NTDB

Overview

Name	dprA	Type	Machinery gene
Locus tag	NMD35_RS02200	Genome accession	NZ_CP100973
Coordinates	455321..456445 (+)	Length	374 a.a.
NCBI ID	WP_000228535.1	Uniprot ID	B7LHY7
Organism	Escherichia coli strain ET300
Function	ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 450321..461445

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMD35_RS02175 (NMD35_02165)	mscL	450447..450857 (-)	411	WP_000022442.1	large-conductance mechanosensitive channel protein MscL	-
NMD35_RS02180 (NMD35_02170)	trkA	450987..452363 (-)	1377	WP_000691382.1	Trk system potassium transporter TrkA	-
NMD35_RS02185 (NMD35_02175)	rsmB	452385..453674 (-)	1290	WP_000744779.1	16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB	-
NMD35_RS02190 (NMD35_02180)	fmt	453720..454667 (-)	948	WP_000004479.1	methionyl-tRNA formyltransferase	-
NMD35_RS02195 (NMD35_02185)	def	454682..455191 (-)	510	WP_000114984.1	peptide deformylase	-
NMD35_RS02200 (NMD35_02190)	dprA	455321..456445 (+)	1125	WP_000228535.1	DNA-protecting protein DprA	Machinery gene
NMD35_RS02205 (NMD35_02195)	smg	456417..456890 (+)	474	WP_000460670.1	DUF494 family protein Smg	-
NMD35_RS02210 (NMD35_02200)	yrdD	456919..457461 (+)	543	WP_001129723.1	type I DNA topoisomerase	-
NMD35_RS02215 (NMD35_02205)	tsaC	457466..458038 (+)	573	WP_001297709.1	L-threonylcarbamoyladenylate synthase type 1 TsaC	-
NMD35_RS02220 (NMD35_02210)	aroE	458043..458861 (+)	819	WP_000451211.1	shikimate dehydrogenase	-
NMD35_RS02225 (NMD35_02215)	yrdB	458858..459115 (+)	258	WP_001070563.1	DUF1488 domain-containing protein	-
NMD35_RS02230 (NMD35_02220)	yrdA	459091..459645 (-)	555	WP_001286216.1	gamma carbonic anhydrase family protein	-

Sequence

Protein

Download Length: 374 a.a. Molecular weight: 40954.92 Da Isoelectric Point: 6.3122

                                >NTDB_id=607997 NMD35_RS02200 WP_000228535.1 455321..456445(+) (dprA) [Escherichia coli strain ET300]

MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE

FYPPQLLVTTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN

GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA

LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDE

VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide

Download Length: 1125 bp

                                >NTDB_id=607997 NMD35_RS02200 WP_000228535.1 455321..456445(+) (dprA) [Escherichia coli strain ET300]

ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG

GCTGGCAAAACAGTCGCAAATTGATGCGGTTGGATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT

CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA

TTTTATCCTCCTCAACTTCTGGTGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC

ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGGTTATTTTGCGAAACTCTGG

CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCTGCCTTACAGGTAAAT

GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGCCATGCCCGACTGGCTGCCAGTCTGCT

TGAACATGGCGGAGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA

TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG

CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA

ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC

CTGAAAATTCATTTTATTCACCAGATCAGCAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG

GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT

AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG

TTTGA

Domains

Predicted by InterProScan.

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)

DprA winged helix domain (WHD_DprA)

(312-357)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	B7LHY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
dprA	Vibrio campbellii strain DS40M4	48.509	98.663	0.479
dprA	Vibrio cholerae O1 biovar El Tor strain E7946	44.865	98.93	0.444
dprA	Vibrio cholerae strain A1552	44.865	98.93	0.444
dprA	Haemophilus influenzae Rd KW20	45.401	90.107	0.409
dprA	Glaesserella parasuis strain SC1401	44.838	90.642	0.406
dprA	Legionella pneumophila strain ERS1305867	44.545	88.235	0.393

Detailed information NTG0607997