Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   NML27_RS02275 Genome accession   NZ_CP101197
Coordinates   484294..485418 (+) Length   374 a.a.
NCBI ID   WP_001551734.1    Uniprot ID   -
Organism   Escherichia coli strain EC21Z-151     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 479294..490418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NML27_RS02250 (NML27_02250) mscL 479420..479830 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  NML27_RS02255 (NML27_02255) trkA 479960..481336 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  NML27_RS02260 (NML27_02260) rsmB 481358..482647 (-) 1290 WP_001551736.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  NML27_RS02265 (NML27_02265) fmt 482693..483640 (-) 948 WP_000004443.1 methionyl-tRNA formyltransferase -
  NML27_RS02270 (NML27_02270) def 483655..484164 (-) 510 WP_000114986.1 peptide deformylase -
  NML27_RS02275 (NML27_02275) dprA 484294..485418 (+) 1125 WP_001551734.1 DNA-protecting protein DprA Machinery gene
  NML27_RS02280 (NML27_02280) smg 485390..485863 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  NML27_RS02285 (NML27_02285) yrdD 485892..486434 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  NML27_RS02290 (NML27_02290) tsaC 486439..487011 (+) 573 WP_001551733.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  NML27_RS02295 (NML27_02295) aroE 487016..487834 (+) 819 WP_001551732.1 shikimate dehydrogenase -
  NML27_RS02300 (NML27_02300) yrdB 487831..488088 (+) 258 WP_001070567.1 DUF1488 domain-containing protein -
  NML27_RS02305 (NML27_02305) yrdA 488064..488618 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -
  NML27_RS02310 (NML27_02310) - 488647..488883 (+) 237 WP_136511987.1 hypothetical protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40934.87 Da        Isoelectric Point: 6.3848

>NTDB_id=609063 NML27_RS02275 WP_001551734.1 484294..485418(+) (dprA) [Escherichia coli strain EC21Z-151]
MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLSWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVAGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=609063 NML27_RS02275 WP_001551734.1 484294..485418(+) (dprA) [Escherichia coli strain EC21Z-151]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACTGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTCTTGGTTGGAGCAACCCAACCATCATTTAATTCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTTGCGACGACAGATTACCCCGGCGCACTGTTTGTTGCAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTAGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCTCCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTAGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCGGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTAACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

48.387

82.888

0.401
  dprA Vibrio cholerae strain A1552

48.387

82.888

0.401
  dprA Haemophilus influenzae Rd KW20

43.917

90.107

0.396
  dprA Legionella pneumophila strain ERS1305867

43.363

90.642

0.393