NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NMK58_RS27345	Genome accession	NZ_CP101292
Coordinates	5302148..5303272 (-)	Length	374 a.a.
NCBI ID	WP_000228529.1	Uniprot ID	-
Organism	Escherichia coli strain 2003-3014
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 5297148..5308272

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMK58_RS27315 (NMK58_27315)	yrdA	5298948..5299502 (+)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-
NMK58_RS27320 (NMK58_27320)	yrdB	5299478..5299735 (-)	258	WP_001070563.1	DUF1488 domain-containing protein	-
NMK58_RS27325 (NMK58_27325)	aroE	5299732..5300550 (-)	819	WP_000451211.1	shikimate dehydrogenase	-
NMK58_RS27330 (NMK58_27330)	tsaC	5300555..5301127 (-)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NMK58_RS27335 (NMK58_27335)	yrdD	5301132..5301674 (-)	543	WP_001129719.1	type I DNA topoisomerase	-
NMK58_RS27340 (NMK58_27340)	smg	5301703..5302176 (-)	474	WP_000460672.1	DUF494 family protein Smg	-
NMK58_RS27345 (NMK58_27345)	dprA	5302148..5303272 (-)	1125	WP_000228529.1	DNA-protecting protein DprA	Machinery gene
NMK58_RS27350 (NMK58_27350)	def	5303402..5303911 (+)	510	WP_000114986.1	peptide deformylase	-
NMK58_RS27355 (NMK58_27355)	fmt	5303926..5304873 (+)	948	WP_000004477.1	methionyl-tRNA formyltransferase	-
NMK58_RS27360 (NMK58_27360)	rsmB	5304919..5306208 (+)	1290	WP_000744775.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NMK58_RS27365 (NMK58_27365)	trkA	5306230..5307606 (+)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NMK58_RS27370 (NMK58_27370)	mscL	5307736..5308146 (+)	411	WP_000022439.1	large-conductance mechanosensitive channel protein MscL	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40926.86 Da Isoelectric Point: 6.3122

                                >NTDB_id=610143 NMK58_RS27345 WP_000228529.1 5302148..5303272(-) (dprA) [Escherichia coli strain 2003-3014]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=610143 NMK58_RS27345 WP_000228529.1 5302148..5303272(-) (dprA) [Escherichia coli strain 2003-3014]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT

TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.509	98.663	0.479
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	44.865	98.93	0.444
dprA	Vibrio cholerae strain A1552	44.865	98.93	0.444
dprA	Haemophilus influenzae Rd KW20	45.401	90.107	0.409
dprA	Glaesserella parasuis strain SC1401	44.838	90.642	0.406
dprA	Legionella pneumophila strain ERS1305867	44.545	88.235	0.393

Detailed information NTG0610143