGSGKTVSLYTFLNRLNQGDINISTAEDPAEIQLPGINQVNVNDKAGLTFAAALKSFLRQDPDIIM
VGEIRDLETADISIKAAQTGHLVFSTLHTNDAPTTLTRLMNMGVAPFNIASSVLMITAQRLGRRLCKACKKPGPLPKETL
LDAGFKEADLDGSWQPYHPVGCEACNGSGYKGRVGIYQVMPITEAIQEIILAHGTALQIAEQARKEGVLSLRDSGLRKVK
QGLTSLEEVLATTNQ

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=613001 LG939_RS06180 WP_020832718.1 1310851..1312578(-) (pilF) [Ralstonia pseudosolanacearum strain RS639]
ATGACTCTCGGACTCTCTCTTGCGCAGAGCCGGCGAATTGCGCCGACATTACTCGCCCAGCTGGAACAAAGCGCCCGCGA
AAAGCGCACGCAGCTGATCGACGAACTGGTGAGCAGCGGCATCATGAGCGCTCATGATCTCGCCGTCTTCGTGGCGGGCA
AATACCAGATGCCGCTGCTGGACCTGGCGCAATACAAGCTCGACACCGTGCCGCCCGTGCTGTCGGCCAACAGGCATTTT
CAGCAGTTGCGCCTGTTGCCGCTGGGCCGGCGTGACAACCGCATCATCGTGGCCACTTCGGACCCGAGCGACCCCAAGCC
GCTCGAGGAACTGCGCCAGAAGATGAACGTGGCGATCGAGACGGTCATCGTCGAGCACGACAAGCTGGTGCGCCAGCTGA
GCCTCTCCAACGAGGCACCGGCCGAGATCAAGTCGCTCATCCCCAAGCAGGAAACCACGCAGATCGAATACGACCCGGGT
GCGGCCGCGGCGGTCCGCCGCACCCCGACCGACGGCATCGACGATGCGCCGGTGGTGCGCTTCCTGCAGAAGCTGTTTAC
CGAGGCGCTGCTGCGCGGCACCTCCGACTTGCATTTCGAGCCGTTTGAAACGTTTTACCGCGTGCGCTTCCGCATCGACG
GCATCCTCGAGCAGGTCGCGCAGCCGCCGCTGGACATCCGCGACAAGATCGCCACCCGCATCAAGGTGCTGTCGCGCCTG
GACATATCGGAAAAGCGCGTGCCGCAGGACGGCCGCATGAAGCTCCTGATCACCGTTCCGAAAGACAAGAACGACAAGGA
CAACAAGGAGATGGTCGAGCGCGCCATCGACTTCCGGGTGTCGACGCTGCCGACGCTCTTCGGCGAGAAGATCGTGATCC
GGATTCTGGAGTCCTCGACCGAGCGGCTCGATGTCGACCAGCTCGGCTATGAGCCCGAGCAGAAGCAGCTCCTGCTGGAC
GTGATCAAGCGCCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTAGCGGCAAGACGGTGTCGCTGTACACCTTCCT
GAACAGGCTGAACCAGGGCGACATCAATATTTCCACCGCGGAAGACCCGGCGGAAATCCAGCTGCCCGGCATCAACCAGG
TCAACGTCAACGACAAGGCGGGGCTGACCTTCGCCGCGGCGCTGAAGTCCTTTCTGCGGCAGGATCCCGACATCATCATG
GTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCTCCATCAAGGCCGCGCAGACCGGCCACTTGGTGTTCTCCACGCT
GCACACCAACGACGCGCCGACCACGCTGACACGCCTGATGAACATGGGCGTGGCGCCGTTCAACATCGCATCGTCGGTGC
TGATGATCACGGCGCAGCGTCTGGGGCGGCGCCTGTGCAAGGCCTGCAAGAAGCCGGGTCCGCTGCCCAAGGAAACGCTG
CTGGACGCGGGCTTCAAGGAAGCAGACCTGGACGGCTCGTGGCAGCCGTACCATCCGGTCGGCTGCGAGGCATGCAACGG
CAGCGGCTACAAGGGCCGGGTCGGCATCTACCAGGTCATGCCGATCACCGAGGCGATCCAGGAGATCATCCTCGCCCACG
GCACGGCGCTGCAGATCGCCGAACAGGCGCGCAAGGAAGGCGTGCTCTCGCTGCGCGATTCCGGCCTGCGGAAAGTGAAG
CAGGGGCTGACTTCCCTTGAGGAAGTGTTGGCCACGACCAACCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(184-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(36-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

52.747

94.957

0.501
  pilB Acinetobacter baumannii D1279779

49.564

99.652

0.494
  pilB Acinetobacter baylyi ADP1

49.376

97.565

0.482
  pilB Legionella pneumophila strain ERS1305867

46.986

98.087

0.461
  pilB Vibrio cholerae strain A1552

44.643

97.391

0.435
  pilB Vibrio parahaemolyticus RIMD 2210633

45.018

94.261

0.424
  pilB Vibrio campbellii strain DS40M4

44.343

95.304

0.423
  pilF Thermus thermophilus HB27

38.256

97.739

0.374