Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   NMD37_RS04115 Genome accession   NZ_CP100966
Coordinates   858663..860156 (+) Length   497 a.a.
NCBI ID   WP_000249355.1    Uniprot ID   -
Organism   Escherichia coli strain ET350     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 853663..865156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NMD37_RS04095 (NMD37_04090) pilD 854311..855120 (+) 810 WP_000895883.1 prepilin peptidase PppA Machinery gene
  NMD37_RS04100 (NMD37_04095) gspS2 855186..855596 (+) 411 WP_001345954.1 type II secretion system pilot lipoprotein GspS-beta -
  NMD37_RS04105 (NMD37_04100) gspC 855614..856573 (+) 960 WP_024192083.1 type II secretion system protein GspC -
  NMD37_RS04110 (NMD37_04105) gspD 856603..858663 (+) 2061 WP_223374401.1 type II secretion system secretin GspD -
  NMD37_RS04115 (NMD37_04110) pilB/pilB1 858663..860156 (+) 1494 WP_000249355.1 type II secretion system ATPase GspE Machinery gene
  NMD37_RS04120 (NMD37_04115) gspF 860156..861379 (+) 1224 WP_086259410.1 type II secretion system inner membrane protein GspF -
  NMD37_RS04125 (NMD37_04120) gspG 861396..861851 (+) 456 WP_370598738.1 type II secretion system major pseudopilin GspG -
  NMD37_RS04130 (NMD37_04125) gspH 861855..862418 (+) 564 WP_086259409.1 type II secretion system minor pseudopilin GspH -
  NMD37_RS04135 (NMD37_04130) gspI 862415..862786 (+) 372 WP_086259408.1 type II secretion system minor pseudopilin GspI -
  NMD37_RS04140 (NMD37_04135) gspJ 862783..863388 (+) 606 WP_223374403.1 type II secretion system minor pseudopilin GspJ -
  NMD37_RS04145 (NMD37_04140) gspK 863385..864362 (+) 978 WP_086259406.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55206.37 Da        Isoelectric Point: 6.0390

>NTDB_id=607919 NMD37_RS04115 WP_000249355.1 858663..860156(+) (pilB/pilB1) [Escherichia coli strain ET350]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQAAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=607919 NMD37_RS04115 WP_000249355.1 858663..860156(+) (pilB/pilB1) [Escherichia coli strain ET350]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTGCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGACTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCCCGCCAGCTAATGGAAGATCTCGGTTCTGCTGAGGACTTTTTTACTCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATTCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGCGTTGACGGCACATTACATGAAATGCTGCGC
CCGGGGCGCAAACTGGCCTCGCTGCTGGTATCGCGTATCAAGGTGATGGCACGGCTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCCTCCGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCAGCAACTGACC
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCCGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCACTCGCGCAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATAGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATTGCCGTCCAGGCATCGCTTACAGGGCACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACTGGCGTGATGGCGCAGCGACTGGTTCGTACGCTGTGCCCCGACTGCCGCCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGAATTACCGATGCGCGAACCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGGACTGCCATCCATGAGCTGATCGTGGTGGATGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAGCTGGAGCTGGAACGTTATGTCCGACAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.294

85.513

0.404
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Vibrio cholerae strain A1552

46.465

79.678

0.37
  pilB Acinetobacter baumannii D1279779

46.115

80.282

0.37
  pilF Thermus thermophilus HB27

45

80.483

0.362
  pilB Acinetobacter baylyi ADP1

45.897

78.471

0.36