Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   JF568_RS09395 Genome accession   NZ_CP083931
Coordinates   1899207..1900886 (+) Length   559 a.a.
NCBI ID   WP_097114839.1    Uniprot ID   A0A286EG14
Organism   Alysiella filiformis DSM 16848     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1894207..1905886
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JF568_RS09375 (JF568_09375) - 1895316..1896383 (-) 1068 WP_097114836.1 dsDNA nuclease domain-containing protein -
  JF568_RS09380 (JF568_09380) - 1896529..1897476 (+) 948 WP_097114837.1 nitronate monooxygenase family protein -
  JF568_RS09385 (JF568_09385) zapD 1897569..1898324 (-) 756 WP_097114838.1 cell division protein ZapD -
  JF568_RS09390 (JF568_09390) coaE 1898311..1898952 (-) 642 WP_224446344.1 dephospho-CoA kinase -
  JF568_RS09395 (JF568_09395) pilF 1899207..1900886 (+) 1680 WP_097114839.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JF568_RS09400 (JF568_09400) pilG 1900910..1902193 (+) 1284 WP_097114840.1 type II secretion system F family protein Machinery gene
  JF568_RS09405 (JF568_09405) pilD 1902269..1903111 (+) 843 WP_097114841.1 A24 family peptidase Machinery gene
  JF568_RS09410 (JF568_09410) - 1903320..1904762 (-) 1443 WP_097114842.1 peptide MFS transporter -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61789.74 Da        Isoelectric Point: 5.6761

>NTDB_id=608586 JF568_RS09395 WP_097114839.1 1899207..1900886(+) (pilF) [Alysiella filiformis DSM 16848]
MSIGLLRTLAQQKIVPQNNLEKYQTAIKENKNIIPMLVADKVITPDGLAELLSKLFNYPQLDLNSIPRSKILTDVMDESA
MQENRCVPIFKRGRKVFLAVSDPTQLQNLQKAVFASGLSPDLVIVRDDQLSTVLEWFGQANVSLIKEMGMDASLQEQAAQ
TMIVDGEEEDGPVARFIQKILFDAVNGGASDIHFEFYEFMARVRCRQDGVLREVVQPPVNLRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKGSKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEDFQKKLIMDAIYRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDVNISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDIIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCAACKMPMERPPEKVLLDAGFTKEDLAKDWTM
YRAVGCDACKGKGYKGRAGLYEVMPMTEKMKAVIMKGGTEVDIANIAYEEGLVDLRRSGLLKVMQGITTLDEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=608586 JF568_RS09395 WP_097114839.1 1899207..1900886(+) (pilF) [Alysiella filiformis DSM 16848]
ATGAGCATCGGTTTATTGCGTACCTTGGCACAACAAAAAATTGTGCCACAAAACAACCTAGAAAAATACCAAACAGCCAT
CAAAGAAAACAAAAACATCATTCCCATGCTGGTTGCCGACAAAGTGATTACGCCCGATGGCTTGGCGGAATTGCTGTCCA
AGCTGTTTAACTATCCCCAATTGGATTTAAACAGCATTCCACGCAGCAAAATCCTGACCGATGTGATGGACGAAAGTGCC
ATGCAAGAAAACCGTTGCGTCCCCATTTTCAAGCGCGGACGCAAAGTGTTTTTGGCGGTTTCCGACCCCACCCAGTTGCA
GAATTTGCAAAAAGCCGTGTTTGCAAGTGGCTTGTCGCCCGATTTGGTGATTGTGCGCGATGACCAGTTGAGTACCGTAT
TGGAATGGTTCGGTCAAGCCAACGTGAGCCTGATTAAAGAAATGGGCATGGACGCGAGCTTGCAAGAACAAGCCGCCCAA
ACCATGATTGTGGACGGCGAAGAAGAAGACGGTCCTGTTGCCCGTTTCATTCAAAAGATTTTGTTTGACGCGGTCAATGG
TGGCGCGTCCGATATTCACTTTGAGTTTTACGAATTTATGGCGCGTGTGCGTTGCCGCCAAGACGGTGTGTTGCGTGAAG
TGGTGCAACCGCCCGTAAACTTACGCAGCCAGTTGGCATCGCGCATTAAAGTTATGGCAAAGCTGGACATTTCCGAAAAA
CGTGTCCCACAAGACGGACGCATTCAGTTGCAATTTTCCAAAGGCTCCAAAGCGATTGACTTTCGTGTGAACACCATGCC
CTGCTTGTTTGGCGAAAAGGTGGTAATGCGTATTTTGAACTCAGACGCAGCCAGCCTGAACATCGACCAACTGGGCTTTG
AAGATTTCCAGAAAAAATTGATTATGGACGCGATTTACCGTCCATACGGCATGGTGTTGGTAACAGGGCCTACGGGTTCA
GGTAAAACCGTATCGCTCTACACCTGCCTGAACATTCTGAACACGGACGATGTGAACATTTCCACCGCCGAAGACCCCGC
AGAGATTAACTTACCCGGTATCAACCAAGTCAATGTGAACGACAAACAAGGCTTGACCTTTGAAGCCGCACTCAAAGCCT
TCTTGCGTCAAGACCCTGACATCATCATGATTGGTGAGATTCGCGACTTGGGTACGGCAGACATTGCGATTAAGGCAGCA
CAAACAGGTCATATGGTGTTTTCCACCTTGCACACCAATAACGCCCCTGCTACCTTGTCGCGTATGTTGAACATGGGGGT
TGCGCCCTTTAATATTGCGTCAGCAGTGAATTTGATTATGGCACAACGTTTGGCGCGCCGTTTGTGTGCAGCCTGTAAAA
TGCCGATGGAGCGTCCGCCAGAAAAAGTGCTGTTGGACGCAGGTTTTACCAAAGAAGATTTGGCAAAAGATTGGACAATG
TATCGCGCAGTGGGTTGCGATGCGTGTAAAGGCAAAGGTTACAAAGGTCGTGCAGGTTTGTACGAAGTCATGCCCATGAC
CGAAAAAATGAAAGCCGTGATTATGAAAGGCGGTACCGAAGTGGACATCGCCAACATTGCTTACGAAGAAGGTTTGGTGG
ATTTGCGCCGTTCGGGTTTGCTCAAAGTCATGCAAGGCATTACCACGCTGGACGAAGTCATCGCCACCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286EG14

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

68.036

100

0.682
  pilB Acinetobacter baumannii D1279779

51.858

100

0.524
  pilB Acinetobacter baylyi ADP1

51.246

100

0.515
  pilB Legionella pneumophila strain ERS1305867

47.679

100

0.478
  pilB Vibrio cholerae strain A1552

44.858

100

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

47.059

94.275

0.444
  pilB Vibrio campbellii strain DS40M4

45.92

94.275

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.163

94.097

0.369