Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   LA387_RS05680 Genome accession   NZ_CP084377
Coordinates   1109431..1111641 (-) Length   736 a.a.
NCBI ID   WP_017370462.1    Uniprot ID   -
Organism   Lactococcus garvieae strain Lg-Granada     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1109431..1155211 1109431..1111641 within 0


Gene organization within MGE regions


Location: 1109431..1155211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LA387_RS05680 comEC 1109431..1111641 (-) 2211 WP_017370462.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  LA387_RS05685 - 1111661..1112311 (-) 651 WP_004259187.1 helix-hairpin-helix domain-containing protein -
  LA387_RS05690 - 1112369..1113544 (-) 1176 WP_004259189.1 SLC13 family permease -
  LA387_RS05695 - 1113710..1115266 (-) 1557 WP_004259192.1 ABC-F family ATP-binding cassette domain-containing protein -
  LA387_RS05700 - 1115963..1116145 (+) 183 WP_225666403.1 hypothetical protein -
  LA387_RS05705 - 1116792..1117517 (-) 726 WP_225666405.1 CHAP domain-containing protein -
  LA387_RS05710 - 1117518..1117748 (-) 231 WP_225666407.1 holin -
  LA387_RS05715 - 1117752..1118078 (-) 327 WP_225666409.1 hypothetical protein -
  LA387_RS05720 - 1118111..1118308 (-) 198 WP_225666411.1 hypothetical protein -
  LA387_RS05725 - 1118402..1121398 (-) 2997 WP_225666413.1 hypothetical protein -
  LA387_RS05730 - 1121398..1122939 (-) 1542 WP_225666415.1 distal tail protein Dit -
  LA387_RS05735 - 1122939..1127870 (-) 4932 WP_225666417.1 phage tail tape measure protein -
  LA387_RS05740 - 1127952..1128443 (-) 492 WP_179107590.1 zinc ribbon domain-containing protein -
  LA387_RS05745 - 1128672..1129088 (-) 417 WP_021214451.1 phage tail assembly chaperone -
  LA387_RS05750 - 1129198..1129818 (-) 621 WP_225666420.1 phage tail protein -
  LA387_RS05755 - 1129849..1130244 (-) 396 WP_225666421.1 DUF806 family protein -
  LA387_RS05760 - 1130241..1130747 (-) 507 WP_225666422.1 HK97 gp10 family phage protein -
  LA387_RS05765 - 1130749..1131099 (-) 351 WP_038603336.1 phage head closure protein -
  LA387_RS05770 - 1131077..1131397 (-) 321 WP_225666424.1 head-tail connector protein -
  LA387_RS05775 - 1131413..1132648 (-) 1236 WP_225666425.1 phage major capsid protein -
  LA387_RS05780 - 1132660..1133364 (-) 705 WP_225667013.1 head maturation protease, ClpP-related -
  LA387_RS05785 - 1133410..1134588 (-) 1179 WP_338085352.1 phage portal protein -
  LA387_RS05790 - 1134585..1134794 (-) 210 WP_015966929.1 DUF1056 family protein -
  LA387_RS05795 - 1134763..1136733 (-) 1971 WP_225666427.1 terminase large subunit -
  LA387_RS05800 - 1136723..1137196 (-) 474 WP_021214441.1 phage terminase small subunit P27 family -
  LA387_RS05805 - 1137324..1137842 (-) 519 WP_225666429.1 HNH endonuclease -
  LA387_RS05815 - 1138792..1139214 (-) 423 WP_225666431.1 RinA family protein -
  LA387_RS05820 - 1139292..1139585 (-) 294 WP_225666433.1 DUF1359 domain-containing protein -
  LA387_RS05825 - 1139636..1139851 (-) 216 WP_216527913.1 hypothetical protein -
  LA387_RS05830 - 1139848..1140036 (-) 189 WP_225666434.1 hypothetical protein -
  LA387_RS05835 - 1140033..1140539 (-) 507 WP_225666435.1 DUF1642 domain-containing protein -
  LA387_RS05840 - 1140837..1141193 (-) 357 WP_225666436.1 DUF1064 domain-containing protein -
  LA387_RS05845 - 1141195..1141644 (-) 450 WP_225666437.1 hypothetical protein -
  LA387_RS05850 dut 1141649..1142068 (-) 420 WP_225666438.1 dUTP diphosphatase -
  LA387_RS05855 - 1142065..1142787 (-) 723 WP_225666439.1 DUF1642 domain-containing protein -
  LA387_RS05860 - 1143042..1143203 (-) 162 WP_225666442.1 hypothetical protein -
  LA387_RS05865 - 1143203..1143541 (-) 339 WP_017370076.1 hypothetical protein -
  LA387_RS05870 - 1143534..1143911 (-) 378 WP_225666444.1 hypothetical protein -
  LA387_RS05875 - 1143920..1144153 (-) 234 WP_225666445.1 DUF1031 family protein -
  LA387_RS05880 - 1144273..1144512 (-) 240 WP_225666446.1 DUF1031 family protein -
  LA387_RS05885 - 1144499..1144903 (-) 405 WP_225666448.1 RusA family crossover junction endodeoxyribonuclease -
  LA387_RS05890 - 1144900..1145049 (-) 150 WP_225666449.1 hypothetical protein -
  LA387_RS05895 - 1145063..1145347 (-) 285 WP_225666450.1 hypothetical protein -
  LA387_RS05900 - 1145331..1146182 (-) 852 WP_225666452.1 phage replisome organizer N-terminal domain-containing protein -
  LA387_RS05905 - 1146182..1146412 (-) 231 WP_040086475.1 helix-turn-helix domain-containing protein -
  LA387_RS05910 ssb 1146517..1146933 (-) 417 WP_225666453.1 single-stranded DNA-binding protein Machinery gene
  LA387_RS05915 - 1146923..1147603 (-) 681 WP_017370188.1 DUF1071 domain-containing protein -
  LA387_RS05920 - 1147615..1148013 (-) 399 WP_225666454.1 hypothetical protein -
  LA387_RS05925 - 1148220..1148474 (-) 255 WP_225666455.1 helix-turn-helix domain-containing protein -
  LA387_RS05930 - 1148567..1148704 (+) 138 WP_165713080.1 hypothetical protein -
  LA387_RS05935 - 1148682..1148828 (-) 147 WP_225666456.1 hypothetical protein -
  LA387_RS05940 - 1148865..1149014 (-) 150 WP_225666457.1 hypothetical protein -
  LA387_RS05945 - 1149039..1149749 (-) 711 WP_225666458.1 ORF6C domain-containing protein -
  LA387_RS05950 - 1149764..1150024 (-) 261 WP_206916930.1 excisionase -
  LA387_RS05955 - 1150038..1150835 (-) 798 WP_206916929.1 phage antirepressor KilAC domain-containing protein -
  LA387_RS05960 - 1150847..1151095 (-) 249 WP_004256843.1 helix-turn-helix domain-containing protein -
  LA387_RS05965 - 1151251..1152075 (+) 825 WP_225666460.1 DUF4393 domain-containing protein -
  LA387_RS05970 - 1152490..1153344 (+) 855 WP_225666462.1 S24 family peptidase -
  LA387_RS05975 - 1153387..1153950 (+) 564 WP_004256839.1 DUF4428 domain-containing protein -
  LA387_RS05980 - 1154072..1155211 (+) 1140 WP_225666464.1 tyrosine-type recombinase/integrase -

Sequence

Protein


Download         Length: 736 a.a.        Molecular weight: 85388.16 Da        Isoelectric Point: 9.7314

>NTDB_id=610846 LA387_RS05680 WP_017370462.1 1109431..1111641(-) (comEC) [Lactococcus garvieae strain Lg-Granada]
MKQKFSLIYFVYLLVFVYFLIFSFSWPLLILSLFSFFIVFYRQYYSVLVLLLCFSLFLLMIKKETEVQERKQPEKISKIT
PYMDTLQVNGSRLSFRGEVNGNDFQAFYTLTSEKEKEYFKKLAVKGTFYFEGTLEIPEEQRNFSGFDYRKYLRTQGVYRL
LKIEKIHGFEEKQDFDLRLVRRKAILWAQEYFPSPMSSYMTGLLFGWLDKDFEEMGELYTSLGIIHLFALSGMQVNFFIE
LLRNILLRLGLSQKLVFFIQIPFSIFYAFLTGLSLSILRALLQKNIPLKNSSDRFSVTFLLLMVFSPYFLLTTGGQLTML
YSFLITFFSRKFKELKGLKKSLLDSTVLAVGVLPLLIFHFHSFQPLSIFLTLLFSLIFDFLLLPGLLFVFLLSLFGTALT
LFNPFFIVLESIVRWVGDLFDQPIVFGKPNLIFLLLLFMLSGLALDFYKKRNWNLSIIIILFLLFFFVKNPKHPSITMVD
VRQGDSILLQDSWNSQNILIDTGGRLKIRADEQWKEGIYKSNAENTLLPYLRARGVSKIDVLIVTHPDEDHIGDLESVAQ
KVPIKNVYVSASALEKKSFAKKLSRLNSQIHIVQEGDKLPIFNSYLWFLTTGVEGKSDNDNSLVTFGEFYGKKFLFTGDL
EEEGEVRLQEEYPHLEVDVLKVGHHGSKTSSKEAFLEAINPKLALISAGKNNRYKHPNQETLQQFAARGIKIYRTDQQGA
IRLLYKKGSWHIQTVK

Nucleotide


Download         Length: 2211 bp        

>NTDB_id=610846 LA387_RS05680 WP_017370462.1 1109431..1111641(-) (comEC) [Lactococcus garvieae strain Lg-Granada]
ATGAAACAAAAATTTTCGCTTATCTATTTCGTTTATCTCTTAGTCTTTGTTTATTTTCTTATTTTTAGTTTTTCATGGCC
TTTACTTATTCTTAGTCTCTTTAGTTTCTTCATCGTTTTTTATCGTCAATACTATAGTGTGTTAGTTCTTTTACTGTGCT
TTAGTTTATTCTTACTCATGATAAAAAAAGAAACGGAAGTACAAGAAAGAAAGCAACCAGAAAAAATTTCGAAAATTACC
CCTTATATGGATACTCTTCAAGTAAATGGAAGCAGGCTCAGTTTCAGAGGAGAAGTGAACGGCAACGACTTCCAAGCTTT
TTACACCTTGACCTCGGAAAAAGAAAAAGAATACTTCAAAAAACTTGCGGTGAAGGGGACTTTTTACTTTGAAGGCACTT
TGGAGATTCCAGAAGAGCAAAGAAACTTTTCAGGTTTTGATTACAGAAAGTATTTAAGGACTCAAGGGGTCTATCGTCTT
CTTAAAATAGAAAAAATTCATGGCTTTGAAGAAAAACAGGACTTTGATTTACGGCTGGTACGCCGAAAAGCCATTCTTTG
GGCTCAGGAATATTTCCCTTCTCCTATGTCGTCCTATATGACAGGGCTTCTTTTTGGGTGGTTGGATAAAGACTTTGAAG
AAATGGGGGAGCTTTACACCAGTCTTGGCATTATCCATTTATTTGCGCTGTCAGGCATGCAGGTGAATTTTTTTATTGAA
CTTCTCCGAAATATTTTACTGCGGCTTGGCTTATCACAGAAGTTAGTCTTTTTTATCCAAATTCCCTTTTCGATATTTTA
TGCATTTCTTACAGGCTTATCTCTTTCAATCTTACGCGCGCTTCTTCAAAAAAATATCCCCCTTAAAAATTCAAGCGATA
GATTTTCTGTCACTTTTCTTCTTTTAATGGTGTTCAGTCCTTATTTTCTGCTGACAACAGGAGGACAACTCACGATGCTT
TATTCTTTCTTAATCACATTTTTTTCACGAAAGTTCAAAGAACTAAAAGGATTAAAAAAATCGTTACTTGATTCTACCGT
CCTAGCTGTTGGGGTTCTTCCGCTTTTGATTTTTCACTTTCATAGTTTTCAGCCATTATCGATTTTCTTGACTTTATTAT
TTAGTCTGATTTTTGATTTTTTACTTCTGCCAGGTTTACTTTTTGTTTTTCTTCTTTCTTTATTCGGTACTGCTCTCACA
TTATTTAATCCTTTTTTTATTGTTTTAGAATCAATAGTAAGATGGGTGGGCGATTTATTTGACCAACCAATTGTCTTTGG
CAAACCCAACCTTATTTTTTTACTTTTACTTTTTATGCTGAGTGGACTTGCCCTTGATTTTTATAAAAAAAGAAACTGGA
ACTTGAGCATAATAATTATTCTTTTCTTGCTTTTTTTCTTTGTTAAGAATCCTAAACACCCAAGCATTACAATGGTAGAT
GTGAGACAAGGGGATAGTATTCTTTTACAAGACAGTTGGAACAGCCAAAACATCCTCATCGATACGGGCGGGAGATTAAA
AATAAGAGCTGATGAGCAGTGGAAGGAGGGAATCTATAAGAGCAATGCCGAAAATACACTTCTGCCTTATCTGAGAGCGC
GTGGCGTGAGTAAGATAGATGTTCTGATTGTGACACATCCAGATGAGGATCATATTGGAGACCTGGAGAGTGTAGCTCAG
AAAGTACCAATAAAAAATGTTTATGTGTCAGCTTCTGCATTGGAGAAGAAAAGCTTTGCTAAAAAACTCAGCCGTTTAAA
CAGTCAGATTCACATTGTGCAGGAGGGGGACAAGCTCCCGATCTTTAACAGTTATCTCTGGTTTTTAACGACAGGCGTTG
AAGGTAAAAGCGACAATGATAATTCTTTAGTGACATTTGGTGAATTTTATGGGAAGAAATTTCTTTTTACAGGTGACTTA
GAAGAAGAGGGAGAGGTGCGGCTCCAGGAAGAATATCCCCATTTAGAGGTGGATGTCCTAAAAGTTGGACATCATGGCAG
TAAAACTTCTTCAAAGGAAGCTTTTTTAGAAGCGATCAACCCTAAGCTCGCTCTTATTTCGGCTGGAAAGAATAATCGAT
ATAAACACCCAAATCAAGAAACGCTCCAACAATTCGCTGCACGAGGCATAAAAATTTATCGTACAGACCAGCAAGGGGCA
ATCCGTCTCCTTTATAAAAAAGGCAGTTGGCACATTCAAACAGTAAAGTAG

Domains

Predicted by InterproScan.

Competence protein (Competence)

(203-445)

Metallo-beta-lactamase superfamily (Lactamase_B)

(481-689)

Domain of unknown function (DUF4131) (DUF4131)

(25-166)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Lactococcus lactis subsp. cremoris KW2

52.66

99.592

0.524
  comEC/celB Streptococcus mitis SK321

44.98

100

0.457
  comEC/celB Streptococcus mitis NCTC 12261

43.548

100

0.44
  comEC/celB Streptococcus pneumoniae TIGR4

43.203

100

0.436
  comEC/celB Streptococcus pneumoniae Rx1

42.799

100

0.432
  comEC/celB Streptococcus pneumoniae D39

42.799

100

0.432
  comEC/celB Streptococcus pneumoniae R6

42.799

100

0.432