Detailed information NTG0610121

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: LCH33_RS13925
• Genome accession: NZ_CP084212
• Coordinates: 3153186..3154355 (+)
• Length: 389 a.a.
• NCBI ID: WP_225231677.1
• Uniprot ID: -
• Organism: Pseudomonas amygdali strain 35-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3148186..3159355

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LCH33_RS13910 (LCH33_002765)	-	3148623..3150950 (+)	2328	WP_057404476.1	ATP-dependent DNA helicase	-
LCH33_RS13915 (LCH33_002766)	pgm	3151066..3152712 (+)	1647	WP_005746410.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
LCH33_RS13920 (LCH33_002767)	-	3152787..3153062 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
LCH33_RS13925 (LCH33_002768)	pilU	3153186..3154355 (+)	1170	WP_225231677.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
LCH33_RS13930 (LCH33_002769)	-	3154404..3155981 (-)	1578	WP_011168500.1	ABC transporter ATP-binding protein	-
LCH33_RS13935 (LCH33_002770)	-	3155983..3157005 (-)	1023	WP_054097554.1	ABC transporter permease	-
LCH33_RS13940 (LCH33_002771)	-	3157005..3158069 (-)	1065	WP_002553338.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42562.74 Da
• Isoelectric Point: 6.0641

>NTDB_id=610121 LCH33_RS13925 WP_225231677.1 3153186..3154355(+) (pilU) [Pseudomonas amygdali strain 35-1]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTPPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=610121 LCH33_RS13925 WP_225231677.1 3153186..3154355(+) (pilU) [Pseudomonas amygdali strain 35-1]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTTGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGACATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTCGATACCCGAAGCTTCCGCGC
GGCGCTGAAGAACACGTTACGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCGATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTCTCGCAGCG
GCTGGTCAGAACCCCGGACGGCAAACGCCGCGCAGCGGTGGAAGTGATGATGGGTACGCCGACCATTCGCGACCTGATCC
AGCGCAACGAACTGACCGAGCTCAAGGGCATCATGGAGAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTCAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGCCGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.172	92.802	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362